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Establishment of hematopoietic differentiation system of human iPS cells in microenvironment of ovine fetuses

Research Project

Project/Area Number 23618005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionUtsunomiya University

Principal Investigator

YOSHIKAZU NAGAO  宇都宮大学, 農学部, 教授 (70291953)

Co-Investigator(Kenkyū-buntansha) FUKUMORI Rika  宇都宮大学, 農学部, 助教 (60721694)
Co-Investigator(Renkei-kenkyūsha) HANAZONO Yutaka  自治医科大学, 医学部, 教授 (70251264)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsiPS細胞 / 分化誘導 / 造血幹細胞 / 大動物 / 胎子 / 再生医学・医療 / 幹細胞 / 再生医学・医療 / ヒツジ
Outline of Final Research Achievements

We found that hematopoietic engraftment was only observed when ES cell-derivatives at the early differentiation stage were transplanted into fetal livers at 51-67 days of gestation. However, teratoma formation with mature monkey tissue structures was observed following transplantation of undifferentiated ES cells into the fetal subcutaneous tissue, but not into the liver, and at 43-50 days of gestation, but not at 51-67 days. Stem cell factor and other many factors man be concerned in the hematopoietic differentiation. Pre-conditioning by maternal administration of busulfan before in utero transplantation of monkey ES cells enhanced hematopoietic engraftments in sheep. And then, human iPS cells were transplanted into ovine fatal liver to deifferentiate into hematopoietic tissue.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (13 results)

  • [Journal Article] A long-term follow-up study on the engraftment of human hematopoietic stem cells in sheep.2014

    • Author(s)
      Tomoyuki Abe, Yutaka Hanazono, Yoshikazu Nagao
    • Journal Title

      Experimental Animals

      Volume: 63 Issue: 99999 Pages: 475-481

    • DOI

      10.1538/expanim.63.475

    • NAID

      130004701642

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Developmental Potential of Oocytes is Impaired in Cattle with Liver Abnormalities2013

    • Author(s)
      Borjigin Sarentonglaga, Kazuko Ogata, Yoriko, Taguchi, Yoku Kato, Yoshikazu Nagao
    • Journal Title

      Journal of Reproduction and Development

      Volume: 59 Pages: 168-173

    • NAID

      10031170775

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Maternal administration of busulfan before in utero transplantation of human hematopoietic stem cells enhances engraftments in sheep.2012

    • Author(s)
      Abe T, Masuda S, Tanaka Y, Nitta S, Kitano Y, Hayashi S, Hanazono Y, Nagao Y.
    • Journal Title

      Experimental Hematology

      Volume: 40 Pages: 436-444

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒツジ子宮内移植系におけるヒト造血細胞の生着・増幅技術の開発.2014

    • Author(s)
      阿部朋行、長尾慶和、柳瀬公秀、原明日香、ボラジギン・サラントラガ、緒方和子、山口美緒、花園豊.
    • Organizer
      第61回日本実験動物学会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-05-15 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of mitochondrial activity of injected sperm on early development in bovine intracytoplasmic sperm injection-derived embryos.2014

    • Author(s)
      Yoshikazu Nagao, Haruka Yamamoto, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Yoku Kato.
    • Organizer
      he 40th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Reno, Nevada, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Factors influencing engraftment of monkey embryonic stem cells in sheep after xenogeneic in utero transplantation.2013

    • Author(s)
      Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Satoshi Hayashi, Yoshihiro Kitano and Yutaka Hanazono.
    • Organizer
      The 12th Congress of the International Xenotransplantation Association.
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Long-term comparative study on the engraftment of human hematopoietic stem cells in sheep after xenogeneic in utero transplantation.2013

    • Author(s)
      Tomoyuki Abe, Shigeo Masuda, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono.
    • Organizer
      The 12th Congress of the International Xenotransplantation Association.
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] 子宮内移植後のヒツジ体内におけるヒトおよびサル造血の長期生着に及ぼす要因2012

    • Author(s)
      新田卓、阿部朋行、増田茂夫、林聡、花園豊、長尾慶和
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      つくば市
    • Related Report
      2012 Research-status Report
  • [Presentation] Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetuses2012

    • Author(s)
      Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Suguru Nitta, Borjigin Sarentonglaga, Satoshi Hayashi, Yoshihiro Kitano, Yutaka Hanazono
    • Organizer
      The 17th International Congress on Animal Reproduction
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Research-status Report
  • [Presentation] Conditioning With Busulfan Confers Engraftment Potential on Human Hematopoietic Stem Cell Comparable to HoxB4 Transduction in Sheep In Utero Transplantation2011

    • Author(s)
      Tomoyuki Abe, Shigeo Masuda, Nitta Suguru, Satoshi Hayashi, Yujiro Tanaka, Hiroshi Ban, Makoto Inoue, Mamoru Hasegawa, Yoshikazu Nagao, Yutaka Hanazono
    • Organizer
      The 9th Annual Meeting of International Society for Stem Cells Research
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒツジ胎子へのブスルファン投与はヒト造血幹細胞移植後の生着率を向上させる2011

    • Author(s)
      阿部朋行、増田茂夫、新田卓、林聡、花園豊、長尾慶和
    • Organizer
      第58回日本実験動物学会総会
    • Place of Presentation
      十和田市
    • Related Report
      2012 Research-status Report
  • [Presentation] 子宮内移植後のヒツジ体内におけるヒトおよびサル造血の長期生着に及ぼす要因2011

    • Author(s)
      新田卓、阿部朋行、増田茂夫、林聡、花園豊、長尾慶和
    • Organizer
      第104回日本繁殖生物学会大会
    • Place of Presentation
      盛岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒツジ子宮内異種移植(I):生着条件の検討

    • Author(s)
      長尾慶和、阿部朋行、柳瀬公秀、サラントラガ、緒方和子、山口美緒、林聡、花園豊
    • Organizer
      第16回日本異種移植研究会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒツジ子宮内異種移植(II):長期間の造血再構築

    • Author(s)
      阿部朋行、長尾慶和、柳瀬公秀、サラントラガ、緒方和子、山口美緒、林聡、花園豊
    • Organizer
      第16回日本異種移植研究会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒツジ胎子へのブスルファン前処置によるヒト造血幹細胞移植後の造血キメラ率の向上

    • Author(s)
      阿部朋行、増田茂夫、新田卓、林聡、長尾慶和、花園豊
    • Organizer
      第14回 日本異種移植研究会
    • Place of Presentation
      広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] 子宮内移植後のヒツジ体内におけるヒトおよびサル造血の長期生着に及ぼす要因

    • Author(s)
      新田卓、阿部朋行、増田茂夫、林聡、花園豊、長尾慶和
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      岩手県盛岡市
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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