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Local translation of nuclear proteins at the synapses, translocation to the nucleus and synaptic plasticity.

Research Project

Project/Area Number 23650189
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionShinshu University

Principal Investigator

SHIRAI Yoshinori  信州大学, 医学系研究科, 助教 (70342798)

Co-Investigator(Kenkyū-buntansha) SUZUKI Tatsuo  信州大学, 医学系研究科, 教授 (80162965)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsmRNA / 局所翻訳 / 核移行 / 遺伝子発現 / シナプス可塑性 / 核タンパク質 / 非翻訳領域
Research Abstract

We investigated promoter region of rat Gtf2i gene and predicted alternative transcription start points of Gtf2i mRNA based on cap analysis gene expression (CAGE) database. Using these information, we identified seven novel transcripts of Gtf2i with different 5'UTRs in rat brain. We examined expression of these novel Gtf2i mRNAs with alternative 5'UTRs in rat brain by in situ hybridization. Gtf2i mRNAs with different 5'UTRs showed differential expression patterns and some of them were detected in dendritic processed of neuronal cells, suggestion that Gtf2i mRNA with these 5'UTRs exist in neuronal dendrite/PSD. We identified four novel splicing variants of the coding sequence of Gtf2i in addition to two known variants in rat brain.
These results suggest 5'UTRs of Gtf2i mRNA play important roles in expression of Gtf2i gene in the brain: differential cellular expression via alternative promoter usage, mRNA localization to and spatiotemporally specific translation in neuronal dendrites.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (6 results)

All 2013 Other

All Presentation (2 results) Remarks (4 results)

  • [Presentation] CAGE データベースを用いた Gtf2i の新規な 5' UTR の同定および脳での発現2013

    • Author(s)
      白井良憲・鈴木龍雄
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] CAGEデータベースを用いたGtf2iの新規な5'UTRsの同定および脳での発現

    • Author(s)
      白井良憲、鈴木龍雄
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.shinshu-u.ac.jp/faculty/medicine/department/doctor/grdkarei/ikaso

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfeuNkh.html

    • Related Report
      2013 Final Research Report
  • [Remarks] SOAR研究者総覧 信州大学学術情報オンラインシステムSOAR

    • URL

      http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfeuNkh.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 信州大学大学院 医学系研究科 神経可塑性学教室ホームページ

    • URL

      http://www.shinshu-u.ac.jp/faculty/medicine/department/doctor/grdkarei/i-kaso/

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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