Mechanisms of spindle elongation controlling the chromosome segregation during anaphase: quantitative measurement of forces generated by the mitotic spindle

• Project/Area Number: 23612006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Mechanobiology
• Research Institution: Waseda University
• Principal Investigator: ITABASHI Takeshi 早稲田大学, 理工学術院, 講師 (20434384)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 細胞骨格 / 紡錘体 / 染色体分配 / 細胞分裂

Research Abstract

The process of partitioning chromosomes into daughter cells during cell division is mediated by a bipolar spindle of proper size and shape. We planned to investigate the underlying mechanisms of spindle elongation machinery by quantitative force measurements. The main results of this project are summarized as follows: (1) We developed the technique for real-time observation to analyze dynamics of chromokinesin within meiotic spindle. (2) Using micromanipulation technique, we found the parameters controlling the size of vertebrate meiotic spindle. (3) We revealed the micromechanics of meiotic spindle along the pole-to-pole axis. (4) We succeeded in quantitatively measuring forces generated by anaphase cells.

Research Products

• [Journal Article] Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis (2014)
  • Author(s): Takagi, J., Itabashi, T., Suzuki, K., Shimamoto, Y., Kapoor, TM., and Ishiwata S.
  • Journal Title: Biophys. J. Volume: 106 Issue: 3 Pages: 735-740
  • DOI: 10.1016/j.bpj.2013.12.033
  • Peer Reviewed
• [Journal Article] Using micromanipulation to analyze control of vertebrate meiotic spindle size (2013)
  • Author(s): Takagi, J., Itabashi, T., Suzuki, K., Kapoor, T.M., Shimamoto, Y., and Ishiwata, S.
  • Journal Title: Cell Rep. Volume: 5 Issue: 1 Pages: 44-50
  • DOI: 10.1016/j.celrep.2013.09.021
  • Peer Reviewed
• [Journal Article] Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array (2013)
  • Author(s): Takagi, J., Itabashi, T., Suzuki, K., and Ishiwata, S.
  • Journal Title: Sci. Rep. Volume: 3 Issue: 1 Pages: 2808-2808
  • DOI: 10.1038/srep02808
  • Peer Reviewed
• [Journal Article] Responses of chromosome segregation machinery to mechanical perturbations (2013)
  • Author(s): Itabashi, T., Takagi, J., Suzuki, K., and Ishiwata, S.
  • Journal Title: BIOPHYSICS Volume: 9 Issue: 0 Pages: 73-78
  • DOI: 10.2142/biophysics.9.73
  • NAID: 130003366032
  • ISSN: 1349-2942
  • Peer Reviewed
• [Journal Article] 染色体分配装置の構造と機能の力学応答 (2013)
  • Author(s): 板橋岳志,石渡信一
  • Journal Title: 実験医学増刊号 Volume: 31(2) Pages: 193-198
• [Journal Article] 染色体分配装置の構造と機能の力学応答 (2013)
  • Author(s): 板橋岳志, 石渡信一
  • Journal Title: 実験医学 増刊号 Volume: 31 Pages: 63-68
• [Journal Article] Mechanical Impulses Control Anaphase Onset in a Mammalian Cell (2012)
  • Author(s): 板橋岳志, 石渡信一
  • Journal Title: Seibutsu Butsuri Volume: 52 Issue: 5 Pages: 239-240
  • DOI: 10.2142/biophys.52.239
  • NAID: 10030877037
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] Mechanical impulses can control metaphase progression in a mammalian cell (2012)
  • Author(s): Itabashi, T., et al.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 109 Issue: 19 Pages: 7320-7325
  • DOI: 10.1073/pnas.1116749109
  • Peer Reviewed
• [Presentation] Using micromanipulation to analyze control of cell division machinery (2014)
  • Author(s): Takeshi Itabashi, Jun Takagi and Shin'ichi Ishiwata
  • Organizer: International Symposium on the Diversity of Cell Division System in Eukaryotes
  • Place of Presentation: 東京
  • Year and Date: 2014-03-25
• [Presentation] 紡錘体の構造ダイナミクスを探る (2013)
  • Author(s): 板橋岳志,高木潤,鈴木和也,石渡信一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-06
• [Presentation] 顕微操作によってわかる紡錘体の力学特性 (2013)
  • Author(s): 板橋岳志,石渡信一.
  • Organizer: 日本動物学会関東支部第65回大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-16
• [Presentation] 有糸分裂中期に観察される染色体振動の解析 (2013)
  • Author(s): 中山馨太、高木潤、板橋岳志、石渡信一
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
• [Presentation] Symmetrical shape of the meiotic spindle is dynamically balanced (2013)
  • Author(s): Kazuya Suzuki, Jun Takagi, Takeshi Itabashi, Shin’ichi Ishiwata
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] 中期紡錘体の形状制御メカニズムの解明 (2013)
  • Author(s): 高木潤, 板橋岳志, 鈴木和也, 島本勇太, Tarun M. Kapoor, 石渡信一
  • Organizer: 2013年生体運動研究合同班会議
  • Place of Presentation: 広島
• [Presentation] 紡錘体の力学応答 (2012)
  • Author(s): 板橋岳志
  • Organizer: 第2回分子モーター討論会
  • Place of Presentation: 東京
  • Year and Date: 2012-06-07
• [Presentation] 紡錘体は一方の極から他方へ構造変化を伝搬することで対称形状を維持する (2012)
  • Author(s): 鈴木和也, 高木潤, 板橋岳志, 石渡信一
  • Organizer: 第50回 日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 染色体分配機構を司る力のメカノケミカル制御機構を探る (2012)
  • Author(s): 板橋岳志
  • Organizer: 細胞増殖制御終了シンポジウム
  • Place of Presentation: 東京
  • Invited
• [Presentation] 細胞分裂を司る力のメカノケミカル制御機構を探る (2011)
  • Author(s): 板橋岳志、石渡信一
  • Organizer: 第84回日本生化学会大会(招待講演)
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] 顕微操作によってわかる紡錘体の力学特性
  • Author(s): 板橋岳志, 石渡信一
  • Organizer: 日本動物学会関東支部第65回大会
  • Place of Presentation: 東京
  • Invited
• [Remarks] 雑誌論文②についてのプレスリリース
  • URL: http://www.waseda.jp/jp/news13/131014_ishiwata.html
• [Remarks] 雑誌論文③についてのプレスリリース
  • URL: http://www.waseda.jp/jp/news13/131001_chromosome.html
• [Remarks] 紡錘体の大きさを決めるパラメータの導出に成功
  • URL: http://www.waseda.jp/jp/news13/131014_ishiwata.html
• [Remarks] 染色体を輸送する分子モーターの運動を紡錘体内で捉えることに成功
  • URL: http://www.waseda.jp/jp/news13/131001_chromosome.html
• [Remarks] 理工・石渡研究室、外力で細胞分裂を制御できることを発見
  • URL: http://www.waseda.jp/jp/news12/120417_cellcontrol.html
• [Remarks] 外力で細胞分裂を制御できることを発見
  • URL: http://www.yomiuri.co.jp/adv/wol/campus/newreport_1208_02.htm