| Project/Area Number |
23650244
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
ETO Tomoo 公益財団法人実験動物中央研究所, その他部局等, 研究員 (30370175)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 組織・臓器の保存 / 実験動物 / 保存 / 超低温保存 / Cryobiology / 卵巣 / 精巣 / 電圧 / 生殖工学 |
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| Research Abstract |
We have studied the high voltage cryopreservation method of organ or tissue. In the experiment, used the ovaries or testes of the mouse. And egg yolk was also frozen. The organs of mice were cooled to -15 or -30 degree C with application of 5000 V, and then placed in liquid nitrogen for cryopreservation. For thawing, the organs were placed at room temperature and a voltage of 5,000 V was applied. Testes or ovaries were transplanted into the recipient after thawing. It was not possible to obtain gametes from the ovaries or testes were cryopreserved. However, part of the organization were alive. After thawing, egg yolk that contains the irreversible protein at low temperature remained to be packed. Results of this study, the possibility of organ preservation has been suggested. However, can not preservation of complete traits.
The experiments of organ preservation in the future, it is necessary to study the protection of intracellular material.
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