• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Safety and effectiveness of neural stem cells directly induced from skin fibroblasts

Research Project

Project/Area Number 23618010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionKeio University

Principal Investigator

AKAMATSU WADO  慶應義塾大学, 医学部, 講師 (60338184)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords神経幹細胞 / ダイレクトリプログラミング / リプログラミング / 直接誘導 / iPS細胞
Research Abstract

We succeeded in making human directly induced neural stem cells by using Sendai virus vectors. Although we were able to obtain increased number of neurospheres compared to retroviral vectors, SeV-induced neurospheres did not differentiate into neural cells efficiently.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (55 results)

All 2014 2013 2012 2011 2009 Other

All Journal Article (23 results) (of which Peer Reviewed: 21 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (31 results) (of which Invited: 29 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Involvement of ER Stress in Dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 Missense Mutations Shown by iPSC-Derived Oligodendrocytes2014

    • Author(s)
      Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Oasaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    • Journal Title

      Stem Cell Rep

      Volume: 2 Issue: 5 Pages: 1-14

    • DOI

      10.1016/j.stemcr.2014.03.007

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors.2014

    • Author(s)
      Zhou Z, Kohda K, Ibata K, Kohyama J, Akamatsu W, Yuzaki M, Okano HJ, Sasaki E, Okano H
    • Journal Title

      Molecular Brain

      Volume: 3 Issue: 1 Pages: 724-724

    • DOI

      10.1186/1756-6606-7-24

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Prenatal deletion of the RNA binding protein HuD disrupts postnatal cortical circuit maturation and behavior2014

    • Author(s)
      DeBoer E, Azevedo R, Vega T, Brodkin J, Akamatsu W, Okano H, Wagner G, Rasin MR
    • Journal Title

      J Neurosci

      Volume: 34(10) Pages: 3674-86

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The RNA-binding protein HuD regulates autophagosome formation in pancreatic β cells by promoting autophagy-related gene 5 expression.2014

    • Author(s)
      Kim C, Kim W, Lee H, Ji E, Choe YJ, Martindale JL, Akamatsu W, Okano H, Kim HS, Nam SW, Gorospe M, Lee EK.
    • Journal Title

      J Biol Chem.

      Volume: 289 Issue: 1 Pages: 112-121

    • DOI

      10.1074/jbc.m113.474700

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased l1 retrotransposition in the neuroral genome in schizophrenia.2014

    • Author(s)
      Miki Bundo, Manabu Toyoshima, Yohei Okada, Wado Akamatsu, Junko Ueda, Taeko Nemoto-Miyauchi, Fumiko Sunaga, Michihiro Toritsuka, Daisuke Ikawa, Akiyoshi Kakita, Motoichiro Kato, Kiyoto Kasai, Toshifumi Kishimoto, Hiroyuki Nawa, Hideyuki Okano, Takeo Yoshikawa, Tadafumi Katoemail, Kazuya Iwamoto
    • Journal Title

      Neuron

      Volume: 81 Issue: 2 Pages: 306-313

    • DOI

      10.1016/j.neuron.2013.10.053

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prenatal Deletion of the RNA-Binding Protein HuD Disrupts Postnatal Cortical Circuit Maturation and Behavior.2014

    • Author(s)
      Deboer EM, Azevedo R, Vega TA, Brodkin J, Akamatsu W, Okano H, Wagner GC, Rasin MR.
    • Journal Title

      J Neurosci.

      Volume: 34 Issue: 10 Pages: 3674-3686

    • DOI

      10.1523/jneurosci.3703-13.2014

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A human Dravet syndrome model from patient induced pluripotent stem cells.2013

    • Author(s)
      Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori MX, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S.
    • Journal Title

      Mol Brain

      Volume: 6 Issue: 1 Pages: 19-19

    • DOI

      10.1186/1756-6606-6-19

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of human melanocytes from induced pluripotent stem cells.2013

    • Author(s)
      Ohta S, Imaizumi Y, Akamatsu W, Okano H, Kawakami Y.
    • Journal Title

      Methods Mol Biol.

