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The effect of IL-22 on biological activity of periodontal ligament cells

Research Project

Project/Area Number 23593076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Periodontal dentistry
Research InstitutionHyogo Medical University

Principal Investigator

OHYAMA hideki  兵庫医科大学, 医学部, 准教授 (90280685)

Co-Investigator(Kenkyū-buntansha) TERADA Nobuyuki  兵庫医科大学, 医学部, 名誉教授 (50150339)
NAKASHO Keiji  兵庫医科大学, 医学部, 教授 (00217712)
YAMADA Naoko  兵庫医科大学, 医学部, 講師 (10319858)
YAMANEGI Koji  兵庫医科大学, 医学部, 講師 (00434944)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords歯根膜細胞 / IL-22 / 石灰化誘導 / 歯根膜由来細胞 / IL-22 / ヒト歯根膜由来細胞
Research Abstract

This study focused on the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. We found that IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. In addition, the production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner.IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (11 results) Book (1 results)

  • [Journal Article] Decreased expression of VE-cadherin and claudin-5 and increased phosphorylation of VE-cadherin in vascular endothelium in nasal polyps.2013

    • Author(s)
      Yukitatsu Y, Hata M, Yamanegi K, Yamada N, Ohyama H, Nakasho K, Kojima Y, Oka H, Tsuzuki K, Sakagami M, Terada N.
    • Journal Title

      Cell Tissue Res

      Volume: 352 Issue: 3 Pages: 647-657

    • DOI

      10.1007/s00441-013-1583-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] T-cell Responses Involved in the Predisposition to Periodontal Disease: Lessons from Immunogenetic Studies of Leprosy2013

    • Author(s)
      Hideki Ohyama, Nahoko Kato-Kogoe, Kazu Takeuchi-Hatanaka, Koji Yamanegi, Naoko Yamada, Keiji Nakasho, Sho Matsushita and Nobuyuki Terada
    • Journal Title

      J Clin Cell Immunol

      Volume: S1 Pages: 1-7

    • DOI

      10.4172/2155-9899.s1-005

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The promotional effect of IL-22 on mineralization activity of periodontal ligament cells2012

    • Author(s)
      Kato-Kogoe N, Nishioka T, Kawabe M, Kataoka F, Yamanegi K, Yamada N, Hata M, Yamamoto T, Nakasho K, Urade M, Terada N, Ohyama H
    • Journal Title

      Cytokine

      Volume: 59(1) Issue: 1 Pages: 41-8

    • DOI

      10.1016/j.cyto.2012.03.024

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma2012

    • Author(s)
      Yamanegi K, Yamane J, Kobayashi K, Kato-Kogoe N, Ohyama H, Nakasho K, YamadaN, Hata M, Fukunaga S, Futani H, Okamura H, Terada N.
    • Journal Title

      cells to Fas- and NK cell-mediated cell death.Int J Oncol.

      Volume: 41(1) Pages: 83-91

    • DOI

      10.3892/ijo.2012.1438

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Downregulation of matrix metalloproteinase-9 mRNA by valproic acid plays a role in inhibiting the shedding of MHC class I-related molecules A and B on the surface of human osteosarcoma cells.2012

    • Author(s)
      Yamanegi K, Yamane J, Kobayashi K, Ohyama H, Nakasho K, Yamada N, Hata M, Fukunaga S, Futani H, Okamura H, Terada N.
    • Journal Title

      Oncol Rep.

      Volume: 28(5) Issue: 5 Pages: 1585-1590

    • DOI

      10.3892/or.2012.1981

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypoxia downregulates the expression of cell surface MICA without increasing soluble MICA in osteosarcoma cells in a HIF-1α-dependent manner.2012

    • Author(s)
      Yamada N, Yamanegi K, Ohyama H, Hata M, Nakasho K, Futani H, Okamura H, Terada N.
    • Journal Title

      Int J Oncol.

      Volume: 41(6) Issue: 6 Pages: 2005-2012

    • DOI

      10.3892/ijo.2012.1630

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The promotional effect of IL-22 on mineralization activity of periodontal ligament cells.2012

    • Author(s)
      Nahoko Kato-Kogoe
    • Journal Title

      Cytokine

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Adipocyte-macrophage interaction may mediate LPS-induced low-grade inflammation : Potential link with metabolic complications2011

    • Author(s)
      Nakarai, H, Yamashita, A, Nagayasu, S, Iwashita, M, Kumamoto, S, Ohyama, H, Hata, M, Soga, Y, Kushiyama, A, Asano, T, Abiko, Y, Nishimura, F
    • Journal Title

      Innate Immunity

      Volume: 18 Issue: 1 Pages: 164-170

    • DOI

      10.1177/1753425910393370

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] IL-22/TNF-αの単独および共刺激によって誘導されるヒト歯根膜由来細胞の生物学的活性2013

    • Author(s)
      川辺睦記,浦出雅裕,大山秀樹
    • Organizer
      第67回日本口腔科学会学術集会
    • Place of Presentation
      栃木県総合文化センター(栃木県)
    • Year and Date
      2013-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ステロイドを長期服用する関節リウマチ患者に発症した肺クリプトコックス症の1剖検例2013

    • Author(s)
      大山秀樹
    • Organizer
      真菌症フォーラム第14回学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Th17細胞産生性サイトカインが血管石灰化に及ぼす影響2012

    • Author(s)
      大山秀樹
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2012-04-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Th17細胞産生性サイトカインが血管石灰化に及ぼす影響2012

    • Author(s)
      大山秀樹
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 心臓血管外科疾患周術期患者の口腔衛生管理に関する統計学的解析2012

    • Author(s)
      木﨑久美子
    • Organizer
      第55回春季日本歯周病学会学術大会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] IL-22がヒト歯根膜由来細胞の石灰化ノジュール形成に及ぼす影響2011

    • Author(s)
      大山秀樹
    • Organizer
      第54回日本歯周病学会春季学術大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2011-05-28
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-22存在下においてヒト歯根膜由来細胞が発現する遺伝子の網羅的解析2011

    • Author(s)
      川辺睦記
    • Organizer
      第54回日本歯周病学会春季学術大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2011-05-27
    • Related Report
      2013 Final Research Report
  • [Presentation] IL12BR2転写活性の個体差におけるAP-1ファミリー転写因子の関与.2011

    • Author(s)
      大山秀樹
    • Organizer
      第100回日本病理学会総会
    • Place of Presentation
      パシィフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] IL-22がヒト歯根膜由来細胞の石灰化ノジュール形成に及ぼす影響2011

    • Author(s)
      大山秀樹
    • Organizer
      第54回日本歯周病学会春季学術大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] IL-22存在下においてヒト歯根膜由来細胞が発現する遺伝子の網羅的解析2011

    • Author(s)
      川辺睦記
    • Organizer
      第54回日本歯周病学会春季学術大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] IL-22/TNF-αの単独および共刺激によって誘導されるヒト歯根膜由来細胞の生物学的活性

    • Author(s)
      川邊睦記,浦出雅裕,大山秀樹
    • Organizer
      第67回 NPO法人 日本口腔科学会学術集会
    • Place of Presentation
      宇都宮
    • Related Report
      2013 Annual Research Report
  • [Book] Leprosy-Science working towards dignity2011

    • Author(s)
      Hideki Ohyama
    • Publisher
      Tokai University Press
    • Related Report
      2013 Final Research Report 2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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