Animal models for hydatid development of new species Echinococcus shiquicus

• Project/Area Number: 23650226
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Asahikawa Medical College
• Principal Investigator: NAKAYA Kazuhiro 旭川医科大学, 医学部, 准教授 (70109388)
• Co-Investigator(Kenkyū-buntansha): ITO Akira 旭川医科大学, 医学部, 客員教授 (70054020)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Echinococcus shiquicus / チベット包虫 / scid マウス / 嚢包 / 増殖形態 / 凍結保存 / in vitro

Research Abstract

This study is carried out about the hydatid metacestode of new species Echinococcus shiquicus which was discovered in the Qinghai-Tibet plateau region of China in 2005. We used two strains of experimental mice, NOD/Shi-scid and BALB/cA, for the development and multiplication of metacestode. The parasite freezing preservation with Liquid nitrogen and the culture of vesicle were succeeded. From now on, these results will bring of more effects for the future studies of this parasite.

Research Products

• [Journal Article] Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): Proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria (2013)
  • Author(s): Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y, Okamoto M, Ito A.
  • Journal Title: Int J Parasitol Volume: 43(6) Pages: 427-37
• [Journal Article] Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): Proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria (2013)
  • Author(s): Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y, Okamoto M, Ito A.
  • Journal Title: International Journal for Parasitology Volume: 43(6) Issue: 6 Pages: 427-37
  • DOI: 10.1016/j.ijpara.2012.11.014
  • Peer Reviewed
• [Journal Article] An ocular cysticercosis in Bali, Indonesia caused by Taenia solium Asian genotype (2012)
  • Author(s): Swastika K, Dewiyani CI, Yanagida T, Sako Y, Sudarmaja M, Sutisna P, Wandra T, Dharmawan NS, Nakaya K, Okamoto M, Ito A.
  • Journal Title: Parasitol Int Volume: 61(2) Pages: 378-80
• [Journal Article] A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey (2012)
  • Author(s): Nkouawa A, Sako Y, Li T, Chen X, Nakao M, Yanagida T, Okamoto M, Giraudoux P, Raoul F, Nakaya K, Xiao N, Qiu J, Qiu D, Craig PS, Ito A.
  • Journal Title: Parasitol Int Volume: 61(4) Pages: 723-5
• [Journal Article] Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East (2012)
  • Author(s): Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi SM, Hijjawi N, Abdel-Hafez SK, Sako Y, Okamoto M, Ito A.
  • Journal Title: Parasitol Int Volume: 61(4) Pages: 599-603
• [Journal Article] Taeniasis and cysticercosis due to Taenia solium in Japan (2012)
  • Author(s): Yanagida T, Sako Y, Nakao M, Nakaya K, Ito A.
  • Journal Title: Parasit Vectors Volume: 17(5) Pages: 18-18
  • NAID: 120006522746
• [Journal Article] An ocular cysticercosis in Bali, Indonesia caused by Taenia solium Asian genotype (2012)
  • Author(s): Swastika K, et al.
  • Journal Title: Parasitology International Volume: 61 Issue: 2 Pages: 378-380
  • DOI: 10.1016/j.parint.2011.11.004
  • Peer Reviewed
• [Journal Article] A Loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey (2012)
  • Author(s): Nkouawa A, Sako Y, Li T, Chen X, Nakao M, Yanagida T, Okamoto M, Giraudoux P, Raoul F, Nakaya K, Xiao N, Qiu J, Qiu D, Craig PS, Ito A.
  • Journal Title: Parasitol Int Volume: 61 Issue: 4 Pages: 723-725
  • DOI: 10.1016/j.parint.2012.06.001
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms of Echinococcus granulosus in the Middle East (2012)
