Establishment of hematopoietic differentiation system of human iPS cells in microenvironment of ovine fetuses
| Project/Area Number |
23618005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMORI Rika 宇都宮大学, 農学部, 助教 (60721694)
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| Co-Investigator(Renkei-kenkyūsha) |
HANAZONO Yutaka 自治医科大学, 医学部, 教授 (70251264)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | iPS細胞 / 分化誘導 / 造血幹細胞 / 大動物 / 胎子 / 再生医学･医療 / 幹細胞 / 再生医学・医療 / ヒツジ |
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| Outline of Final Research Achievements |
We found that hematopoietic engraftment was only observed when ES cell-derivatives at the early differentiation stage were transplanted into fetal livers at 51-67 days of gestation. However, teratoma formation with mature monkey tissue structures was observed following transplantation of undifferentiated ES cells into the fetal subcutaneous tissue, but not into the liver, and at 43-50 days of gestation, but not at 51-67 days. Stem cell factor and other many factors man be concerned in the hematopoietic differentiation. Pre-conditioning by maternal administration of busulfan before in utero transplantation of monkey ES cells enhanced hematopoietic engraftments in sheep. And then, human iPS cells were transplanted into ovine fatal liver to deifferentiate into hematopoietic tissue.
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Report
(5 results)
Research Products
(16 results)
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[Presentation] Factors influencing engraftment of monkey embryonic stem cells in sheep after xenogeneic in utero transplantation.2013
Author(s)
Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Satoshi Hayashi, Yoshihiro Kitano and Yutaka Hanazono.
Organizer
The 12th Congress of the International Xenotransplantation Association.
Place of Presentation
Osaka, Japan
Related Report
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[Presentation] Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetuses2012
Author(s)
Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Suguru Nitta, Borjigin Sarentonglaga, Satoshi Hayashi, Yoshihiro Kitano, Yutaka Hanazono
Organizer
The 17th International Congress on Animal Reproduction
Place of Presentation
Vancouver, Canada
Related Report
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[Presentation] Conditioning With Busulfan Confers Engraftment Potential on Human Hematopoietic Stem Cell Comparable to HoxB4 Transduction in Sheep In Utero Transplantation2011
Author(s)
Tomoyuki Abe, Shigeo Masuda, Nitta Suguru, Satoshi Hayashi, Yujiro Tanaka, Hiroshi Ban, Makoto Inoue, Mamoru Hasegawa, Yoshikazu Nagao, Yutaka Hanazono
Organizer
The 9th Annual Meeting of International Society for Stem Cells Research
Place of Presentation
Toronto, Canada
Related Report
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