Mechanism underlying photoreceptor degeneration in response to vesicular fusion defects

• Project/Area Number: 23650173
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: 沖縄科学技術大学院大学
• Principal Investigator: MASAI Ichiro 沖縄科学技術大学院大学, 神経発生ユニット, 准教授 (50242087)
• Co-Investigator(Renkei-kenkyūsha): NISHIWAKI Yuko 沖縄科学技術大学院, 神経発生ユニット, 研究員 (20360620)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経科学 / 脳神経疾患 / 視細胞変性 / 遺伝疾患 / アポトーシス / 小胞輸送 / ゼブラフィッシュ / BH3-only蛋白質 / 細胞死 / β-SNAP / BNip1 / BH3蛋白質

Research Abstract

There are hereditary photoreceptor degeneration diseases in human. While previous studies suggested a connection between defects in vesicular transport and photoreceptor degeneration, the mechanism behind this link remained elusive. In this study, we investigated the mechanism underlying photoreceptor degeneration using zebrafish and identified BNip1 as a factor that induces cell death in response to vesicular transport defects. This finding uncovers a novel mechanism of photoreceptor degeneration and may advance our understanding of pathological process of human genetic retinal disease.

Research Products

• [Journal Article] The BH3-only SNARE BNip1 mediates photoreceptor apoptosis in response to vesicular fusion defects (2013)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Journal Title: Developmental Cell Volume: 25 Issue: 4 Pages: 374-387
  • DOI: 10.1016/j.devcel.2013.04.015
  • Peer Reviewed
• [Journal Article] (*equal contribution; #corresponding authors) Ectopic expression of the cone-specific Gprotein-coupled receptor kinase GRK7 in zebrafish rods leads to decreased photosensitivity and compromised visual performance (2011)
  • Author(s): Vogalis,F., Shiraki,T., Kojima,D., Fukada,Y. & Lamb,T.
  • Journal Title: J. Physiol. (London) Volume: 589 Issue: 9 Pages: 2321-2348
  • DOI: 10.1113/jphysiol.2010.204156
  • Peer Reviewed
• [Presentation] Syntaxin18cis-SNARE complex is a novel alarm factorthat detects vesicular transport defects (2013)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: The 46th Annual Meeting of the Japanese Society of Developmental Biologists.
  • Place of Presentation: Matsue, Shimane Japan
• [Presentation] Characterization of a zebrafish mutantshowing cone photoreceptor degeneration (2013)
  • Author(s): Iribarne, M., Nishiwaki, Y., and Masai, I.
  • Organizer: The 46th Annual Meeting of the Japanese Society of Developmental Biologists Matsue,
  • Place of Presentation: Shimane, Japan
• [Presentation] Syntaxin18 cis-SNARE complex is a novel alarm factor that detects vesicular transport defects (2013)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: The 46th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: Matsue, Japan
• [Presentation] Characterization of a zebrafish mutant showing cone photoreceptor degeneration (2013)
  • Author(s): Iribarne, M., Nishiwaki, Y., and Masai, I.
  • Organizer: The 46th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: Matsue, Japan
• [Presentation] Geneticmutation of beta-SNAP causes photoreceptordegeneration through the activation of theBH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: 10th International Conference Zebrafish Development and Genetics
  • Place of Presentation: Madison, USA
• [Presentation] Genetic mutation of beta-SNAP causes photoreceptor degeneration through the activation of the BH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: APDBC Taiwan 2012 Asia-Pacific Developmental Biology Conference 2012
  • Place of Presentation: Taipei Innovation City Convention Center, Taipei, Taiwan
• [Presentation] The BH3-only SNARE of syntaxin18, BNip1, mediates photoreceptor apoptosis in response to vesicular fusion defects (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: Gordon researchconference ? visual system development.
  • Place of Presentation: Colby-Sawyer College, New London, NH
• [Presentation] The BH3-onlySNARE of syntaxin18, BNip1, is a mediatorof photoreceptor apoptosis in response tovesicular fusion defects (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: The 35th annual meeting of the Molecular Biology Society
  • Place of Presentation: Japan. Fukuoka
• [Presentation] Genetic mutation of beta-SNAP causes photoreceptor degeneration through the activation of the BH3-only SNARE,BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: The 18th Japanese Medaka and zebrafish meeting.
  • Place of Presentation: Kyoto, Japan
