Interaction between S1P and DAMPs in periodontal inflammation

• Project/Area Number: 23593057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Osaka University
• Principal Investigator: YANAGITA Manabu 大阪大学, 歯学研究科(研究院), 助教 (80379081)
• Co-Investigator(Kenkyū-buntansha): YAMADA Satoru 大阪大学, 歯学部附属病院, 講師 (40359849)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 歯周病 / リゾリン脂質 / ダメージ関連分子パターン

Research Abstract

In this study we have investigated the effects of S1P, bioactive lipid mediator, on inflammatory responses of gingival epithelial cells. S1P receptors, S1P2 and S1P3, are expressed in gingival epithelial cells. S1P increased to produce IL-8 from gingival epithelial cells in a dose-dependent manner. S1P-induced IL-8 production upregulated synergistically in the presence of HMGB-1 or Poly(I:C). S1P-induced IL-8 induction was suppressed in S1P2/S1P3 knockdown gingival epithelial cells. These findings suggest that S1P induce inflammation in gingival epithelial cells via S1P2/S1P3.

Research Products

• [Journal Article] Cooperative effects of FGF-2 and VEGF-A in periodontal ligament cells (2014)
  • Author(s): Yanagita M, Kojima Y, Kubota M, Mori K, Yamashita M, Yamada S, Kitamura M, Murakami S
  • Journal Title: Journal of Dental Research Volume: 93 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Cooperative effects of FGF-2 and VEGF-A in periodontal ligament cells (2014)
  • Author(s): Yanagita M, Kojima Y, Kubota M, Mori K, Yamashita M, Yamada S, Kitamura M, Murakami S.
  • Journal Title: Journal of Dental Research Volume: 93 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Periodontal Regeneration and FGF-2 (2013)
  • Author(s): Kojima Y, Yanagita M, Yamada S, Kitamura M and Murakami S
  • Journal Title: Inflammation and Regeneration Volume: 33 Pages: 72-77
  • NAID: 130004943834
  • Peer Reviewed
• [Journal Article] Nicotine modulates the immunological function of dendritic cells through PPAR-gamma expression (2012)
  • Author(s): Yanagita M, Kobayashi R, Kojima Y, K Mori and Murakami S
  • Journal Title: Cellular Immunology Volume: 274 Pages: 26-33
  • Peer Reviewed
• [Journal Article] Nicotine upregulates IL-8 serection in human gingival epithelial cells following stimulation with IL-1beta or P. gingivalis lipopolysaccharide via nicotinic acetylcholine receptor signaling (2012)
  • Author(s): Kashiwagi Y, Yanagita M, Kojima Y, Shimabukuro Y and Murakami S
  • Journal Title: Archives of Oral Biology Volume: 57 Pages: 483-490
  • Peer Reviewed
• [Journal Article] Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells (2012)
  • Author(s): Iwayama T, Yanagita M, Mori K, Ozasa M, Sawada K, Kubota M, Miki K, Kojima Y, Takedachi M, Kitamura M, Shimabukuro Y, Hashikawa T and Murakami S
  • Journal Title: Journal of Periodontal Research Volume: 47 Pages: 563-571
  • Peer Reviewed
• [Journal Article] Immunomodulation of Dendritic Cells Differentiated in the Presence of Nicotine with Lipopolysaccharide From Porphyromonas gingivalis (2012)
  • Author(s): Yanagita M, Mori K, Kobayashi R, Kojima Y, Miki K, Kubota M, Yamada S, Kitamura M and Murakami S
  • Journal Title: European Journal of Oral Sciences Volume: 120 Pages: 408-414
  • Peer Reviewed
• [Journal Article] Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells. (2012)
  • Author(s): Iwayama T, Yanagita M, Mori K, Sawada K, Kubota M, Miki K, Kojima Y, Takedachi M, Kitamura M, Shimabukuro Y, Hashikawa T and Murakami S
  • Journal Title: Journal of Periodontal Research Volume: 47 Pages: 565-571
  • Peer Reviewed
