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Simultaneous Determination of Oxidized- and Reduced-thiols: Application to the Estimation of Balance of the Red/Ox State

Research Project

Project/Area Number 23615009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Reliable environmental measurement methods
Research InstitutionFukuoka University

Principal Investigator

YOSHIDA Hideyuki  福岡大学, 薬学部, 准教授 (20301690)

Co-Investigator(Renkei-kenkyūsha) TODOROKI Kenichiro  静岡県立大学, 薬学部, 准教授 (70341451)
Research Collaborator HAYAMA Tadashi  福岡大学, 薬学部, 助教 (90549693)
TAMASHIMA Erina  福岡大学, 大学院薬学研究科
KIYOKAWA Ena  福岡大学, 大学院薬学研究科
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsレドックス / 分析化学 / α-リポ酸 / エキシマー蛍光 / 蛍光誘導体化法 / クロマトグラフィー / 酸化ストレス / 蛍光誘導体化 / α-リポ酸 / リポ酸
Outline of Final Research Achievements

In this research, a simultaneous determination of reduced and oxidized α-lipoic acid (LA) has been developed and was applied to the estimation of biological Red/Ox balance. This analysis method involves reversed-phase liquid chromatographic separation of LA-related compounds, on-line reduction of intramolecular disulfide structure to dithiol structure, post-column derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Balance of biological Red/Ox state were calculated from the amount of reduced-LA and that of oxidized-LA in samples. Simple, sensitive, and selective estimation of Red/Ox balance could be achieved.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (26 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (14 results)

  • [Journal Article] Selective Extraction of Nucleotides with Fluorous Biphasic System Utilizing Perfluoroalkylamine as an Ion-pair Reagent2015

    • Author(s)
      Tadashi Hayama, Ena Kiyokawa, Hideyuki Yoshida, Osamu Imakyure, Masatoshi Yamaguchi, Hitoshi Nohta
    • Journal Title

      Chromatography

      Volume: 36 (1) Issue: 1 Pages: 13-18

    • DOI

      10.15583/jpchrom.2015.001

    • NAID

      130005069043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Liquid Chromatography-tandem Mass Spectrometry with Fluorous Derivatization Method for Selective Analysis of Sialyl Oligosaccharides2014

    • Author(s)
      Yohei Sakaguchi, Tadashi Hayama, Hideyuki Yoshida, Miki Itoyama, Kenichiro Todoroki, Masatoshi Yamaguchi, Hitoshi Nohta
    • Journal Title

      Rapid Communication in Mass Spectrometry

      Volume: 28 (23) Issue: 23 Pages: 2481-2489

    • DOI

      10.1002/rcm.7042

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of the Efficacy of Anticancer Drugs by Amino Acid Metabolomics Using Fluorescence Derivatization-HPLC2014

    • Author(s)
      Ryoko Tomita, Kenichiro Todoroki, Kazuyuki Machida, Sho Nishida, Hiroshi Maruoka, Hideyuki Yoshida, Toshihiro Fujioka, Manabu Nakashima, Masatoshi Yamaguchi, Hitoshi Nohta
    • Journal Title

      Analytical Sciences

      Volume: 30 Issue: 7 Pages: 751-758

    • DOI

      10.2116/analsci.30.751

    • NAID

      130004442316

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorous Affinity-based Separation Techniques for the Analysis of Biogenic and Related Molecules2014

    • Author(s)
      Tadashi Hayama, Hideyuki Yoshida, Masatoshi Yamaguchi, Hitoshi Nohta
    • Journal Title

      Journal of Pharmaceutical and Biomedical Analysis

      Volume: 101 (1) Pages: 151-160

    • DOI

      10.1016/j.jpba.2014.04.035

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] モノリス型フルオラス固相カラムを用いたパーフルオロアルキル化アミノ酸の選択的抽出とLC-MS/MS分析2014

    • Author(s)
      玉嶋江莉奈,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Journal Title

      Chromatography

      Volume: 35 (sup1) Pages: 61-62

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Concerted Derivatization and Concentration Method with Dispersive Liquid-liquid Microextraction for Liquid Chromatographic Analysis of 5-Hydroxyindoles in Human Serum2013

    • Author(s)
      Tadashi Hayama, Yurika Yabuuchi, Tomomi Iwamatsu, Erina Tamashima, Yusuke Kawami, Miki Itoyama, Hideyuki Yoshida, Masatoshi Yamaguchi, Hitoshi Nohta
    • Journal Title

      Talanta

      Volume: 117 Pages: 27-31

    • DOI

      10.1016/j.talanta.2013.08.035

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Determination of Cortisol and Cortisone in Human Saliva Using Dispersive Liquid-liquid Microextraction Coupled to HPLC Analysis2013

    • Author(s)
      玉嶋江莉奈,甲斐知美,轟木堅一郎,川見祐介,糸山美紀,巴山忠,吉田秀幸,山口政俊,能田均
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 62 Issue: 8 Pages: 719-723

    • DOI

      10.2116/bunsekikagaku.62.719

    • NAID

      10031178770

    • ISSN
      0525-1931
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] エキシマー蛍光誘導体化法の分析化学的利用2013

    • Author(s)
      吉田秀幸
    • Journal Title

      ぶんせき

      Volume: 457 (1) Pages: 6-12

    • NAID

      10031147349

    • Related Report
      2012 Research-status Report
  • [Journal Article] Liquid chromatographic determination of microcystins in water samples following pre-column excimer fluorescence derivatization with 4-(1-pyrene)butanoic acid hydrazide2012

