Basic research about bone regeneration and oral implant for craniofacial defect patients

• Project/Area Number: 23593054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: NAKAMURA Hiroshi 松本歯科大学, 歯学部, 講師 (00278178)
• Co-Investigator(Kenkyū-buntansha): MIYAZAWA Hiroo 松本歯科大学, 総合歯科医学研究所, 教授 (90147637) ; NAKAMURA Midori 松本歯科大学, 歯学部, 准教授 (90278177) ; YAGAMI Kimitoshi 松本歯科大学, 歯学部附属病院, 准教授 (00210211) ; UDAGAWA Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801) ; NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨芽細胞 / 破骨細胞 / 遺伝子欠損マウス / 口蓋裂 / 骨再生 / Rykシグナル / Wntシグナル / 骨誘導因子 / 顎裂 / Ryk欠損マウス / 骨形成 / 骨吸収 / 再生医療

Research Abstract

We generated systemic Ryk signal deficient mice. Ryk deficient mice exhibited a cleft palate and totally decrease of osteoclast number and osteoblast number in bone. Furthermore, we generated osteoclast-specific Ryk-deficient mice and osteoblast-specific Ryk-deficient mice. Interestingly, there are no cleft palate in both osteoclast-specific Ryk-deficient mice and osteoblast-specific Ryk-deficient mice. Osteoclast-specific Ryk-deficient mice have normal bone phenotype. These results suggest that Ryk expression in osteoclasts and osteoblasts is not essential for craniofacial defect.