• [Journal Article] Immunomodulation of dendritic cells differentiated in the presence of nicotine with lipopolysaccharide from Porphyromonas gingival is. (2012)
  • Author(s): Yanagita M, Mori K, Kobayashi R, Kojima Y, Kubota M, Miki K, Yamada S, Kitamura M and Murakami S
  • Journal Title: European Journal of Oral Sciences Volume: 120 Pages: 408-414
  • Peer Reviewed
• [Journal Article] Role of ferritin in the cytodifferentiation of periodontal ligament cells (2012)
  • Author(s): Hou J, Yamada S, Kajikawa T, Ozaki N, Awata T, Yamaba S, Murakami S
  • Journal Title: Biochem Biophys Res Commun Volume: 426 Issue: 4 Pages: 643-648
  • DOI: 10.1016/j.bbrc.2012.09.008
  • Peer Reviewed
• [Journal Article] Nicotine upregulates IL-8 serection in human gingival epithelial cells following stimulation with IL-1β or P. gingivalis lipopolysaccharide via nicotinic acetylcholine receptor signaling. (2012)
  • Author(s): Kashiwagi Y, Yanagita M*, Kojima Y, Shimabukuro Y and Murakami S
  • Journal Title: Archives of Oral Biology Volume: 57 Issue: 5 Pages: 483-490
  • DOI: 10.1016/j.archoralbio.2011.10.007
  • Peer Reviewed
• [Journal Article] Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells (2012)
  • Author(s): T.Iwayama, M.Yanagita, K.Mori, K.Sawada, M.Ozasa, M.Kubota, K.Miki, Y.Kojima, M.Takedachi, M.Kitamura, Y.Shimabukuro, T.Hashikawa, S.Murakami
  • Journal Title: J.Periodont.Res. Volume: (印刷中) Issue: 5 Pages: 563-571
  • DOI: 10.1111/j.1600-0765.2012.01467.x
  • Peer Reviewed
• [Journal Article] Nicotine modulates the immunological function of dendritic cells through PPAR-γ expression. (2012)
  • Author(s): Yanagita M, Kobayashi R, Kojima Y, K Mori and Murakami S.
  • Journal Title: Cellular Immunology Volume: 274 Issue: 1-2 Pages: 26-33
  • DOI: 10.1016/j.cellimm.2012.02.007
  • Peer Reviewed
• [Journal Article] ニコチンによる樹状細胞の機能修飾 (2012)
  • Author(s): 柳田学、森健太、村上伸也
  • Journal Title: 臨床免疫・アレルギー科 Volume: 45 Pages: 249-253
• [Journal Article] Fibroblast growth factor-2 stimulates directed migration of periodontal ligament cells via PI3-K/akt signaling and CD44/hyaluronan interaction (2011)
  • Author(s): Shimabukuro Y, Terashima H, Takedachi M, Maeda K, Nakamura T, Sawada K, Kobashi M, Awata T, Oohara H, Kawahara T, Iwayama T, Hashikawa T, Yanagita M, Yamada S and Murakami S
  • Journal Title: Journal of Cellular Physiology Volume: 226 Issue: 3 Pages: 809-821
  • DOI: 10.1002/jcp.22406
  • Peer Reviewed
• [Journal Article] Periodontal disease in a Prader-Willi syndrome patient. (2011)
  • Author(s): Yanagita M, Hirano H, Kobashi M, Nozaki T, Yamada S, Kitamura M and Murakami S
  • Journal Title: Journal of Medical Case Reports Volume: 5 Issue: 1 Pages: 329-329
  • DOI: 10.1186/1752-1947-5-329
  • Peer Reviewed
• [Journal Article] Osteoinductive and anti-inflammatory effect of royal jelly on periodontal ligament cells. (2011)
  • Author(s): Yanagita M, Kojima Y, Mori K, Yamada S and Murakami S
  • Journal Title: Biomedical Research Volume: 32 Pages: 285-291
  • NAID: 130004903826
  • Peer Reviewed
• [Presentation] 塩化カルプロニウムが歯根膜細胞および血管内皮細胞に及ぼす影響 (2013)
  • Author(s): 三木康史、柳田学、その他
  • Organizer: 第56回秋季日本歯周病学会
  • Place of Presentation: 前橋
  • Year and Date: 2013-09-22
• [Presentation] DAMPs possibly trigger inflammatory responses in human gingival epithelial cells (2013)
  • Author(s): Mori K, Yanagita M, Kubota M, Yamaba S, Yamashita M, Yamada S, Kitamura M, Murakami S
  • Organizer: 第二回国際歯科研究学会アジア太平洋地区学術大会
  • Place of Presentation: バンコク市、タイ
• [Presentation] Carpronium chloride promotes the calcification of periodontal ligament cells (2013)
  • Author(s): Miki K, Yanagita M, Mori K, Kubota M, Yamashita M, Yamada S, Kitamura M, Murakami S.