  • Author(s): Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi SM, Hijjawi N, Abdel-Hafez SK, Sako Y, Okamoto M, Ito A
  • Journal Title: Parasitol Int Volume: 61 Issue: 4 Pages: 599-603
  • DOI: 10.1016/j.parint.2012.05.014
  • Peer Reviewed
• [Journal Article] Taeniasis and cysticercosis due to Taenia solium in Japan (2012)
  • Author(s): Yanagida T, et al.
  • Journal Title: Parasites and Vectors Volume: 5:18 Issue: 1 Pages: 1-6
  • DOI: 10.1186/1756-3305-5-18
  • NAID: 120006522746
  • Peer Reviewed
• [Journal Article] Serological and molecular tools to detect neurologic parasitic zoonoses in rural Cameroon (2011)
  • Author(s): Nkouawa A, Sako Y, Moyou-Somo R, Ito A.
  • Journal Title: Southeast Asian J Trop Med Public Health Volume: 42(6) Pages: 1365-74
• [Journal Article] Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): an inference from nuclear protein-coding genes (2011)
  • Author(s): Knapp J, Nakao M, Yanagida T, Okamoto M, Saarma U, Lavikainen A, Ito A.
  • Journal Title: Mol Phylogenet Evol Volume: 61(3) Pages: 628-38
• [Journal Article] Immunochromatographic test with recombinant Em18 antigen for the follow-up study of alveolar echinococcosis (2011)
  • Author(s): Sako Y, Tappe D, Fukuda K, Kobayashi Y, Itoh S, Frosch M, Gruner B, Kern P, Ito A.
  • Journal Title: Clin Vaccine Immunol Volume: 18(8) Pages: 1302-5
• [Journal Article] The first workshop towards the control of cestode zoonoses in Asia and Africa (2011)
  • Author(s): Ito A, Okamoto M, Li T, Wandra T, Dharmawan NS, Swastika KI, Dekumyoy P, Kusolsuk T, Davvajav A, Davaasuren A, Dorjsuren T, Mekonnen SM, Negasi ZH, Yanagida T, Sako Y, Nakao M, Nakaya K, Lavikainen AJ, Nkouawa A, Mohammadzadeh T.
  • Journal Title: Parasit Vectors Volume: 21
• [Journal Article] Echinococcus multilocularis: identification and functional characterization of cathepsin B-like peptidases from metacestode (2011)
  • Author(s): Sako Y, Nakaya K, Ito A.
  • Journal Title: Exp Parasitol Volume: 127(3) Pages: 693-701
  • NAID: 120005300125
• [Presentation] チベット包虫Echinococcus shiquicusの凍結保存 (2013)
  • Author(s): 中谷和宏、柳田哲矢、迫康仁、中尾稔、伊藤亮
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-29
• [Presentation] 多包虫の凍結保存 (2012)
  • Author(s): 中谷和宏、柳田哲矢、迫康仁、中尾稔、伊藤亮
  • Organizer: 第58回日本寄生虫学会・日本衛生動物学会 北日本支部合同大会
  • Place of Presentation: 北海道旭川市
  • Year and Date: 2012-10-06
• [Presentation] 実験用マウスにおけるチベット包虫の発育(多包虫、単包虫との比較) (2012)
  • Author(s): 中谷和宏、柳田哲矢、迫康仁、中尾稔、伊藤亮
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 兵庫県西宮市
  • Year and Date: 2012-03-24
• [Presentation] チベット包条虫と単包条虫のマウスでの包虫発育 (2011)
  • Author(s): 中谷和宏、柳田哲矢、迫康仁、中尾稔、伊藤亮
  • Organizer: 第57回日本寄生虫学会・日本衛生動物学会 北日本支部合同大会
  • Place of Presentation: 山形県山形市
  • Year and Date: 2011-10-01
• [Presentation] チベット包虫Echinococcus shiquicusの凍結保存
  • Author(s): 中谷和宏
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京都
• [Presentation] 多包虫の凍結保存
  • Author(s): 中谷和宏
  • Organizer: 第58回日本寄生虫学会・日本衛生動物学会 北日本支部合同大会
  • Place of Presentation: 旭川市
• [Presentation] 実験用マウスにおけるチベット包虫の発育（多包虫、単包虫との比較）
  • Author(s): 中谷和宏、柳田哲矢、迫康仁、中尾稔、伊藤亮
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 西宮市 兵庫医科大学
• [Presentation] アジアにおける人獣共通寄生虫病、脳虫症対策に向けた国際共同研究
  • Author(s): 伊藤亮、岡本宗裕、迫康仁、柳田哲矢、中尾稔、中谷和宏
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 西宮市 兵庫医科大学
• [Presentation] チベット包条虫と単包条虫のマウスでの包虫発育
  • Author(s): 中谷和宏、柳田哲矢、迫康仁、中尾稔、伊藤亮
  • Organizer: 第57回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 山形市 山形大学