Establishment of saliva metabolome analysis using mice experimental cancer model

• Project/Area Number: 23592784
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tsurumi University
• Principal Investigator: SHIBATA Tatsuya 鶴見大学, 歯学部, 助教 (90323708)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Akemi 鶴見大学, 歯学部, 助教 (00339813) ; NAKASHIMA Kazuhisa 鶴見大学, 歯学部, 非常勤講師 (90252692) ; SUGIMOTO Masahiro 慶應義塾大学, 政策メディア研究科, 准教 (30458963) ; NIFUJI Akira 鶴見大学, 歯学部, 教授 (00240747)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: メタボローム / 唾液 / 腫瘍 / 癌 / 唾液メタボローム

Research Abstract

Saliva contains components originating from blood but it is only applicated to diagnosis in specific diseases. Metabolome analysis aims to comprehensively identify and quantify metabolites in bodily fluids. To diagnose diseases with the salivary metabolome analysis, it should be clear whether or not there is the metabolome profile difference between saliva and blood, and whether or not a disease affects the metabolome profile.
In this study, we compared the metabolome profiles of saliva and blood in mice and many metabolites were consistently detected in both saliva and blood, indicating that metabolites in blood transfer to saliva. Based on this result, we are analyzing the salivary metabolome profile in experimental mice cancer model.