      Volume: 989 Pages: 193-215

    • DOI

      10.1007/978-1-62703-330-5_16

    • ISBN
      9781627033299, 9781627033305
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Enhanced aggregation of androgen receptor in induced pluripotent stem cell-derived neurons from spinal and bulbar muscular atrophy.2013

    • Author(s)
      Nihei Y, Ito D, Okada Y, Akamatsu W, Yagi T, Yoshizaki T, Okano H, Suzuki N.
    • Journal Title

      J Biol Chem.

      Volume: 288 Issue: 12 Pages: 8043-8052

    • DOI

      10.1074/jbc.m112.408211

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo2013

    • Author(s)
      Veraitch O, Kobayashi T, Imaizumi Y, Akamatsu W, Sasaki T, Yamanaka S, Amagai M, Okano H and Ohyama M
    • Journal Title

      J Invest Dermatol

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo.2013

    • Author(s)
      Veraitch O, Kobayashi T, Imaizumi Y, Akamatsu W, Sasaki T, Yamanaka S, Amagai M, Okano H and Ohyama M
    • Journal Title

      J Invest Dermatol.

      Volume: - Issue: 6 Pages: 1479-1488

    • DOI

      10.1038/jid.2013.7

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 疾患特異的iPS細胞研究の基礎と実際2013

    • Author(s)
      赤松 和土
    • Journal Title

      バイオクリニカ

      Volume: 28 Pages: 22-26

    • Related Report
      2012 Research-status Report
  • [Journal Article] 神経系のiPS technologyの今後の展望2013

    • Author(s)
      赤松 和土
    • Journal Title

      モダンフィジシャン

      Volume: 33 Pages: 696-698

    • Related Report
      2012 Research-status Report
  • [Journal Article] Mitochondrial dysfunction associated with increased oxidative stress and -synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue.2012

    • Author(s)
      Imaizumi Y
    • Journal Title

      Mol Brain

      Volume: 5 Issue: 1 Pages: 35-35

    • DOI

      10.1186/1756-6606-5-35

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration of the damaged central nervous system through reprog ramming technology : Basic concepts and potential application for cell replacement therapy2012

    • Author(s)
      Matsui T, Akamatsu W, Nakamura M, Okano H
    • Journal Title

      Exp Neurol

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Derivation of induced pluripotent stem cells by retroviral gene transduction in Mammalian species2012

    • Author(s)
      Imamura, M.
    • Journal Title

      Methods Mol Biol

      Volume: 925 Pages: 21-48

    • DOI

      10.1007/978-1-62703-011-3_2

    • ISBN
      9781627030106, 9781627030113
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research2012

    • Author(s)
      Yagi, T.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 7 Pages: e41572-e41572

    • DOI

      10.1371/journal.pone.0041572

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neural Stem Cells Directly Differentiated from Partially Reprogrammed Fibroblasts Rapidly Acquire Gliogenic Competency2012

    • Author(s)
      Matsui T, Takano M, Yoshida K, Ono S, Fujisaki C, Matsuzaki Y, Toyama Y, Nakamura M, Okano H, Akamatsu W
    • Journal Title

      Stem cells

      Volume: (In press) Issue: 6 Pages: 1109-1119

    • DOI

      10.1002/stem.1091

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA-binding protein HuD controls insulin translation2012

    • Author(s)
      Lee EK, Kim W, Tominaga K, Martindale JL, Yang X, Subaran SS, Carlson OD, Mercken EM, Kulkarni RN, Akamatsu W, Okano H, Perrone-Bizzozero NI, de Cabo R, Egan JM, Gorospe M
    • Journal Title

      Mol Cell

      Volume: 45 (6) Issue: 6 Pages: 826-35

    • DOI

      10.1016/j.molcel.2012.01.016

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration of the damaged central nervous system through reprogramming technology: Basic concepts and potential application for cell replacement therapy.2012

    • Author(s)
      Matsui T, Akamatsu W, Nakamura M, Okano H.
    • Journal Title

      Exp Neurol.

