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Evaluation of a dental pulp-derived cell sheet cultured on an amniotic membrane substrate

Research Project

Project/Area Number 23592934
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

YAMAMOTO Toshiro  京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472)

Co-Investigator(Kenkyū-buntansha) KANAMURA Narisato  京都府立医科大学, 医学研究科, 准教授 (70204542)
KITA Masakazu  京都府立医科大学, 医学研究科, 准教授 (60153087)
AMEMIYA Takeshi  京都府立医科大学, 医学研究科, 助教 (90398389)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords羊膜上培養歯髄細胞シート / 羊膜 / 歯髄由来細胞 / 免疫組織化学 / 移植・再生医療 / 再生医学 / 歯学 / 細胞・組織 / 羊膜上培養歯髄由来細胞シート
Research Abstract

Dental pulp cells collected from extracted wisdom teeth were cultured on an amniotic membrane, which is usually discarded after delivery. As a result, a cell sheet was successfully created. The cell sheet cultured on the amniotic membrane contained mesenchymal stem cells and had a bone differentiation potential. Thus, the dental pulp cell sheet had a tissue regeneration potential from the dental pulp stem cells on the cell sheet.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Presentation (13 results) Remarks (2 results)

  • [Presentation] Histological examination of pulp cell sheet cultured using amniotic membrane2013

    • Author(s)
      Yamamoto T, Kanamura N
    • Organizer
      35th Asia Pacific Dental Congress
    • Place of Presentation
      Malaysia
    • Related Report
      2013 Final Research Report
  • [Presentation] 羊膜上培養歯髄由来細胞シートの骨分化に関する検討2013

    • Author(s)
      山本俊郎、本城賢一、市岡宏顕、足立圭司、雨宮傑、中村亨、金村成智
    • Organizer
      第56回春季日本歯周病学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 羊膜上培養歯髄由来細胞シートの作成及び骨分化能に関する検討2013

    • Author(s)
      山本俊郎、本城賢一、熊本園子、雨宮傑、金村成智
    • Organizer
      第34回日本炎症・再生医学会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 羊膜を基質とした培養歯髄由来細胞シートの作成及び骨分化能に関する検討2013

    • Author(s)
      本城賢一、山本俊郎、西野悠貴、木村功、雨宮傑、中井道明、金村成智
    • Organizer
      第58回日本口腔外科学会総会・学術大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Histological examination of pulp cell sheet cultured using amniotic membrane2013

    • Author(s)
      Toshiro Yamamoto, Narisato Kanamura
    • Organizer
      35TH ASIA PACIFIC DENTAL CONGRESS
    • Place of Presentation
      マレーシア
    • Related Report
      2013 Annual Research Report
  • [Presentation] 羊膜上培養歯髄由来細胞シートの作成2013

    • Author(s)
      山本俊郎、本城賢一、市岡宏顕、足立圭司、雨宮 傑、金村成智
    • Organizer
      第55回春季日本歯周病学会学術大会
    • Place of Presentation
      北海道
    • Related Report
      2012 Research-status Report
  • [Presentation] 歯周組織の再生を目指した羊膜上培養歯髄由来細胞シート作成 (第12回日本抗加齢医学会総会2012優秀演題受賞)2013

    • Author(s)
      本城賢一、山本俊郎、市岡宏顕、足立圭司、雨宮 傑、喜多正和、金村成智
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 羊膜上培養歯髄由来細胞シートの免疫組織化学的検討2013

    • Author(s)
      山本俊郎、本城賢一、市岡宏顕、山本健太、赤松佑紀、足立圭司、大迫文重、雨宮 傑、喜多正和、金村成智
    • Organizer
      第136回日本歯科保存学会2012年度春季大会
    • Place of Presentation
      沖縄
    • Related Report
      2012 Research-status Report
  • [Presentation] 羊膜上培養歯髄由来細胞シートの作成2012

    • Author(s)
      山本俊郎、本城賢一、市岡宏顕、足立圭司、雨宮傑、金村成智
    • Organizer
      第55回春季日本歯周病学会学術大会
    • Place of Presentation
      北海道
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯周組織の再生を目指した羊膜上培養歯髄由来細胞シート作成2012

    • Author(s)
      本城賢一、山本俊郎、市岡宏顕、足立圭司、雨宮傑、喜多正和、金村成智
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Cultured of pulp-derived cells on human amniotic membrane2012

    • Author(s)
      Yamamoto T, Honjo K, Ichioka H, Amemiya T, Mazda O, Kita M, Kanamura N
    • Organizer
      The 90th General Session and Exhibition of the International Association for Dental Research
    • Place of Presentation
      Brazil
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 羊膜上培養歯髄由来細胞シートの免疫組織化学的検討2012

    • Author(s)
      山本俊郎、本城賢一、市岡宏顕、山本健太、赤松佑紀、足立圭司、大迫文重、雨宮傑、喜多正和、金村成智
    • Organizer
      第136回日本歯科保存学会2012年度春季大会
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] 羊膜を基質とした培養歯髄由来細胞シートの作成2011

    • Author(s)
      山本俊郎、本城賢一、市岡宏顕、山本健太、赤松佑紀、足立圭司、雨宮傑、喜多正和、金村成智
    • Organizer
      第135回日本歯科保存学会2011年度秋季大会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Remarks] 第12回日本抗加齢医学会総会2012優秀 演題受賞

    • Related Report
      2013 Final Research Report
  • [Remarks] 35^<th> APDC Poster Presentation 1^<st> Prize受賞

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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