Simultaneous Determination of Oxidized- and Reduced-thiols: Application to the Estimation of Balance of the Red/Ox State
| Project/Area Number |
23615009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Reliable environmental measurement methods
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TODOROKI Kenichiro 静岡県立大学, 薬学部, 准教授 (70341451)
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| Research Collaborator |
HAYAMA Tadashi 福岡大学, 薬学部, 助教 (90549693)
TAMASHIMA Erina 福岡大学, 大学院薬学研究科
KIYOKAWA Ena 福岡大学, 大学院薬学研究科
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | レドックス / 分析化学 / α－リポ酸 / エキシマー蛍光 / 蛍光誘導体化法 / クロマトグラフィー / 酸化ストレス / 蛍光誘導体化 / α-リポ酸 / リポ酸 |
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| Outline of Final Research Achievements |
In this research, a simultaneous determination of reduced and oxidized α-lipoic acid (LA) has been developed and was applied to the estimation of biological Red/Ox balance. This analysis method involves reversed-phase liquid chromatographic separation of LA-related compounds, on-line reduction of intramolecular disulfide structure to dithiol structure, post-column derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Balance of biological Red/Ox state were calculated from the amount of reduced-LA and that of oxidized-LA in samples. Simple, sensitive, and selective estimation of Red/Ox balance could be achieved.
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Assessment of the Efficacy of Anticancer Drugs by Amino Acid Metabolomics Using Fluorescence Derivatization-HPLC2014
Author(s)
Ryoko Tomita, Kenichiro Todoroki, Kazuyuki Machida, Sho Nishida, Hiroshi Maruoka, Hideyuki Yoshida, Toshihiro Fujioka, Manabu Nakashima, Masatoshi Yamaguchi, Hitoshi Nohta
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Journal Title
Analytical Sciences
Volume: 30
Issue: 7
Pages: 751-758
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed / Open Access
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