      Volume: 00 Pages: 0-0

    • DOI

      10.1016/j.expneurol.2012.09.016

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA-Binding Protein HuD Controls Insulin Translation2012

    • Author(s)
      Lee EK, Kim W, Tominaga K, Martindale JL, Yang X, Subaran SS, Carlson OD, Mercken EM, Kulkarni RN, Akamatsu W, Okano H, Perrone-Bizzozero NI, de Cabo R, Egan JM, Gorospe M.
    • Journal Title

      Molecular Cell

      Volume: 45(6) Pages: 826-35

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modeling familial Alzheimer's disease with induced pluripotentstem cells2011

    • Author(s)
      Yagi T, Ito D, Okada Y, Akamatsu W, Nihei Y, Yoshizaki T, Yamanaka S, Ok ano H, Suzuki N
    • Journal Title

      Hum Mol Genet

      Volume: 20(23) Pages: 4530-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modeling familial Alzheimer's disease with induced pluripotent stem cells.2011

    • Author(s)
      Yagi T, Ito D, Okada Y, Akamatsu W, Nihei Y, Yoshizaki T, Yamanaka S, Okano H, Suzuki N
    • Journal Title

      Human Molecular Genetics

      Volume: 20(23) Pages: 4530-9

    • NAID

      130004505083

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 多能性幹細胞(ES細胞/iPS細胞)を用いた神経疾患の再生医療と病態研究2014

    • Author(s)
      赤松和土
    • Organizer
      順天堂大学第136回お茶の水木曜会
    • Place of Presentation
      東京
    • Year and Date
      2014-03-13
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Direct Reprogrammingの最近の進歩:神経幹細胞の直接誘導とその応用2014

    • Author(s)
      赤松和土
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都(シンポジウム)
    • Year and Date
      2014-03-05
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞技術を用いた神経疾患研究と治療2013

    • Author(s)
      赤松和土
    • Organizer
      第37回日本血液事業学会総会
    • Place of Presentation
      札幌(シンポジウム)
    • Year and Date
      2013-10-21
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS technology-based regenerative medicine for damaged central nervous system2013

    • Author(s)
      赤松和土
    • Organizer
      第11回遺伝子治療学会
    • Place of Presentation
      岡山(シンポジウム)
    • Year and Date
      2013-07-06
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 疾患iPS細胞と創薬:「神経疾患患者由来細胞からの神経系細胞の誘導と病態解析」2013

    • Author(s)
      赤松和土
    • Organizer
      炎症再生学会
    • Place of Presentation
      京都(シンポジウム)
    • Year and Date
      2013-07-02
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞を使った神経疾患の治療法の開発2013

    • Author(s)
      赤松和土
    • Organizer
      第20回東京血管疾患研究所セミナー
    • Place of Presentation
      東京
    • Year and Date
      2013-06-08
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞技術を用いた神経系の再生医療と疾患解析2013

    • Author(s)
      赤松和土
    • Organizer
      第2回小児泌尿器科研究会
    • Place of Presentation
      東京
    • Year and Date
      2013-06-01
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 多能性幹細胞を用いた神経疾患の再生医療と病態研究2013

    • Author(s)
      赤松和土
    • Organizer
      第11回横浜小児先端医療セミナー
    • Place of Presentation
      横浜
    • Year and Date
      2013-05-24
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞技術の神経疾患研究・治療への応用2013

    • Author(s)
      赤松和土
    • Organizer
      第116回日本小児科学会学術集会
    • Place of Presentation
      広島
    • Year and Date
      2013-04-19
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 45 iPS細胞技術を用いた中枢神経系の再生と創薬研究:iPS technology-based cell therapy for damaged CNS and investigation of neural disorders2013

    • Author(s)
      赤松和土,岡野栄之
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京(シンポジウム)
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 疾患特異的iPS細胞を用いた病態解明および治療法確立研究:神経疾患患者由来細胞からの神経系細胞の誘導と病態解析2013

    • Author(s)
      赤松和土
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      東京(シンポジウム)
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 多能性幹細胞・神経幹細胞を用いた再生医療と病態解析2013