Research Products

• [Journal Article] Alendronate promotes bone formation by inhibiting protein prenylation in osteoblasts in rat tooth replantation model. (2013)
  • Author(s): Komatsu K, Shimada A, Shibata T, Wada S, Ideno H, Nakashima K, Amizuka N, Noda M, Nifuji A.
  • Journal Title: J Endocrinology Volume: 219 Pages: 145-158
  • DOI: 10.1530/joe-13-0040
  • Peer Reviewed
• [Journal Article] Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells. (2013)
  • Author(s): Ryoko Araki
  • Journal Title: Nature. Volume: 494 Pages: 100-104
  • DOI: 10.1038/nature11807
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of skeletal tissue formation (2013)
  • Author(s): Nifuji A
  • Journal Title: The Journal of Physical Fitness and Sports Medicine Volume: 2 Pages: 1-8
  • URL: http://www.jspfsm.umin.ne.jp/journal/
  • Peer Reviewed
• [Journal Article] Predominant expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development. (2013)
  • Author(s): Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, and Nifuji A
  • Journal Title: Gene Expr Patterns. Volume: 13 Pages: 84-90
  • DOI: 10.1016/j.gep.2013.01.002
  • Peer Reviewed
• [Journal Article] Metabolome analysis of erythrocytes from patients with chronic hepatitis C reveals the etiology of ribavirin-induced hemolysis (2013)
  • Author(s): Karasawa, T., Saito, T., Ueno, Y., Sugimoto, M., Soga, T.
  • Journal Title: International Journal of Medical Sciences Volume: 10 Pages: 1575-1577
  • DOI: 10.7150/ijms.6436
  • Peer Reviewed
• [Journal Article] Dynamics of serum metabolites in patients with chronic hepatitis C receiving pegylated interferon plus ribavirin: a metabolomics analysis (2013)
  • Author(s): Saito, T., Sugimoto, M., Igarashi, K., Saito, K., Shao, L., Katsumi, T., Tomita, K., Sato, C., Ishii, R., Okumoto, K., Nishise, Y., Watanabe, H., Tomita, M., Ueno, Y., Soga, T.
  • Journal Title: Metabolism Volume: 62 Pages: 1577-1586
  • DOI: 10.1016/j.metabol.2013.07.002
  • Peer Reviewed
• [Journal Article] High-Resolution-Imaging Mass Spectrometry Reveals Detailed Spatial Distribution of Phosphatidylinositols in Human Breast Cancer (2013)
  • Author(s): Kawashima M., Iwamoto N., Kawaguchi-Sakita, N., Sugimoto M., Ueno T., Mikami Y. Terasawa K., Sato, T., Tanaka K., Shimizu K., Toi M.
  • Journal Title: Cancer Science Volume: 104 Pages: 1372-9
  • Peer Reviewed
• [Journal Article] Changes of Metabolic Profiles in an Oral Squamous Cell Carcinoma Cell Line Induced by Eugenol (2013)
  • Author(s): Koh T., Murakami Y., Machino M., Onuma H., Kaneko M., Sugimoto M., Soga T., Tomita M.
  • Journal Title: In Vivo Volume: 27 Pages: 233-243
  • URL: http://iv.iiarjournals.org/content/27/2/233.full.pdf+html
  • Peer Reviewed
• [Journal Article] High-resolution imaging mass spectrometry reveals detailed spatial distribution of phosphatidylinositols in human breast cancer (2013)
  • Author(s): Kawashima M., Iwamoto N., Kawaguchi-Sakita, N., Sugimoto M., Ueno T., Mikami Y. Terasawa K., Sato, T., Tanaka K., Shimizu K., Toi M.
  • Journal Title: Cancer Science Volume: 104 Pages: 1372-1379
  • DOI: 10.1111/cas.12229
  • Peer Reviewed
• [Journal Article] Improved methods for detection ofβ-galactosidase (lacZ) activity in hard tissue (2012)
  • Author(s): Shimada A, Komatsu K, Nakashima K, Poschl E, Nifuji A
  • Journal Title: Histochem Cell Biol Volume: 137 Pages: 841-7
  • DOI: 10.1007/s00418-012-0936-1
  • Peer Reviewed
• [Journal Article] Pilot study of changes in salivary metabolic profiles induced by template therapy (2012)
  • Author(s): Tanaka S., Taga H., Maehara K., Kaneshima A., Machino M., Onuma H., Kaneko M., Sakagami H.
  • Journal Title: In Vivo Volume: 26(6) Pages: 1015-20
  • URL: http://iv.iiarjournals.org/content/26/6/1015.long
  • Peer Reviewed
• [Journal Article] Metabolic profiling reveals new serum biomarkers for differentiating diabetic nephropathy (2012)
  • Author(s): Hirayama A., Nakashima E., Sugimoto M., Akiyama S., Sato W., Maruyama S., Matsuo S., Tomita M., Yuzawa Y., Soga T.
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 404(10) Pages: 3101-9
  • Peer Reviewed
• [Journal Article] Human blood metabolite timetable indicates internal body time. (2012)
  • Author(s): Kasukawa T, Sugimoto M, Hida A, Minami Y, Mori M, Honma S, Honma K, Mishima K, Soga T, Ueda HR
  • Journal Title: Proceedings of the National Academy of Sciences USA Volume: 109(37) Pages: 15036-15041
  • DOI: 10.1073/pnas.1207768109
  • Peer Reviewed
• [Journal Article] Bioinformatics Tools for Mass Spectroscopy-Based Metabolomic Data Processing and Analysis (2012)
  • Author(s): Sugimoto M., Kawakami M., Robert M., Soga T., Tomita M.
  • Journal Title: Current Bioinformatics Volume: 7(1) Pages: 96-106
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299976/
  • Peer Reviewed
• [Journal Article] Predictions of the pathological response to neoadjuvant chemotherapy in patients with primary breast cancer using a data mining technique (2012)
  • Author(s): Takada M., Sugimoto M., Ohno S., Kuroi K., Sato N., Bando H., Masuda N., Iwata H., Kondo M., Sasano H., Chow L.W.C., Inamoto T., Naito Y., Tomita M., and Toi M.
  • Journal Title: Breast Cancer Research and Treatment Volume: 134 Pages: 661-70
  • DOI: 10.1007/s10549-012-2109-2
  • Peer Reviewed
• [Journal Article] CE-MS–based metabolome analysis of serum and saliva from neurodegenerative dementia patients
  • Author(s): Tsuruoka M., Hara J., Hirayama A., Sugimoto M., Soga T., Shankle W.R., Tomita M.
  • Journal Title: Electrophoresis
  • DOI: 10.1002/elps.201300019
  • Peer Reviewed
• [Presentation] 代謝産物の血液から唾液への移行についての検討 (2013)
  • Author(s): 柴田達也、杉本昌弘、金子未来、島田明美、小松浩一郎、出野尚、中島和久、冨田勝、二藤彰
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Endocytic incorporation of alendronate promotes bone formation (2013)
  • Author(s): Komatsu K, Shimada A, Ideno H, Shibata T, Nakashima K, Amizuka N, Noda M, Nifuji A
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe Japan
• [Presentation] 代謝産物の血液から唾液への移行についての検討 (2013)
  • Author(s): 柴田達也、杉本昌弘、金子未来、島田明美、小松浩一郎、出野 尚、中島和久、冨田 勝、二藤 彰
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] Endocytic incorporation of alendronate promotes bone formation. (2013)
  • Author(s): Komatsu K, Shimada A, Ideno H, Shibata T, Nakashima K, Amizuka N, Noda M, Nifuji A.
  • Organizer: 国際骨代謝学会（IBMS）・日本骨代謝学会（JSBMR）第2回合同国際会議
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] アレンドロネートの細胞内取込みによる骨形成の促進 (2012)
  • Author(s): 小松浩一郎、島田明美、出野尚、柴田達也、中島和久、二藤彰
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京