Genome wide screening of genomic DNA demethylation factors by genetic method.
Project/Area Number |
23616002
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
epigenetics
|
Research Institution | Hiroshima University |
Principal Investigator |
KIKUCHI Yutaka 広島大学, 理学(系)研究科(研究院), 教授 (20286438)
|
Co-Investigator(Kenkyū-buntansha) |
HOZUMI Shunya 広島大学, 大学院理学研究科, 助教 (10597222)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ゼブラフィッシュ / 変異体 / スクリーニング / 脱メチル化 / 発生 / 再生 / 脱メチル化因子 |
Outline of Final Research Achievements |
Epigenetics is a regulatory system for gene expression independent of genomic DNA sequence. Genomic DNA methylation is one of epigenetics system, but the mechanisms of DNA demethylation remain to be elucidated in animal cells. To uncover the DNA demethylation systems in animal cells, I tried to screen new zebrafish mutants that have defects in DNA demethylation, but my research goal was not achieved. In addition to mutant screening, I found that both activation-induced cytidine deaminase and methyl CpG-binding domain protein 4 do not function in DNA demethylation, and the level of DNA methylation is dynamically changed during development and caudal fin regeneration in zebrafish.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Decrease in cytosine methylation at CpG island shores and increase in DNA fragmentation during zebrafish aging.2014
Author(s)
Shimoda, N., Izawa, T., Yoshizawa, A., Yokoi, H., Kikuchi, Y. and Hashimoto, N.
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Journal Title
Age
Volume: 36
Pages: 103-115
Related Report
Peer Reviewed
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[Journal Article] Expression patterns of dnmt3aa, dnmt3ab, and dnmt4 during development and fin regeneration in zebrafish.2014
Author(s)
Takayama, K., Shimoda, N., Takanaga, S., Hozumi, S. and Kikuchi, Y.
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Journal Title
Gene Expression Patterns
Volume: 14
Pages: 105-110
Related Report
Peer Reviewed
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[Journal Article] DEAD-Box Protein Ddx46 Is Required for the Development of the Digestive Organs and Brain in Zebrafish.2012
Author(s)
Hozumi, S., Hirabayashi, R., Yoshizawa, A., Ogata, M., Ishitani, T., Tsutsumi, M., Kuroiwa, A., Itoh, M. and Kikuchi, Y.
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Journal Title
Related Report
Peer Reviewed
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