    • Author(s)
      赤松和土
    • Organizer
      愛知医科大学細胞治療研究会
    • Place of Presentation
      愛知
    • Year and Date
      2013-03-05
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ALSに対する再生医療の開発:幹細胞生物学を応用した神経疾患病態研究2012

    • Author(s)
      赤松和土
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2012-05-23
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 多能性幹細胞から神経幹細胞を生み出す分子機構とその応用2012

    • Author(s)
      赤松和土
    • Organizer
      第12回日本分子生物学会春季シンポジウム
    • Place of Presentation
      山梨
    • Year and Date
      2012-04-26
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 13基礎研究セミナー:多能性幹細胞由来神経幹細胞を用いた神経系の再生医療の展望2012

    • Author(s)
      赤松和土
    • Organizer
      第116回日本眼科学会
    • Place of Presentation
      東京(シンポジウム)
    • Year and Date
      2012-04-06
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] "ヒト"をキーワードにした多能性幹細胞研究の展開:体細胞からの安全な神経幹細胞の直接誘導2012

    • Author(s)
      赤松和土
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      長野(シンポジストおよび座長)
    • Year and Date
      2012-03-31
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 体細胞からの安全な神経幹細胞の直接誘導2012

    • Author(s)
      赤松和土
    • Organizer
      第89回日本生理学会大会・企画シンポジウム(招待講演)
    • Place of Presentation
      松本文化会館・長野
    • Related Report
      2011 Research-status Report
  • [Presentation] 多能性幹細胞を用いた神経系の再生医療の展望2011

    • Author(s)
      赤松和土,岡野栄之
    • Organizer
      長野県医学会例会
    • Place of Presentation
      長野(特別講演)
    • Year and Date
      2011-07-25
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞技術の神経疾患研究・治療への応用

    • Author(s)
      赤松 和土, 岡野 栄之
    • Organizer
      第116回日本小児科学会学術集会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 多能性幹細胞を用いた神経疾患の再生医療と病態研究

    • Author(s)
      赤松 和土
    • Organizer
      第11回横浜小児先端医療セミナー
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS細胞技術を用いた神経系の再生医療と疾患解析

    • Author(s)
      赤松 和土
    • Organizer
      第2回小児泌尿器科研究会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS細胞を使った神経疾患の治療法の開発

    • Author(s)
      赤松 和土
    • Organizer
      第20回東京血管疾患研究所セミナー
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS technology-based regenerative medicine for damaged central nervous system

    • Author(s)
      赤松 和土
    • Organizer
      第11回遺伝子治療学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS 細胞技術を用いた神経疾患研究と治療

    • Author(s)
      赤松 和土
    • Organizer
      第37 回日本血液事業学会総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Direct Reprogrammingの最近の進歩:神経幹細胞の直接誘導とその応用

    • Author(s)
      赤松 和土
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 多能性幹細胞(ES細胞/iPS細胞)を用いた神経疾患の再生医療と病態研究

    • Author(s)
      赤松 和土
    • Organizer
      順天堂大学第136回お茶の水木曜会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 多能性幹細胞由来神経幹細胞を用いた神経系の再生医療の展望

    • Author(s)
      赤松 和土
    • Organizer
      第116回日本眼科学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 多能性幹細胞から神経幹細胞を生み出す分子機構とその応用

    • Author(s)
      赤松 和土
    • Organizer
      第12回日本分子生物学会春季シンポジウム
    • Place of Presentation
      山梨
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 幹細胞生物学を応用した神経疾患病態研究

    • Author(s)
      赤松 和土
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 神経疾患患者由来細胞からの神経系細胞の誘導と病態解析

    • Author(s)
      赤松 和土
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] PS細胞技術を用いた中枢神経系の再生と創薬研究

    • Author(s)
      赤松 和土, 岡野 栄之
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 神経幹細胞製造方法2009

    • Inventor(s)
      岡野栄之、赤松和土
    • Industrial Property Rights Holder
      岡野栄之、赤松和土
    • Industrial Property Rights Type
      特許
    • Filing Date
      2009-11-04
    • Related Report
      2013 Final Research Report
    • Overseas

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi