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Development of novel screening method based on the visualization of permeability distribution of cell layer

Research Project

Project/Area Number 23615001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Reliable environmental measurement methods
Research InstitutionTohoku University

Principal Investigator

MIYAMOTO Koichiro  東北大学, 工学(系)研究科(研究院), 准教授 (70447142)

Co-Investigator(Kenkyū-buntansha) HAN Jyunkyu  筑波大学, 生命環境科学研究科, 准教授 (40455928)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords化学センサ / タイトジャンクション / スクリーニング / 細胞アッセイ / 化学イメージセンサ / 安全環境計測法 / 化学イメージング
Research Abstract

Chemical image sensor is a sensor which can visualize the ion distribution in the electrolyte. In this study, a portable chemical imaging system which is small, inexpensive and working stand-alone was developed. In addition to that, the visualization of resistivity distribution of cultured-cell layer was succeeded. It indicates that the visualization of the tight-junction based on electrical property will be possible in the future. Based on this result, novel screening assay to evaluate the influence of chemicals to the cells can be also expected.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results) (of which Invited: 4 results) Remarks (1 results)

  • [Journal Article] Genistein-induced G2/M cell cycle arrest of human intestinal colon cancer Caco-2 cells is associated with Cyclin B1 and Chk2 down-regulation2013

    • Author(s)
      Junkyu Han, Yui Kurita, Hiroko Isoda
    • Journal Title

      Cytotechnology

      Volume: 65巻 Issue: 6 Pages: 973-978

    • DOI

      10.1007/s10616-013-9592-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Reversible Increase in Tight Junction Permeability Induced by Capsaicin is Mediated via Cofilin-Actin Cytoskeletal Dynamics and Decreased Level of Occludin2013

    • Author(s)
      Tomoko Shiobara, Takeo Usui, Junkyu Han, Hiroko Isoda, Yoko Nagumo
    • Journal Title

      PLOS ONE

      Volume: 8巻 Issue: 11 Pages: e79954-e79954

    • DOI

      10.1371/journal.pone.0079954

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of enzymatic reaction in a microfluidic channel using chemical imaging sensor2013

    • Author(s)
      Ko-ichiro Miyamoto, Yuji Hirayama, Torsten Wagner, Michael J. Schöning, Tatsuo Yoshinobu
    • Journal Title

      Electrochimica Acta

      Volume: 113巻 Pages: 768-772

    • DOI

      10.1016/j.electacta.2013.08.089

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] High speed and high resolution chemical imaging based on a new type of OLED-LAPS set-up2012

    • Author(s)
      Carl Frederik Werner, Torsten Wagner, Ko-ichiro Miyamoto, Tatsuo Yoshinobu, Michael J. Schöning
    • Journal Title

      Sensors and Actuators B:Chemical

      Volume: 175巻 Pages: 118-122

    • DOI

      10.1016/j.snb.2011.12.102

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 小型化学イメージセンサヘッドの開発と大腸菌コロニー検出への応用2014

    • Author(s)
      佐藤舞子, 宮本浩一郎, 吉信達夫
    • Organizer
      第56回化学センサ研究発表会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] OLED-based and embedded sensor platform for chemical imaging2014

    • Author(s)
      Carl Frederik Werner, Tatsuo Yoshinobu, Ko-ichiro Miyamoto, Michael J. Schöning, Torsten Wagner
    • Organizer
      IMCS 2014
    • Place of Presentation
      アルゼンチン,ブエノスアイレス
    • Related Report
      2013 Final Research Report
  • [Presentation] 化学イメージセンサを用いた細胞層の抵抗分布測定に関する検討2013

    • Author(s)
      于冰, 宮本浩一郎, 吉信達夫
    • Organizer
      第68回応用物理学会東北支部学術講演会
    • Place of Presentation
      山形
    • Related Report
      2013 Final Research Report
  • [Presentation] 小腸培養細胞株を用いた多チャンネルTER測定とタイトジャンクション可視化への取り組み2013

    • Author(s)
      宮本浩一郎
    • Organizer
      第26回日本動物細胞工学会2013年度大会
    • Place of Presentation
      福井
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 半導体化学イメージセンサ~近年の進展とその応用例~2013

    • Author(s)
      宮本浩一郎, 吉信達夫
    • Organizer
      日本鉄鋼協会第165回春季講演大会「革新的水素侵入抑制表面の構築にむけて」シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 「化学イメージセンサを用いた細胞層の抵抗分布測定に関する検討」2013

    • Author(s)
      于冰, 宮本浩一郎, 吉信達夫
    • Organizer
      第68回応用物理学会東北支部学術講演会
    • Place of Presentation
      山形大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 「小腸培養細胞株を用いた多チャンネルTER測定と タイトジャンクション可視化 への取り組み」2013

    • Author(s)
      宮本浩一郎
    • Organizer
      第26回日本動物細胞工学会2013年度大会
    • Place of Presentation
      福井県福井市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 「半導体化学イメージセンサ ~近年の進展とその応用例~」2013

    • Author(s)
      宮本浩一郎、吉信達夫
    • Organizer
      日本鉄鋼協会 第165回春季講演大会 「革新的水素侵入抑制表面の構築にむけて」シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Development of the system for multi-channel TER measurement2012

    • Author(s)
      K. Miyamoto, J. Han, T. Yoshinobu, H. Isoda
    • Organizer
      The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
    • Place of Presentation
      名古屋
    • Year and Date
      2012-11-27
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Position Control of Chemical Reaction in a LAPS-Microfluidic System2012

    • Author(s)
      Y. Hirayama, K. Miyamoto, T. Wagner, T. Yoshinobu
    • Organizer
      6th International Symposium on Medical, Bio- and Nano-Electronics
    • Place of Presentation
      宮城
    • Year and Date
      2012-03-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of Stand- Alone Chemical Imaging System and its Application to Microorganisms2012

    • Author(s)
      A. Matsuo, K. Miyamoto, T. Wagner, T. Yoshinobu
    • Organizer
      6th International Symposium on Medical, Bio- and Nano-Electronics
    • Place of Presentation
      宮城
    • Year and Date
      2012-03-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Visualization of enzymatic reaction in a microfluidic channel using chemical imaging sensor2012

    • Author(s)
      K. Miyamoto, Y. Hirayama, T. Wagner, T. Yoshinobu, M. J. Schöning
    • Organizer
      9th International Symposium on Electrochemical Micro & Nanosystem Technology (EMNT 2012)
    • Place of Presentation
      オーストリア,リンツ
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of Stand-Alone Chemical Imaging System and its Application to Microorganisms2012

    • Author(s)
      A. Matsuo, K. Miyamoto, T. Wagner and T. Yoshinobu
    • Organizer
      6th International Symposium on Medical, Bio- and Nano-Electronics
    • Place of Presentation
      宮城県仙台市 東北大学
    • Related Report
      2011 Research-status Report
  • [Presentation] High speed and high resolution chemical imaging based on a new type of OLED-LAPS set-up2011

    • Author(s)
      Carl Frederik Werner, Torsten Wagner, Ko-ichiro Miyamoto, Tatsuo Yoshinobu, Michael J. Schöning
    • Organizer
      Eurosensors XXV
    • Place of Presentation
      ギリシャ,アテネ
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.bme.ecei.tohoku.ac.jp/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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