Research Products

• [Journal Article] Arctigenin inhibits osteoclast differentiation and function by suppressing both calcineurin-dependent and osteoblastic cell-dependent NFATc1 pathways. (2014)
  • Author(s): Yamashita T, Uehara S, Udagawa N, Li F, Kadota S, Esumi H, Kobayashi Y, Takahashi N
  • Journal Title: PLoS One Volume: 9 Issue: 1 Pages: 85878-85878
  • DOI: 10.1371/journal.pone.0085878
  • Peer Reviewed / Open Access
• [Journal Article] VitaminD endocrine system and osteoclasts (2014)
  • Author(s): Takahashi N, Udagawa N, Suda T
  • Journal Title: BoneKEy Reports Volume: 3 Pages: 495-495
  • DOI: 10.1038/bonekey.2013.229
  • Peer Reviewed / Open Access
• [Journal Article] Osteoclast Cell Lineage : Characteristics and behavior of osteoclast precursors in vivo (2014)
  • Author(s): Takahashi N, Kobayashi Y, Nakamura M, Udagawa N (他3名)
  • Journal Title: Encyclopedia of Human Body 3rd Edition Volume: (in press)
  • Peer Reviewed
• [Journal Article] Noncanonical Wnt5a enhances Wnt/-catenin signaling during osteogenesis. (2014)
  • Author(s): Okamoto, M., Udagawa, N., Yamashita, T., Nakamichi, Y., Uehara, S., Kato, H., Saito, N., Minami, Y., Takahashi, N., Kobayashi, Y.
  • Journal Title: Sci. report Volume: 4 Issue: 1 Pages: 4493-4493
  • DOI: 10.1038/srep04493
  • Peer Reviewed / Open Access
• [Journal Article] VitaminD endocrine system and osteoclasts. (2014)
  • Author(s): Takahashi N, Udagawa N, Suda T
  • Journal Title: BoneKEy Reports Volume: 3 Pages: 495-495
  • Peer Reviewed
• [Journal Article] Roles of cathelicidin-related antimicrobial peptide in murine osteoclastogenesis (2013)
  • Author(s): Horibe K, Kobayashi Y, Takahashi N, Udagawa N(他4名)
  • Journal Title: Immunology Volume: 140 Issue: 3 Pages: 344-351
  • DOI: 10.1111/imm.12146
  • Peer Reviewed / Open Access
• [Journal Article] Osteoprotegerin-deficient male mice as a model for severe alveolar bone loss: Comparison with RANKL-overexpressing transgenic male mice. (2013)
  • Author(s): Koide M et al.
  • Journal Title: Endocrinology Volume: 190 Issue: 2 Pages: 773-782
  • DOI: 10.1210/en.2012-1928
  • Peer Reviewed / Open Access
• [Journal Article] Stimulation of bone formation in cortical bone of mice treated with a receptor activator of nuclear factor-κB ligand (RANKL)-binding peptide that possesses osteoclastogenesis inhibitory activity. (2013)
  • Author(s): Furuya Y, Inagaki A, Khan M, Mori K, Penninger JM, Nakamura M, Udagawa N, Aoki K, Ohya K, Uchida K, Yasuda H
  • Journal Title: J Biol Chem Volume: 288 Issue: 8 Pages: 5562-5571
  • DOI: 10.1074/jbc.m112.426080
  • Peer Reviewed
• [Journal Article] IL-34 and CSF-1 similarities and differences (2013)
  • Author(s): Nakamichi Y, Udagawa N, Takahashi N
  • Journal Title: J Bone Miner Metab Volume: 31 Issue: 5 Pages: 486-495
  • DOI: 10.1007/s00774-013-0476-3
  • NAID: 10031201682
  • Peer Reviewed / Open Access
• [Journal Article] Neural-inducing factor Netrin-1 is regulated in chondrogenesis and osteogenesis by BMP or Noggin (2012)
  • Author(s): Yagami K, Nakamura M, Nakamura H, Udagawa N(他3名)
  • Journal Title: J Hard Tissue Biology Volume: 21 Pages: 141-150
  • NAID: 120006576210
  • URL: http://mol.medicalonline.jp/archive/search?jo=dq4hdtib&ye=2012&vo=21&issue=2
  • Peer Reviewed
• [Journal Article] Wnt5a-Ror2 signaling between osteoblasts and osteoclast precursors enhances osteoclastogenesis (2012)
  • Author(s): Maeda, K., Kobayashi, Y., Udagawa, N., Uehara, S., Ishihara, A., Mizoguchi, T., Kikuchi, Y., Takada, I., Kato, S., Kani, S., Nishita, M., Marumo, K., Martin, T.J., Minami, Y., Takahashi, N.
  • Journal Title: Nat.Med. Volume: (印刷中) Issue: 3 Pages: 405-12
  • DOI: 10.1038/nm.2653
  • Peer Reviewed
• [Journal Article] New roles of osteoblasts involved in osteoclast differentiation. (2012)
  • Author(s): Yamashita T, Takahashi N, Udagawa N
  • Journal Title: World J Osteoarthritis Volume: 3 Issue: 11 Pages: 175-181
  • DOI: 10.5312/wjo.v3.i11.175
  • Peer Reviewed
• [Journal Article] Neural-inducing factor Netrin-1 is regulated in chondrogenesis and osteogenesis by BMP or Noggin. (2012)
  • Author(s): Yagami K, Sadaoka S, Nakamura M, Nakamura H, Maki S, Yagasaki T and Udagawa N
  • Journal Title: J Hard Tissue Biol Volume: 21 Pages: 141-150
  • NAID: 120006576210
  • Peer Reviewed
• [Journal Article] Lineage-Committed Osteoclast Precursors Circulate in Blood and Settle Down Into Bone (2011)
  • Author(s): Muto, A, Mizoguchi, T, Udagawa, N., Ito, S, Kawahara, I, Abiko, Y, Arai, A., Harada, S., Kobayashi, Y., Nakamichi, Y, Penninger, JM, Noguchi, T, Takahashi, N.
  • Journal Title: Journal of Bone and Mineral Research Volume: 26 Issue: 12 Pages: 2978-2990
  • DOI: 10.1002/jbmr.490
  • Peer Reviewed
• [Journal Article] Rab27a and Rab27b are involved in stimulation-dependent RANKL release from secretory lysosomes in osteoblastic (2011)
  • Author(s): Kariya Y, et al
  • Journal Title: J Bone Miner Res Volume: 26 Issue: 4 Pages: 689-703
  • DOI: 10.1002/jbmr.268
  • Peer Reviewed
• [Journal Article] Hyergravity suppresses bone resorption in ovariectomized rat (2011)
  • Author(s): Ikawa T, et al
  • Journal Title: Adv Space Res Volume: 47 Issue: 7 Pages: 1214-1224
  • DOI: 10.1016/j.asr.2010.12.004
  • Peer Reviewed
• [Presentation] 神経成長因子Netrin-1のBMPとNogginによる軟骨細胞および骨芽細胞分化における役割 (2014)
  • Author(s): 中村浩志
  • Organizer: 第52回日本小児歯科学会
  • Place of Presentation: 東京都
  • Year and Date: 2014-05-17
• [Presentation] 低分子ポリフェノールの口腔カンジダ症に対する作用の検討 (2013)
  • Author(s): 中村浩志
  • Organizer: 第51回日本小児歯科学会
  • Place of Presentation: 岐阜市
  • Year and Date: 2013-05-24
• [Presentation] BMP誘導性異所性骨形成における破骨細胞の役割 (2013)
  • Author(s): 中村美どり
  • Organizer: 第51回日本小児歯科学会
  • Place of Presentation: 岐阜市
  • Year and Date: 2013-05-24
• [Presentation] Oligonol(R)のStreptococcus mutansおよびPorphyromonas gingivalisに対する薬理作用の検討 (2012)
  • Author(s): 中村浩志
  • Organizer: 第50回日本小児歯科学会
  • Place of Presentation: 東京都
  • Year and Date: 2012-05-13
• [Presentation] Osteoclastic bone resorption directly activates osteoblast function. (2011)
  • Author(s): Udagawa N
  • Organizer: 1nd Bio-Rheumatology International Congress Tokyo 8th GARN Meeting Tokyo(招待講演)
  • Place of Presentation: Urayasu, Chiba, Japan
• [Presentation] 神経成長因子Netrin-1のBMPとNogginによる軟骨細胞および骨芽細胞分化における役割
  • Author(s): 中村浩志
  • Organizer: 第52回日本小児歯科学会
  • Place of Presentation: 東京(品川区立総合区民会館)
• [Presentation] 低分子ポリフェノールの口腔カンジダ症に対する作用の検討
  • Author(s): 中村浩志
  • Organizer: 第51回日本小児歯科学会
  • Place of Presentation: 岐阜(長良川国際会議場)
• [Presentation] BMP誘導性異所性骨における破骨細胞の役割
  • Author(s): 中村美どり
  • Organizer: 第51回日本小児歯科学会
  • Place of Presentation: 岐阜(長良川国際会議場)
• [Presentation] Oligonol(R)のStreptococcus mutansおよびPorphyromonas gingivalisに対する薬理作用の検討。
  • Author(s): 中村浩志 他
  • Organizer: 第50回日本小児歯科学会大会
  • Place of Presentation: 東京国際フォーラム（東京）