  • Organizer: 第二回国際歯科研究学会アジア太平洋地区学術大会
  • Place of Presentation: バンコク市、タイ
• [Presentation] Immunological properties of murine periodontal ligament cells (2012)
  • Author(s): Mori K, Yanagita M, Kubota M, Kojima Y, Yamaba S, Yamada S, Kitamura M, Murakami S
  • Organizer: 第60回国際歯科研究学会日本部会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2012-12-14
• [Presentation] FGF-2刺激による歯根膜細胞からのCXCR4の誘導 (2012)
  • Author(s): 久保田実木子、柳田学、その他
  • Organizer: 第137回秋季日本歯科保存学会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-22
• [Presentation] スフィンゴシン−1−リン酸がヒト歯肉上皮細胞の炎症反応に及ぼす影響 (2012)
  • Author(s): 三木康史、柳田学、その他
  • Organizer: 第136回春季日本歯科保存学会
  • Place of Presentation: 宜野湾
  • Year and Date: 2012-06-29
• [Presentation] FGF-2 induces VEGF expression by periodontal ligament cells (2012)
  • Author(s): Kojima Y, Yanagita M,その他
  • Organizer: 90th IADR
  • Place of Presentation: Iguacu Falls, Brazil
• [Presentation] FGF-2 induces VEGF expression by periodontal ligament cells (2012)
  • Author(s): Kojima Y, Yanagita M, Mori K, Murakami S
  • Organizer: 98^<th> American Academy of Periodontology Annual Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] Cooperative effects of FGF-2 and VEGF in periodontal ligament cells (2011)
  • Author(s): Kojima Y, Yanagita M, Mori K, Yamashita M, Nozaki T, Yamada S,その他
  • Organizer: 第59回国際歯科研究学会日本部会学術大会
  • Place of Presentation: 広島
  • Year and Date: 2011-10-08
• [Presentation] スフィンゴシン−1−リン酸がヒト歯肉線維芽細胞の炎症反応に及ぼす影響 (2011)
  • Author(s): 森健太、柳田学、その他
  • Organizer: 第54回日本歯周病学会秋季学術大会
  • Place of Presentation: 下関
  • Year and Date: 2011-09-24
• [Presentation] TLR2/TLR3共刺激が歯肉上皮細胞の免疫応答に及ぼす影響 (2011)
  • Author(s): 森健太、柳田学、児嶋由子、中村友美、山下元三、山田聡、その他
  • Organizer: 第134回春季日本歯科保存学会
  • Place of Presentation: 千葉
  • Year and Date: 2011-06-08
• [Presentation] FGF-2により誘導される血管新生への歯根膜細胞の関与 (2011)
  • Author(s): 児嶋由子、柳田学、森健太、山下元三、山田聡、その他
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-03
• [Presentation] FGF-2により誘導される血管新生へのマウス歯根膜細胞の関与 (2011)
  • Author(s): 児嶋由子、柳田学、森健太、野崎剛徳、山田聡、その他
  • Organizer: 第54回日本歯周病学会春季学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-28
• [Presentation] ヒト歯根膜細胞におけるインフラマゾームの発現 (2011)
