| Project/Area Number |
23592729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
AKIYAMA Masako 東京医科歯科大学, 大学院医歯学総合研究科, 特任助教 (30436646)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAHAMA Ken-ichi 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (60281515)
森田 育男 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60100129)
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| Co-Investigator(Renkei-kenkyūsha) |
MORITA Ikuo 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (60100129)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ドコサヘキサエン酸 / 破骨細胞 / 分化 |
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| Research Abstract |
We performed gene expression analysis using microarrays to identify genes affected by the DHA treatment during osteoclastogenesis. DHA strongly inhibited osteoclastogenesis at the late stage. Among the genes up-regulated by the sRANKL treatment, 4779 genes were down-regulated by DHA and up-regulated by the EPA treatment. Gene ontology analysis identified sets of genes related to cell motility, cell adhesion, cell-cell signaling, and cell morphogenesis. Quantitative PCR analysis confirmed that DC-STAMP, an essential gene for the cell fusion process in osteoclastogenesis, and other osteoclast-related genes such as Siglec-15, Tspan7, and Mst1r were inhibited by DHA.
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