    • Author(s)
      T. Hayama, K. Katoh, T. Aoki, M. Itoyama, K. Todoroki, H. Yoshida, M. Yamaguchi, H. Nohta
    • Journal Title

      Analytica Chimica Acta

      Volume: 755 Pages: 93-99

    • DOI

      10.1016/j.aca.2012.10.009

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] エキシマー蛍光誘導体化によるアルコール類のLC分析2012

    • Author(s)
      井上貴文,吉田秀幸,佐々木知美,坂口洋平,川見祐介,糸山美紀,巴山忠,能田均,山口政俊
    • Journal Title

      Chromatography

      Volume: 33 (sup2) Pages: 85-86

    • Related Report
      2012 Research-status Report
  • [Journal Article] Determination of Histamine in Microdialysis Samples from Guinea Pig Skin by High-performance Liquid Chromatography with Fluorescence Detection2012

    • Author(s)
      T.Yoshitake, S.Ijiri, S.Yoshitake, K.Todoroki, H.Yoshida, J.Kehr, H.Nohta, M.Yamaguchi
    • Journal Title

      Skin Pharmacology and Physiology

      Volume: 25(2) Issue: 2 Pages: 65-72

    • DOI

      10.1159/000330899

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] ペンタフルオロフェニル構造を用いる分離指向性誘導体化法の開発2011

    • Author(s)
      川見祐介,坂口洋平,吉田秀幸,巴山忠,糸山美紀,轟木堅一郎,能田均,山口政俊
    • Journal Title

      Chromatography

      Volume: 32 (sup1) Pages: 139-140

    • Related Report
      2011 Research-status Report
  • [Presentation] フルオラス誘導体化法を利用したオキシトシンの高選択的LC-MS3分析2015

    • Author(s)
      玉嶋江莉奈,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      甲府
    • Year and Date
      2015-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] LC-MS/MSによるアミノ酸メタボロミクス -低酸素・栄養飢餓状態で培養した大腸がん細胞株DLD1について-2015

    • Author(s)
      冨田陵子,西田翔,巴山忠,轟木堅一郎,吉田秀幸,丸岡博,中島学,能田均,山口政俊,藤岡稔大
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] フルオラス誘導体化LC-MS3によるバソプレシンの高感度かつ高選択的分析法の開発2014

    • Author(s)
      竹下理紗,玉嶋江莉奈,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第32回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      北九州
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイケル付加反応を介したクルクミンのフルオラス誘導体化と選択的HPLC分析2014

    • Author(s)
      林英里奈,寺崎友理,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第32回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      北九州
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] モノリス型フルオラス固相カラムを用いたパーフルオロアルキル化アミノ酸の選択的抽出とLC-MS/MS分析2014

    • Author(s)
      玉嶋江莉奈,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第21回クロマトグラフィーシンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2014-06-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] ボロン酸のシスジオール捕獲能による蛍光誘導体化糖の選択的抽出2013

    • Author(s)
      有吉史,井上裕也,原裕太朗,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第31回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      長崎ホテル清風(長崎市)
    • Related Report
      2013 Research-status Report
  • [Presentation] エキシマー蛍光誘導体化による河川水中のビスフェノールAの液体クロマトグラフ分析2011

    • Author(s)
      山下晶平,今津佳子,吉田秀幸
    • Organizer
      第20回環境化学討論会
    • Place of Presentation
      熊本大学(熊本市)
    • Related Report
      2011 Research-status Report
  • [Presentation] エキシマー蛍光誘導体化によるジオール類のHPLC分析2011

    • Author(s)
      井上貴文,坂口洋平,佐々木知美,吉田秀幸,糸山美紀,巴山忠,能田均,山口政俊
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学(福岡市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 分散液液マイクロ抽出-誘導体化による5-ヒドロキシインドール類の高感度HPLC-蛍光分析

    • Author(s)
      岩松知美,薮内由里香,坂口洋平,巴山忠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第30回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      休暇村指宿(鹿児島県指宿市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 含水試料中アルコール類の蛍光誘導体化HPLC分析

    • Author(s)
      清川恵奈,福本麻美,糸山美紀,川見祐介,巴山忠,吉田秀幸,能田均,山口政俊
    • Organizer
      第30回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      休暇村指宿(鹿児島県指宿市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 5-ヒドロキシインドール類の高感度HPLC-蛍光分析:分散液液マイクロ抽出による誘導体化と試料濃縮

    • Author(s)
      巴山忠,岩松知美,坂口洋平,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第10回次世代を担う若手のためのフィジカル・ファーマフォーラム(PPF2012)
    • Place of Presentation
      ホテル平安の森京都(京都市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Schotten-Baunmann反応を利用した含水試料中アルコール類の蛍光誘導体化HPLC分析

    • Author(s)
      清川恵奈,福本麻美,糸山美紀,川見祐介,巴山忠,吉田秀幸,能田均,山口政俊
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学薬学部(熊本市)
    • Related Report
      2012 Research-status Report
  • [Presentation] オンラインプレカラム誘導体化によるアミノ酸のHPLC蛍光分析

    • Author(s)
      山崎見全,巴山忠,吉武誠,川見祐介,糸山美紀,吉田秀幸,能田均,山口政俊
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学薬学部(熊本市)
    • Related Report
      2012 Research-status Report
  • [Presentation] リン酸基の特異的親和性を利用した分離指向性誘導体化-HPLC分析法の開発

    • Author(s)
      川見祐介,楢崎弘毅,坂口洋平,糸山美紀,巴山忠,吉田秀幸,能田均,山口政俊
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学薬学部(熊本市)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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