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Generation of a transgenic mouse which can be used for the in vivo detection of CREB phosphorylatio

Research Project

Project/Area Number 23650231
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionUniversity of Toyama

Principal Investigator

ISHIMOTO Tetsuya  富山大学, 大学院・医学薬学研究部(医学), 助教 (40397170)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsトランスジェニックマウス / ルシフェラーゼ / CREB / 発光計測
Research Abstract

In 23rdfiscal year, we succeeded in developing the CREB phosphorylation detecting system using anesthesized mouse by adjasting the timing and amount of anesthesia. In this year, we succeeded in observing CREB phosphorylation in non-anesthesized mouse. We confirmed acute adminidtration of Fluoxetine up-regulated the light intensity from mouse brain which indicated the CREB phosphorylation. And we also confirmed the up-regulation of CREB phosphorylation after Fluoxetine treatment by westernblotting.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (10 results)

  • [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells2012

    • Author(s)
      Ishimoto, T., Mano, H., Ozawa, T., Mori, H.
    • Journal Title

      Bioconjug. Chem

      Volume: 23 Pages: 923-932

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of gene expression in retrovirus vectors by X-ray and proton beam radiation with artificially constructed promoters.2012

    • Author(s)
      Ogawa, R., Morii, A., Watanabe, A., Cui, Z. G., Kagiya, G.,Fukuda, S., Kume, K., Hasegawa, T., Hatashita, M., Izumi, H., Ishimoto, T.,Feril, L. B.
    • Journal Title

      J. Gen. Med.

      Volume: 14 Pages: 316-327

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells.2012

    • Author(s)
      T. Ishimoto, H. Mano, T. Ozawa and H. Mori
    • Journal Title

      Bioconjugate Chem.

      Volume: 23 Issue: 5 Pages: 923-932

    • DOI

      10.1021/bc200491j

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells.2012

    • Author(s)
      Ishimoto T., Mano H., Ozawa T., Mori H.
    • Journal Title

      Bioconjugate Chemistry

      Volume: accepted

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-time monitoring of actin polymerization in living cells using split luciferase2011

    • Author(s)
      Ishimoto, T., Ozawa, T., Mori,H.
    • Journal Title

      Bioconjug. Chem

      Volume: 22 Pages: 1136-1144

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice.2011

    • Author(s)
      Izumi,H., Ishimoto, T., Yamamoto, H., Nishijo,H., Mori, H.
    • Journal Title

      Brain Struct. Funct

      Volume: 216 Pages: 91-104

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms.2011

    • Author(s)
      Taki, H., Gomi, T., Knuckley, B., Thompson, P. R., Vugrek, O., Hirata, K., Miyahara, T., Shinoda, K., Hounoki, H., Sugiyama, E., Usui, I., Urakaze, M., Tobe,K., Ishimoto, T., Inoue, R., Tanaka, A., Mano, H., Ogawa, H., Mori, H.
    • Journal Title

      Adv. Biosci. Biotech

      Volume: 2 Pages: 304-310

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time monitoring of actin polymerization in living cells using split luciferase.2011

    • Author(s)
      Ishimoto T., Ozawa T., Mori H.
    • Journal Title

      Bioconjugate Chemistry

      Volume: 22 Issue: 2 Pages: 1136-1144

    • DOI

      10.1007/s00429-010-0297-2

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 生細胞スクリーニングを用いた新規アデニル酸シクラーゼ阻害剤の発見2013

    • Author(s)
      真野寛生
    • Organizer
      日本薬理学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of Newly Identified Paranodal Protein BCAS1 in the Schwann cell2012

    • Author(s)
      石本哲也、井上 蘭、小池正人、内山安男、森 寿
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of CREB activation in living cells using a bioluminescence-based method.2011

    • Author(s)
      Ishimoto T., Mano H., Ozawa T., and Mori H.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 発光蛋白質を用いたCREB活性化の計測2011

    • Author(s)
      石本哲也,眞野寛生,小澤岳昌,森 寿
    • Organizer
      CRESTシンポジウム「新しい計測で生命に迫る 」
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Monitoring of CREB phosphorylation in live cells using split luciferase technique2011

    • Author(s)
      Ishimoto T., Mano H., Ozawa T., and Mori H
    • Organizer
      Society For Neuroscience's 41th annual meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] 生物発光を用いたCREBリン酸化検出2011

    • Author(s)
      石本哲也,眞野寛生,小澤岳昌,森 寿
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横 浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of CREB phosphorylation in live cells using a bioluminescence based method. Ishimoto T2011

    • Author(s)
      Mano H., Ozawa T., and Mori H.
    • Organizer
      The 6th International conference of Neurons and Brain Diseases
    • Place of Presentation
      Toyama.
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of CREB phosphorylation in live cells using a bioluminescence based method.2011

    • Author(s)
      Ishimoto T., Mano H., Ozawa T., and Mori H.
    • Organizer
      The 6th International conference of Neurons and Brain Diseases
    • Place of Presentation
      Toyama
    • Related Report
      2011 Research-status Report
  • [Presentation] Monitoring of CREB phosphorylation in live cells using split luciferase technique.2011

    • Author(s)
      Ishimoto T., Mano H., Ozawa T., and Mori H.
    • Organizer
      Society For Neuroscience's 41th annual meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] 生物発光を用いたCREBリン酸化検出2011

    • Author(s)
      石本 哲也, 眞野 寛生, 小澤 岳昌, 森 寿
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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