  • Author(s): 山羽聡子、山田聡、梶川哲宏、尾崎亘弘、粟田敏仁、柳田学、その他
  • Organizer: 第55回日本歯周病学会春季学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-18
• [Presentation] TLR2/TLR3共刺激が歯肉上皮細胞の免疫応答に及ぼす影響 (2011)
  • Author(s): 森健太、柳田学、児嶋由子、中村友美、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第134回春季日本歯科保存学会
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート（ 千葉県 ）
• [Presentation] スフィンゴシン－１－リン酸がヒト歯肉線維芽細胞の炎症反応に及ぼす影響 (2011)
  • Author(s): 森健太、柳田学、児嶋由子、久保田実木子、山田聡、北村正博、村上伸也
  • Organizer: 第54回日本歯周病学会秋季学術大会
  • Place of Presentation: 海峡メッセ下関（ 山口県 ）
• [Presentation] ヒト歯根膜細胞の老化により誘導されるSASPの解析
  • Author(s): 池上久仁子、山下元三、山羽聡子、中村友美、森健太、粟田敏仁、柳田学、北村正博、村上伸也
  • Organizer: 第138回秋季日本歯科保存学会
  • Place of Presentation: 福岡市、福岡県
• [Presentation] 塩化カルプロニウムが歯根膜細胞および血管内皮細胞に及ぼす影響
  • Author(s): 三木康史、柳田学、伊山舜吉、森健太、久保田実木子、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第56回秋季日本歯周病学会
  • Place of Presentation: 前橋市、群馬県
• [Presentation] イムノクロマト法を用いた歯肉浸出液中のカルプロテクチン測定による歯周病診断
  • Author(s): 木戸淳一、永田俊彦、多部田康一、山崎和久、吉江弘正、渡辺久、和泉雄一、須田玲子、山本松男、柴秀樹、栗原英見、柳田学、北村正博、南部敏之、水野光春、村上伸也
  • Organizer: 第56回秋季日本歯周病学会
  • Place of Presentation: 前橋市、群馬県
• [Presentation] 歯根膜特異的分子PLAP-1による炎症制御機構解明
  • Author(s): 山羽聡子、山田聡、粟田敏仁、阪下裕美、津島賢一朗、梶川哲宏、柳田学、北村正博、村上伸也
  • Organizer: 第139回秋季日本歯科保存学会
  • Place of Presentation: 秋田市、秋田県
• [Presentation] スフィンゴシン－１－リン酸がヒト歯肉上皮細胞の炎症反応に及ぼす影響
  • Author(s): 三木康史、柳田学、森健太、久保田実木子、伊山舜吉、竹立匡秀、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第136回春季日本歯科保存学会
  • Place of Presentation: 宜野湾市、沖縄県
• [Presentation] Immunological properties of murine periodontal ligament cells
  • Author(s): Mori K, Yanagita M, Kubota M, Kojima Y, Yamaba S, Yamada S, Kitamura M, Murakami S
  • Organizer: 第60回国際歯科研究学会日本部会学術大会
  • Place of Presentation: 新潟市、新潟県
• [Presentation] Expression analysis of inflammasome in periodontal tissues
  • Author(s): Yamaba S, Yamada S, Kajikawa T, Ozaki N, Awata H, Sakashita H, Yanagita M, Kitamura M, Murakami S
  • Organizer: 第60回国際歯科研究学会日本部会学術大会
  • Place of Presentation: 新潟市、新潟県
• [Book] ザ・ペリオドントロジー 第二版 (2014)
  • Author(s): 柳田学、村上伸也 （編集主幹：和泉雄一、木下淳博、沼部幸博、山本松男）
  • Total Pages: 279
  • Publisher: 永末書店
• [Book] 炎症性メディエーターと歯周疾患の関係;ザ・ペリオドントロジー第二版 (2013)
  • Author(s): 柳田学、村上伸也
  • Publisher: 永末書店