• [Presentation] The BH3-onlySNARE of syntaxin18, BNip1, is a mediatorof photoreceptor apoptosis in response tovesicular fusion defects (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: Neuroscience 2012.The 35thannual meeting of the JapanNeuroscience Society.
  • Place of Presentation: Nagoya, Japan
• [Presentation] Geneticmutation of beta-SNAP causesphotoreceptor degeneration through theactivation of the BH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y.,Oguri, E., Nakamura, S., Suzuki, S.,Yuasa-Kawada, J., Kinoshita-Kawada, M.,Mochizuki, T., and Masai, I.
  • Organizer: Joint meeting of the 45th annual meeting of the Japanese society of DevelopmentalBiologists & the 64thannual meeting of theJapanese society of Cell Biology.
  • Place of Presentation: Kobe,Japan
• [Presentation] Genetic mutation of beta-SNAP causes photoreceptor degeneration through the activation of the BH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: 10th International Conference Zebrafish Development and Genetics
  • Place of Presentation: Madison, USA
• [Presentation] Genetic mutation of beta-SNAP causes photoreceptor degeneration through the activation of the BH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: APDBC Taiwan 2012 Asia-Pacific Developmental Biology Conference 2012
  • Place of Presentation: Taipei, Taiwan
• [Presentation] The BH3-only SNARE of syntaxin18, BNip1, mediates photoreceptor apoptosis in response to vesicular fusion defects (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: Gordon research conference visual system development
  • Place of Presentation: New London, USA
• [Presentation] The BH3-only SNARE of syntaxin18, BNip1, is a mediator of photoreceptor apoptosis in response to vesicular fusion defects (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: The 35th annual meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Genetic mutation of beta-SNAP causes photoreceptor degeneration through the activation of the BH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: The 18th Japanese Medaka and zebrafish meeting
  • Place of Presentation: Kyoto, Japan
• [Presentation] The BH3-only SNARE of syntaxin18, BNip1, is a mediator of photoreceptor apoptosis in response to vesicular fusion defects (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: The 35th annual meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya, Japan
• [Presentation] Genetic mutation of beta-SNAP causes photoreceptor degeneration through the activation of the BH3-only SNARE, BNip1 (2012)
  • Author(s): Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
  • Organizer: Joint meeting of the 45th annual meeting of the Japanese society of Developmental Biologists & the 64th annual meeting of the Japanese society of Cell Biology
  • Place of Presentation: Kobe, Japan
• [Presentation] Molecular mechanism that transforms defects in intracellular vesicular transport into apoptosis in zebrafishphotoreceptors (2011)
  • Author(s): Masai, I.
  • Organizer: 5th Asia-Oceania zebrafish international meeting, China National Convention Center
  • Place of Presentation: Beijing, China
• [Presentation] Mutation of beta-SNAP induces amitochondria-dependent apoptosis inzebrafish photoreceptors (2011)
  • Author(s): Nishiwaki, Y., Suzuki, S., Yoshizawa, A.,Kojima, Y., Mochizuki, T., Masai, I.
  • Organizer: 7th EuropeanZebrafish Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Azebrafish corona mutation induces aBcl2-dependent apoptosis in photoreceptor (2011)
  • Author(s): Nishiwaki, Y., Suzuki, S., Yoshizawa, A.,Kojima, Y., Mochizuki, T., Masai, I.
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: Okinawa Convention Center (Ginowan City)
• [Presentation] Molecular mechanism that transforms defects in intracellular vesicular transport into apoptosis in zebrafish photoreceptors (2011)
  • Author(s): 政井一郎
  • Organizer: 第5回 アジアオセアニア ゼブラフィッシュ国際会議
  • Place of Presentation: 北京、中国
• [Presentation] A zebrafish corona mutation induces a Bcl2-dependent apoptosis in photoreceptor (2011)
  • Author(s): 西脇優子、鈴木祥平、吉澤あすか、小島豊、望月俊昭、政井一郎
  • Organizer: 第44回 日本発生生物学会年会
  • Place of Presentation: 沖縄、日本
• [Presentation] Mutation of beta-SNAP induces a mitochondria-dependent apoptosis in zebrafish photoreceptors (2011)
  • Author(s): 西脇優子、鈴木祥平、吉澤あすか、小島豊、望月俊昭、政井一郎
  • Organizer: 第7回 ヨーロッパ ゼブラフィッシュ国際会議
  • Place of Presentation: エジンバラ、英国
• [Remarks] 目の見えないゼブラフィッシュがヒトの網膜疾患治療に光をもたらす
  • URL: http://www.oist.jp/ja/news-center/press-releases/10744