Development of Reactive Pyrolysis-GC/MS Combined with Magnetic Bead Probes for Rapid Detection of Drug-Resistant Bacteria
| Project/Area Number |
23615007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Reliable environmental measurement methods
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| Research Institution | Chubu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUMIUCHI Kaname 中部大学, 応用生物学部, 准教授 (50329851)
KAWAMURA Kumiko 名古屋大学, 医学部, 准教授 (30335054)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 薬剤耐性菌 / 迅速検出 / 反応熱分解 / 抗体磁性ビーズ / プローブ / 磁性粒子プローブ / 薬剤耐性 / 反応熱分解GC/MS / 有機アルカリ / リン脂質 / 脂肪酸 / MALDI-TOF-MS / MALDI-TOF/MS / 抗原抗体反応 / 反応熱分解GC / 加水分解及びメチル化 |
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| Research Abstract |
A new analytical tool for bacteria samples was developed by combining reactive pyrolysis gas-chromatography/mass spectrometry with magnetic bead probes. By using this method, a certain species of bacteria can be selectively captured, and the structures of their lipid components were able to be analyzed in detail. Moreover, this technique was successfully applied to rapid detection of drug-resistant bacteria in various biological samples.
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Report
(4 results)
Research Products
(51 results)
