Mechanisms of spindle elongation controlling the chromosome segregation during anaphase: quantitative measurement of forces generated by the mitotic spindle
Project/Area Number |
23612006
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Mechanobiology
|
Research Institution | Waseda University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | 細胞骨格 / 紡錘体 / 染色体分配 / 細胞分裂 |
Research Abstract |
The process of partitioning chromosomes into daughter cells during cell division is mediated by a bipolar spindle of proper size and shape. We planned to investigate the underlying mechanisms of spindle elongation machinery by quantitative force measurements. The main results of this project are summarized as follows: (1) We developed the technique for real-time observation to analyze dynamics of chromokinesin within meiotic spindle. (2) Using micromanipulation technique, we found the parameters controlling the size of vertebrate meiotic spindle. (3) We revealed the micromechanics of meiotic spindle along the pole-to-pole axis. (4) We succeeded in quantitatively measuring forces generated by anaphase cells.
|
Report
(4 results)
Research Products
(25 results)
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[Journal Article] Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis2014
Author(s)
Takagi, J., Itabashi, T., Suzuki, K., Shimamoto, Y., Kapoor, TM., and Ishiwata S.
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Journal Title
Biophys. J.
Volume: 106
Issue: 3
Pages: 735-740
DOI
Related Report
Peer Reviewed
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[Journal Article] Using micromanipulation to analyze control of vertebrate meiotic spindle size2013
Author(s)
Takagi, J., Itabashi, T., Suzuki, K., Kapoor, T.M., Shimamoto, Y., and Ishiwata, S.
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Journal Title
Cell Rep.
Volume: 5
Issue: 1
Pages: 44-50
DOI
Related Report
Peer Reviewed
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