Visualization and molecular mechanisms underlying neural polarity protein SAD kinase-mediated phosphorylation

• Project/Area Number: 23650188
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Yamanashi
• Principal Investigator: OHTSUKA Toshihisa 山梨大学, 大学院・医学工学総合研究部, 教授 (40401806)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞内情報伝達 / シナプス / リン酸化 / アクティブゾーン

Research Abstract

SAD kinase, a serine/threonine kinase associated with synapticvesicles and active zones in the nerve terminals, has shown to phosphorylate an active zone protein specifically. An anti-phospho antibody against thephosphorylated sequence was produced and su

Research Products

• [Journal Article] functional scaffold for the integrity of the presynaptic active zone (2013)
  • Author(s): Ohtsuka, T. CAST
  • Journal Title: Neurosci. Res
  • Peer Reviewed
• [Journal Article] CAST: functional scaffold for the integrity of the presynaptic active (2013)
  • Author(s): Ohtsuka, T.
  • Journal Title: Neurosci. Res. Volume: in press Issue: 1-2 Pages: 10-15
  • DOI: 10.1016/j.neures.2013.03.003
  • Peer Reviewed
• [Journal Article] Deletion of the presynaptic scaffold CAST reduces active zone size in rod photoreceptors and impairs visual processing (2012)
  • Author(s): tom Dieck, S., Specht, D., Strenzke, N., Hida, Y., Krishnamoorthy, V., Inoue, E., Ishizaki, H., Tanaka-Okamoto, M., Miyoshi, J., Hagiwara, A., Brandstatter, JH., Gollisch, T., Ohtsuka, T. *, Moser, T*
  • Journal Title: J. Neurosci Volume: 32 Pages: 12192-12203
  • Peer Reviewed
• [Journal Article] Physical and Functional Interaction of the Active Zone Protein CAST/ERC2 and the ss-subunit of the Voltage-dependent Ca^Channel (2012)
  • Author(s): Kiyonaka, S., Nakajima, H., Takada, Y., Hida, Y., Yoshioka, T., Hagiwara, A., Kitajima, I., Mori, Y., Ohtsuka, T
  • Journal Title: J. Biochem Volume: 152 Pages: 149-159
  • Peer Reviewed
• [Journal Article] Deletion of the presynaptic scaffold CAST reduces active zone size in rod photoreceptors and impairs visual processing. (2012)
  • Author(s): tom Dieck, S.
  • Journal Title: J. Neurosci. Volume: 32 Pages: 12192-12203
  • Peer Reviewed
• [Journal Article] Physical and Functional Interaction of the Active Zone Protein CAST/ERC2 and the -subunit of the Voltage-dependent Ca2+ Channel. (2012)
  • Author(s): Kiyonaka, S.
  • Journal Title: J. Biochem. Volume: 152 Pages: 149-159
  • Peer Reviewed
• [Journal Article] Physical and Functional Interaction of the Active Zone Protein CAST/ERC2 and the beta-subunit of the Voltage-dependent Ca2+ Channel. (2012)
  • Author(s): Kiyonaka, S.
  • Journal Title: J. Biochem. Volume: in press
  • Peer Reviewed
• [Journal Article] Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse. (2011)
  • Author(s): Hagiwara, A.
  • Journal Title: NeuroReport Volume: 22 Pages: 319-325
  • Peer Reviewed
• [Journal Article] Prickle2 is localized in the postsynaptic density and interacts with PSD-95 and NMDA receptors in the brain. (2011)
  • Author(s): Hida, Y.
  • Journal Title: J. Biochem. Volume: 149 Pages: 693-700
  • Peer Reviewed
• [Presentation] Structure and function of the presynaptic active zone (2012)
  • Author(s): Toshihisa Ohtsuka
  • Organizer: 24年度国際シナプス研究会
  • Place of Presentation: 岡崎
• [Presentation] Structure and function of the presynaptic active zone (2012)
  • Author(s): Ohtsuka Toshihisa
  • Organizer: 24年度国際シナプス研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Invited
• [Presentation] 翻訳後修飾によるプレシナプス・アクティブゾーンの機能制御 (2012)
  • Author(s): 大塚稔久
  • Organizer: 生理学研究所シナプス研究会「神経シナプス伝達の時空間ダイナミクス」
  • Place of Presentation: 自然科学研究機構（愛知県岡崎市）
  • Invited
• [Presentation] Role of CAST in the structure and function of ribbon synapses (2011)
  • Author(s): Toshihisa Ohtsuka
  • Organizer: Ribbon Synapses Symposium
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2011-09-18
• [Presentation] Role of CAST in the structure and function of ribbon synapses (2011)
  • Author(s): Toshihisa Ohtsuka
  • Organizer: Ribbon Synapses Sympojium(招待講演)
  • Place of Presentation: Germany
• [Book] Brain & Nerve (2011)
  • Author(s): 大塚稔久
  • Total Pages: 8
  • Publisher: 医学書院
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/bioche01/
• [Remarks] 生化学講座第１教室ＨＰ
  • URL: http://www.med.yamanashi.ac.jp/basic/bioche01/