The mechanism of the gene expression of heat resistant FOK rats in the hot environment

• Project/Area Number: 23592676
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Nagoya City University
• Principal Investigator: MASUDA Kazuhiko 名古屋市立大学, 医学(系)研究科(研究院), 助教 (00381799)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: FOKラット / 高温耐性ラット / 線維芽細胞 / DNA障害 / Pre-heating / 修復能 / DNA二重鎖切断 / ドキソルビシン / 高温耐性 / 体温調節

Outline of Final Research Achievements

Whether fibroblasts of the FOK rats have the character of the heat resistant, exponentially growing cells of the FOK rats and the control WKAH rats were immersed in a water bath maintained at 45℃ for 15, 30, 45, 60, and 75 min. Cell viability of FOK rat fibroblasts showed higher resistant to heat treatment at 45℃ for 15 and 30 min than did control WKAH rat fibroblasts. Furthermore, the both cells were conditioned by preheating (45℃, 8 min), incubating at 37℃ for 10 hours, then exposing to a heat treatment at 45℃ for 15, 30, 45, 60, and 75 min. That preheating increased cell viability of FOK rat fibroblasts at 45℃ for all periods when compared with that of control WKAH rat fibroblasts. It suggests that FOK rat fibroblasts have the character of the heat resistant.