Generation of a transgenic mouse which can be used for the in vivo detection of CREB phosphorylatio

• Project/Area Number: 23650231
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: University of Toyama
• Principal Investigator: ISHIMOTO Tetsuya 富山大学, 大学院・医学薬学研究部(医学), 助教 (40397170)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: トランスジェニックマウス / ルシフェラーゼ / ＣＲＥＢ / 発光計測

Research Abstract

In 23rdfiscal year, we succeeded in developing the CREB phosphorylation detecting system using anesthesized mouse by adjasting the timing and amount of anesthesia. In this year, we succeeded in observing CREB phosphorylation in non-anesthesized mouse. We confirmed acute adminidtration of Fluoxetine up-regulated the light intensity from mouse brain which indicated the CREB phosphorylation. And we also confirmed the up-regulation of CREB phosphorylation after Fluoxetine treatment by westernblotting.

Research Products

• [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells (2012)
  • Author(s): Ishimoto, T., Mano, H., Ozawa, T., Mori, H.
  • Journal Title: Bioconjug. Chem Volume: 23 Pages: 923-932
  • Peer Reviewed
• [Journal Article] Regulation of gene expression in retrovirus vectors by X-ray and proton beam radiation with artificially constructed promoters. (2012)
  • Author(s): Ogawa, R., Morii, A., Watanabe, A., Cui, Z. G., Kagiya, G.,Fukuda, S., Kume, K., Hasegawa, T., Hatashita, M., Izumi, H., Ishimoto, T.,Feril, L. B.
  • Journal Title: J. Gen. Med. Volume: 14 Pages: 316-327
  • Peer Reviewed
• [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells. (2012)
  • Author(s): T. Ishimoto, H. Mano, T. Ozawa and H. Mori
  • Journal Title: Bioconjugate Chem. Volume: 23 Issue: 5 Pages: 923-932
  • DOI: 10.1021/bc200491j
  • Peer Reviewed
• [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells. (2012)
  • Author(s): Ishimoto T., Mano H., Ozawa T., Mori H.
  • Journal Title: Bioconjugate Chemistry Volume: accepted
  • Peer Reviewed
• [Journal Article] Real-time monitoring of actin polymerization in living cells using split luciferase (2011)
  • Author(s): Ishimoto, T., Ozawa, T., Mori,H.
  • Journal Title: Bioconjug. Chem Volume: 22 Pages: 1136-1144
  • Peer Reviewed
• [Journal Article] Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice. (2011)
  • Author(s): Izumi,H., Ishimoto, T., Yamamoto, H., Nishijo,H., Mori, H.
  • Journal Title: Brain Struct. Funct Volume: 216 Pages: 91-104
  • Peer Reviewed
• [Journal Article] Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms. (2011)
  • Author(s): Taki, H., Gomi, T., Knuckley, B., Thompson, P. R., Vugrek, O., Hirata, K., Miyahara, T., Shinoda, K., Hounoki, H., Sugiyama, E., Usui, I., Urakaze, M., Tobe,K., Ishimoto, T., Inoue, R., Tanaka, A., Mano, H., Ogawa, H., Mori, H.
  • Journal Title: Adv. Biosci. Biotech Volume: 2 Pages: 304-310
  • Peer Reviewed
• [Journal Article] Real-time monitoring of actin polymerization in living cells using split luciferase. (2011)
  • Author(s): Ishimoto T., Ozawa T., Mori H.
  • Journal Title: Bioconjugate Chemistry Volume: 22 Issue: 2 Pages: 1136-1144
  • DOI: 10.1007/s00429-010-0297-2
  • Peer Reviewed
• [Presentation] 生細胞スクリーニングを用いた新規アデニル酸シクラーゼ阻害剤の発見 (2013)
  • Author(s): 真野寛生
  • Organizer: 日本薬理学会
  • Place of Presentation: 福岡
• [Presentation] Analysis of Newly Identified Paranodal Protein BCAS1 in the Schwann cell (2012)
  • Author(s): 石本哲也、井上 蘭、小池正人、内山安男、森 寿
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Detection of CREB activation in living cells using a bioluminescence-based method. (2011)
  • Author(s): Ishimoto T., Mano H., Ozawa T., and Mori H.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 発光蛋白質を用いたCREB活性化の計測 (2011)
  • Author(s): 石本哲也,眞野寛生,小澤岳昌,森 寿
  • Organizer: CRESTシンポジウム「新しい計測で生命に迫る 」
  • Place of Presentation: 東京
• [Presentation] Monitoring of CREB phosphorylation in live cells using split luciferase technique (2011)
  • Author(s): Ishimoto T., Mano H., Ozawa T., and Mori H
  • Organizer: Society For Neuroscience's 41th annual meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] 生物発光を用いたCREBリン酸化検出 (2011)
  • Author(s): 石本哲也,眞野寛生,小澤岳昌,森 寿
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横 浜
• [Presentation] Detection of CREB phosphorylation in live cells using a bioluminescence based method. Ishimoto T (2011)
  • Author(s): Mano H., Ozawa T., and Mori H.
  • Organizer: The 6th International conference of Neurons and Brain Diseases
  • Place of Presentation: Toyama.
• [Presentation] Detection of CREB phosphorylation in live cells using a bioluminescence based method. (2011)
  • Author(s): Ishimoto T., Mano H., Ozawa T., and Mori H.
  • Organizer: The 6th International conference of Neurons and Brain Diseases
  • Place of Presentation: Toyama
• [Presentation] Monitoring of CREB phosphorylation in live cells using split luciferase technique. (2011)
  • Author(s): Ishimoto T., Mano H., Ozawa T., and Mori H.
  • Organizer: Society For Neuroscience's 41th annual meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] 生物発光を用いたCREBリン酸化検出 (2011)
  • Author(s): 石本 哲也, 眞野 寛生, 小澤 岳昌, 森 寿
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