Identification of regeneration promoting factors

• Project/Area Number: 23650433
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied health science
• Research Institution: Osaka University
• Principal Investigator: FUKADA So-ichiro 大阪大学, 薬学研究科(研究院), 研究員 (20432445)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨格筋 / 加齢 / 幹細胞 / 交際情報交流

Research Abstract

Skeletal muscle has great regenerative potential that is dependent on muscle stem cells, also known as satellite cells. Intriguingly, the regenerative potential is dependent on mouse strain. We found that C57BL/6 strain has great regenerative potential, but DBA/2 strain shows inferior potential. In order to identify the 'factor' which regulates regenerative capacity, microarray analyses were performed. Sixty-nine genes were highly expressed in C57BL/6-derived myoblasts compared with DBA/2-derived myoblasts. Among 69 genes, 9 genes were also highly expressed in C57BL/6-derived mesenchymal progenitors that in DBA/2-derived them. Further analyses will lead to identify the regeneration promoting factor which might be a new therapeutic strategy for skeletal muscle disorders.

Research Products

• [Journal Article] Adult stem cell and mesenchymal progenitor theories of aging (2014)
  • Author(s): Fukada, S. Ma, Y. Uezumi, A.
  • Journal Title: Front. Cell Dev. Bio. Volume: in press
  • Peer Reviewed
• [Journal Article] Isolation, characterization, and molecular regulation of muscle stem cells (2013)
  • Author(s): Fukada S, Ma Y., Ohtani T, Watanabe Y, Murakami S., Yamaguchi M
  • Journal Title: Frontiers in Physiology Volume: 4 Pages: 317-317
• [Journal Article] Impaired viability of muscle precursor cells in muscular dystrophy with glycosylation defects and amelioration of its severe phenotype by limited gene expression (2013)
  • Author(s): Kanagawa M, Yu C, Ito C, Fukada S, Hozoji-Inada M, Chiyo T, Kuga A, Matuo M, Sato K, Yamaguchi M, Ito T, Katanosaka Y, Miyagoe-Suzuki Y, Naruse K, Kobayashi K, Okada T, Takeda S, Toda T
  • Journal Title: Human Molecular Genetics Volume: 22(15) Pages: 3003-3015
• [Journal Article] Imatinib attenuates dystrophic condition in severe mouse dystrophy and inhibits both proliferation and fibrosis-marker expression in muscle mesenchymal progenitors (2013)
  • Author(s): Ito T, Ogawa R, Uezumi A, Watanabe Y, Ohtani T, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H, Fukada S
  • Journal Title: Neuromuscular Disorders Volume: 23 Pages: 349-56
• [Journal Article] 転写因子制御機構からの筋発生・筋再生の解明, 整形・災害外科 (2013)
  • Author(s): 山口賢彦, 大谷拓史, 深田宗一朗
  • Journal Title: 臨時増刊号 Volume: 56 (5) Pages: 683-688
• [Journal Article] 筋サテライト細胞の維持機構 (2013)
  • Author(s): 渡邉洋子, 山口賢彦, 深田宗一朗
  • Journal Title: 生体の科学 Volume: 64 (2) Pages: 111-116
• [Journal Article] Isolation, characterization, and molecular regulation of muscle stem cells (2013)
  • Author(s): Fukada, S. Ma, Y. Ohtani, T. Watanabe, Y. Murakami, S. Yamaguchi, M.
  • Journal Title: Front Physiol Volume: 4 Pages: 1-16
  • DOI: 10.3389/fphys.2013.00317
  • Peer Reviewed
• [Journal Article] Impaired viability of muscle precursor cells in muscular dystrophy with glycosylation defects and amelioration of its severe phenotype by limited gene expression (2013)
  • Author(s): Kanagawa M, Yu C, Ito C, Fukada S, Hozoji-Inada M, Chiyo T, Kuga A, Matuo M, Sato K, Yamaguchi M, Ito T, Katanosaka Y, Miyagoe-Suzuki Y, Naruse K, Kobayashi K, Okada T, Takeda S, Toda T
  • Journal Title: Human Molecular Genetic Volume: in press
  • Peer Reviewed
• [Journal Article] Imatinib attenuates dystrophic condition in severe mouse dystrophy and inhibits both proliferation and fibrosis-marker expression in muscle mesenchymal progenitors (2013)
  • Author(s): Ito T, Ogawa R, Uezumi A, Watanabe Y, Ohtani T, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H, Fukada S
