Rotavirus infection to oral epithelial cells

• Project/Area Number: 23592778
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nihon University
• Principal Investigator: ASANO Masatake 日本大学, 歯学部, 准教授 (10231896)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ロタウイルス / 上皮細胞 / インターフェロン

Research Abstract

Rotavirus is a leading cause of the infant virus-related diarreha. The main target of rotavirus infection is intestinal epithelial cells. The purpose of this study is to elucidate whether rotavirus can infect to oral epithelial cells. For this purpose, oral squamous cell carcinoma-derived cell lines were selected. The infectivity of rotavirus was examined by both real-time PCR and immunofluoresence cell staining. The results clearly indicated that rotavirus can infect oral epithelial cells. Using intestinal adenocarcinoma cell line, HT-29, the production of type III interferon was also examined. The results indicated that rotavirus infection leads to type III interferon production mainly through NF-kB pathway. Rotavirus infection also leads to the production of type I interferon. The comparison between type I and type III interferons revealed that type III interferon was produced much higher amount but the anti-viral effect was much profound in type I interferon.

Research Products

• [Journal Article] Nickel ion inhibits nuclear factor-kappa B activity in human oral squamous cell carcinoma (2013)
  • Author(s): Shionome T, Endo S, Omagari D, Asano M, Toyoma H, Ishigami T, Komiyama K.
  • Journal Title: PLOS one Volume: 8
  • Peer Reviewed
• [Journal Article] Distinct functional regions of human polymeric immunoglobulin receptor (2013)
  • Author(s): Sunagawa K, Omagari D, Nishiyama M, Asano M, Okudera M, Sugitani M, Nemoto N, Komiyama K.
  • Journal Title: Scand J Immunol Volume: 78 Pages: 339-344
  • Peer Reviewed
• [Journal Article] Nicotine-induced expression of low density lipoprotein receptor in oral epithelial cells. (2013)
  • Author(s): Ito S, Gojoubori T, Yamaguchi Y, Asano M, , Goke E, Koshi R, Sugano N, Yoshinuma N, Komiyama K, Ito K.
  • Journal Title: PLOS one Volume: 8
  • Peer Reviewed
• [Journal Article] Distinct signaling pathways leading to the induction of human β-defensin 2 by stimulating an electrolyticaly-generated acid functional water and double strand RNA in oral epithelial cells. (2013)
  • Author(s): Gojoubori T, Nishio Y, Asano M, Nishida T, Komiyama K, Ito K.
  • Journal Title: J Recept Signal Transduct Res Volume: 34 Pages: 97-103
  • Peer Reviewed
• [Journal Article] Nuclear factor kappa B plays a pivotal role in polyinosinic-polycytidylic acid-induced expression of humanβ-defensin 2 in intestinal epithelial cell (2011)
  • Author(s): Omagari D, Takenouchi-Ohkubo N, Endo S, Ishigami T, Sawada A, Moro I, Asano M, Komiyama K
  • Journal Title: Clin Exp Immunol Volume: 165 Pages: 85-93
  • Peer Reviewed
• [Presentation] EVALUATION OF PREVENTIVE EFFECT OF TYPE I AND TYPE III IFN ON ROTAVIRUS INFECTION (2013)
  • Author(s): Sato G, Gojoubori T, Omagari D, Asano M, Komiyama K
  • Organizer: 5^<th> European rotavirus biology meeting
  • Place of Presentation: Valencia (Spain)
  • Year and Date: 2013-10-07
• [Presentation] ASSESSMENT OF ANTI-VIRAL ACTIVITY OF ACID-ELECTROLYZED FUNCTIONAL WATER (FW) (2013)
  • Author(s): Asano M, Iwasa S, Nishiyama M, Sato G, Gojoubori T, Komiyama K
  • Organizer: 5^<th> European rotavirus biology meeting
  • Place of Presentation: Valencia (Spain)
  • Year and Date: 2013-10-06
• [Presentation] Assessment of anti-viral activity of acid electrolyzed functional water. (2013)
  • Author(s): Asano M, Iwasa S, Nishiyama M, Sato G, Gojoubori T, Komiyama K.
  • Organizer: 5th European Rotavirus Meeting
  • Place of Presentation: Valensia, Spain
• [Presentation] PRODUCTION OF TYPE III IFNS BY ROTAVIRUS INFECTED INTESTINAL EPITHELIAL CELLS (2012)
  • Author(s): Asano M, Omagari D, Gojoubori T, Komiyama K
  • Organizer: 11^<th> International symposium on double-stranded RNA viruses
  • Place of Presentation: Puerutoriko (USA)
  • Year and Date: 2012-11-30
• [Presentation] Production of type III IFNs in rotavirus infected intestinal epithelial cells (2012)
  • Author(s): Asano M
  • Organizer: The 11th International Symposium on Double-stranded RNA virus
  • Place of Presentation: Pueruto Rico, USA
• [Presentation] Rotavirus infection and type III interferon (2011)
  • Author(s): Asano M
  • Organizer: 15th International Congress of Mucosal Immunology
  • Place of Presentation: Paris, France