Acquirement of amelogenic stem/progenitor cells for bio-engineered tooth

• Project/Area Number: 23592875
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KUREMOTO Koh-ichi 広島大学, 医歯薬保健学研究院, 助教 (90319583)
• Co-Investigator(Renkei-kenkyūsha): YAMAZA Takayoshi 九州大学, 歯学研究院, 講師 (80304814)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 口腔上皮 / 幹細胞 / 再生 / エナメル質

Research Abstract

The purpose of this study is to acquire the amelogenic stem/progenitor cells for the bio-engineered tooth regeneration. This study might be the basis for the study of enamel regeneration using the dental epithelial stem cells (DESCs) and contribute greatly to the development and clinical application of the bio engineered tooth.
We used rodent incisors which regenerate throughout the animal lifetime as a result of the continuous deposition of enamel by ameloblasts. Putative dental epithelial stem cells (DESCs) were isolated from the base of the incisors in a structure called the cervical loop (CL). We demonstrated that the enzyme dissociation method was quite helpful in the isolation of DESCs from the CL and collagen I coating on the surface of the culture plate and MCDB153 medium with mEGF were quite useful for DESCs culture.
In the future, it will be important to further characterize DESCs for the optimum culture condition.

Research Products

• [Journal Article] Immune Therapeutic Potential of Stem Cells from Human Supernumerary Teeth (2013)
  • Author(s): Makino Y, 他9名. Yamaza T
  • Journal Title: J Dent Res Volume: 92(7) Issue: 7 Pages: 609-15
  • DOI: 10.1177/0022034513490732
  • Peer Reviewed
• [Journal Article] Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine (2012)
  • Author(s): Ma L, 他9名 Yamaza T
  • Journal Title: PLoS One Volume: 7, e51777 Issue: 12 Pages: 12-12
  • DOI: 10.1371/journal.pone.0051777
  • Peer Reviewed
• [Journal Article] Core binding factor beta functions in the maintenance of stem cells and orchestrates continuous proliferation and differentiation in mouse incisors (2011)
  • Author(s): Kurosaka H, Islam MN, Kuremoto K, Hayano S, Nakamura M, Kawanabe N, Yanagita T, Rice DP, Harada H, Taniuchi I, Yamashiro T
  • Journal Title: Stem Cells Volume: 29巻 Issue: 11 Pages: 1792-1803
  • DOI: 10.1002/stem.722
  • Peer Reviewed
• [Presentation] Nano-architecture Formation on the Surface of Ti based Metals by Solution Chemical Processing (2012)
  • Author(s): Kaga K, Sekino T, Nishida H, Honda Y, Kuremoto K, 他
  • Organizer: The 5th International Symposium Functional Materials
  • Place of Presentation: Perth, Australia
  • Year and Date: 2012-12-20
• [Presentation] インプラントを併用した新しい部分床義歯治療のための支台装置の開発 (2012)
  • Author(s): 呉本晃一, 他
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-10
• [Presentation] 溶液化学反応場によるTi合金表面の低次元酸化物ナノ構造の形成 (2012)
  • Author(s): 加賀晃樹, 関野徹, 田中俊一郎, 西田尚敬, 呉本晃一
  • Organizer: 日本セラミックス協会第25回秋季シンポジウム
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-09-20
• [Presentation] Low-dimensional Oxide Nanostructures Formation on Metal Surface via Low-temperature Eco-processing (2012)
  • Author(s): Sekino T, Kaga K, Nishida H, Kuremoto K, 他
  • Organizer: International Union of Materials Research Society–International Conference in Asia
  • Place of Presentation: Busan, South Korea
  • Year and Date: 2012-08-29
• [Presentation] 常温での溶液化学反応場による金属表面の低次元ナノ構造構築 (2012)
  • Author(s): 加賀晃樹, 関野徹, 西田尚敬, 呉本晃一, 他
  • Organizer: 社団法人日本金属学会2012年春期(第150回)大会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-03-29
• [Presentation] 常温での溶液化学反応場によるTi合金表面の低次元ナノ構造構築 (2012)
  • Author(s): 加賀晃樹, 関野徹, 西田尚敬, 呉本晃一, 他
  • Organizer: 公益社団法人日本セラミックス協会2012年年会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-21
• [Presentation] 線維芽細胞増殖因子が口腔上皮由来幹細胞の機能と分化を制御する (2011)
  • Author(s): 呉本晃一, 他
  • Organizer: 日本補綴歯科学会第120回記念学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2011-05-22
• [Presentation] 線維芽細胞増殖因子が口腔上皮由来幹細胞の機能と分化を制御する (2011)
  • Author(s): 呉本晃一、前田照太、西崎 宏、岡崎定司
  • Organizer: 日本補綴歯科学会 第120回記念学術大会
  • Place of Presentation: 広島国際会議場
• [Book] インプラントを併用した新しい部分床義歯治療のための支台装置の開発, 歯界展望, 2013特別号 (2013)
  • Author(s): 呉本晃一, 他
  • Total Pages: 282
  • Publisher: 医歯薬出版株式会社(東京)