  • Journal Title: Neuromuscular Disorders. Volume: 23 Issue: 4 Pages: 349-56
  • DOI: 10.1016/j.nmd.2012.10.025
  • Peer Reviewed
• [Journal Article] 筋サテライト細胞の維持機構 (2013)
  • Author(s): 渡邉洋子，山口賢彦，深田宗一朗
  • Journal Title: 生体の科学 Volume: 印刷中
• [Journal Article] 転写因子制御機構からの筋発生・筋再生の解明 (2013)
  • Author(s): 山口賢彦，大谷拓史，深田宗一朗
  • Journal Title: 整形・災害外科 臨時増刊号 Volume: 印刷中
• [Journal Article] Calcitonin receptor and Odz4 are differently expressed in Pax7-positive cells during skeletal muscle regeneration (2012)
  • Author(s): Yamaguchi M, Ogawa R, Watanabe Y, Uezumi A, Miyagoe-Suzuki Y, Tsujikawa K, Yamamoto H, Takeda S, Fukada S
  • Journal Title: J. Mol. Histol Volume: 43 Pages: 581-87
• [Journal Article] Calcitonin receptor and Odz4 are differently expressed in Pax7-positive cells during skeletal muscle regeneration (2012)
  • Author(s): Yamaguchi M, Ogawa R, Watanabe Y, Uezumi A, Miyagoe-Suzuki Y, Tsujikawa K, Yamamoto H, Takeda S, Fukada S
  • Journal Title: J. Mol. Histol. Volume: 43 Issue: 5 Pages: 581-7
  • DOI: 10.1007/s10735-012-9421-3
  • Peer Reviewed
• [Journal Article] Adult stem cell or mesenchymal progenitors theories for aging
  • Author(s): Fukada S, Ma Y., and Uezumi A
  • Journal Title: Frontiers in Cell and Development
• [Presentation] ホルモン受容体による骨格筋幹細胞維持メカニズム (2012)
  • Author(s): 深田宗一朗
  • Organizer: 第35回日本分子生物学会 ワークショップ W66骨格筋幹細胞研究の最前線
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] 骨格筋幹細胞から筋疾患治療開発へのアプローチ (2012)
  • Author(s): 深田宗一朗
  • Organizer: 東京女子医科大学テニュアトラック教員支援セミナー
  • Place of Presentation: 東京女子医科大学
  • Year and Date: 2012-11-02
• [Presentation] Molecular regulation of muscle stem cells by 'quiescence genes' (2012)
  • Author(s): So-ichiro Fukada
  • Organizer: 9th Japanese-French Symposium for 'muscular dystrophy'
  • Place of Presentation: JA共済ビルカンファレンス
  • Year and Date: 2012-09-08
• [Presentation] Calcitonin receptor regulates muscle satellite cells (2012)
  • Author(s): So-ichiro Fukada, Masahiko Yamaguchi, Yoko Watanabe, Takuji Ohtani
  • Organizer: Oral presentation , FASEB Summer Research Conferences
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2012-08-14
• [Presentation] Muscle regeneration and muscle stem cells (2012)
  • Author(s): So-ichiro Fukada, Bad Nauheim
  • Organizer: Max-Planck Institute, ECCPS Mini symposium
  • Place of Presentation: Germany
  • Year and Date: 2012-05-31
• [Presentation] Muscle regeneration and muscle stem cells
  • Author(s): So-ichiro Fukada
  • Organizer: Max-Planck Institute, ECCPS Mini symposium,
  • Place of Presentation: Bad Nauheim, Germany
  • Invited
• [Presentation] Calcitonin receptor regulates muscle satellite cells
  • Author(s): So-ichiro Fukada, Masahiko Yamaguchi, Yoko Watanabe, Takuji Ohtani
  • Organizer: FASEB Summer Research Conferences
  • Place of Presentation: Lucca, Italy,
• [Presentation] 骨格筋幹細胞から筋疾患治療開発へのアプローチ
  • Author(s): 深田宗一朗
  • Organizer: 東京女子医科大学 テニュアトラック教員支援セミナー
  • Place of Presentation: 東京女子医大
  • Invited
• [Presentation] Molecular regulation of muscle stem cells by ‘quiescence genes’
  • Author(s): So-ichiro Fukada
  • Organizer: 9th Japanese-French Symposium for ‘muscular dystrophy’
  • Place of Presentation: JA共済ビルカンファレンス
  • Invited
• [Presentation] ホルモン受容体による骨格筋幹細胞維持メカニズム
  • Author(s): 深田宗一朗
  • Organizer: 第３５回日本分子生物学会 ワークショップ W66
  • Place of Presentation: 福岡国際会議場
  • Invited
• [Book] Regulation of muscle stem cell quiescence and undifferentiated state: The role of Hesr1 and Hesr3, Tumor Dormancy, Quiescence, and Tissue Senescence (eds. M.A. Hayat) (2013)
  • Author(s): Yamaguchi M and Fukada S
  • Publisher: Springer
• [Book] Tumor Dormancy, Quiescence, and Tissue Senescence (eds. M.A. Hayat): Regulation of muscle stem cell quiescence and undifferentiated state: The role of Hesr1 and Hesr3 (2013)
  • Author(s): Yamaguchi M, Fukada S
  • Publisher: Springer