Project/Area Number |
23615009
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Reliable environmental measurement methods
|
Research Institution | Fukuoka University |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
TODOROKI Kenichiro 静岡県立大学, 薬学部, 准教授 (70341451)
|
Research Collaborator |
HAYAMA Tadashi 福岡大学, 薬学部, 助教 (90549693)
TAMASHIMA Erina 福岡大学, 大学院薬学研究科
KIYOKAWA Ena 福岡大学, 大学院薬学研究科
|
Project Period (FY) |
2011-04-28 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | レドックス / 分析化学 / α-リポ酸 / エキシマー蛍光 / 蛍光誘導体化法 / クロマトグラフィー / 酸化ストレス / 蛍光誘導体化 / α-リポ酸 / リポ酸 |
Outline of Final Research Achievements |
In this research, a simultaneous determination of reduced and oxidized α-lipoic acid (LA) has been developed and was applied to the estimation of biological Red/Ox balance. This analysis method involves reversed-phase liquid chromatographic separation of LA-related compounds, on-line reduction of intramolecular disulfide structure to dithiol structure, post-column derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Balance of biological Red/Ox state were calculated from the amount of reduced-LA and that of oxidized-LA in samples. Simple, sensitive, and selective estimation of Red/Ox balance could be achieved.
|