Analysis of structural regulation of plasma and nuclear membranes in cancer cells by Wnt5a-Ror2 signaling

• Project/Area Number: 23650595
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Kobe University
• Principal Investigator: MINAMI Yasuhiro 神戸大学, 大学院・医学研究科, 教授 (70229772)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Wnt5a / Ror2 / 低分子量Gタンパク質 / 核膜形態 / がん細胞の浸潤 / 細胞膜形態 / 核膜タンパク質 / タンパク質分解

Research Abstract

In this study it has been found that Rif, a member of the Rho-family of small G-proteins, and emerin, an integral nuclear membrane protein, play a critical role in the morphological regulation of plasma membrane and nuclear membrane by Wnt5a-Ror2 signaling. Furthermore, it has been indicated that Rif and its putative upstream regulator, Rap1GDS1, are involved in cancer cell proliferation in addition to their role in filopodia formation of cancer cells.

Research Products

• [Journal Article] Activation of Wnt5a-Ror2 signaling associated with epithelial-to-mesenchymal transition (EMT) of tubular epithelial cells during renal fibrosis. (2013)
  • Author(s): Li, X., Yamagata, K., Nishita, M., Endo, M., Arfian, N., Rikitake, Y., Emoto, N., Hirata, K., Tanaka, Y., Minami, Y.
  • Journal Title: Genes Cells Volume: 掲載確定・印刷中
  • Peer Reviewed
• [Journal Article] Activation of Wnt5a-Ror2 signaling associated with epithelial-to-mesenchymal transition (EMT) of tubular epithelial cells during renal fibrosis. (2013)
  • Author(s): Li, X.
  • Journal Title: Genes Cells Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Analysis of Wnt/planar cell polarity pathway in cultured cells (2012)
  • Author(s): 西田満
  • Journal Title: Methods in Molecular Biology Volume: 839 Pages: 201-214
  • DOI: 10.1007/978-1-61779-510-7_16
  • ISBN: 9781617795091, 9781617795107
• [Journal Article] Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase(MMP-13) expression (2012)
  • Author(s): Yamagata K, Li X, Ikegaki S, Oneyama C, Okada M, Nishita M, Minami Y
  • Journal Title: J Biol Chem Volume: 287(2) Issue: 2 Pages: 1588-99
  • DOI: 10.1074/jbc.m111.315127
  • Peer Reviewed
• [Journal Article] Ror-family receptor tyrosine kinases regulate maintenance of neural progenitor cells in the developing neocortex (2012)
  • Author(s): Endo M, Doi R, Nishita M, Minami Y
  • Journal Title: Journal of Cell Science Volume: (印刷中)(掲載確定) Pages: 2017-2029
  • DOI: 10.1242/jcs.097782
  • Peer Reviewed
• [Journal Article] Wnt5a-Ror2 signaling between osteoblasts and osteoclast precursors enhances osteoclastogenesis (2012)
  • Author(s): Maeda, K., Kobayashi, Y., Udagawa, N., Uehara, S., Ishihara, A., Mizoguchi, T., Kikuchi, Y., Takada, I., Kato, S., Kani, S., Nishita, M., Marumo, K., Martin, T.J., Minami, Y., Takahashi, N.
  • Journal Title: Nat.Med. Volume: (印刷中) Issue: 3 Pages: 405-12
  • DOI: 10.1038/nm.2653
  • Peer Reviewed
• [Journal Article] 非古典的Wntシグナルによる極性と幹細胞性維持の制御 (2012)
  • Author(s): 遠藤光晴
  • Journal Title: 生体の科学（特集：細胞極性の制御） Volume: 63 Pages: 224-228
• [Journal Article] Analysis of Wnt/Planar cell polarity pathway in cultured cells. (2012)
  • Author(s): Endo, M., Nishita, M., Minami, Y.
  • Journal Title: Methods Mol. Biol. Volume: 839 Pages: 201-214
• [Journal Article] Critical role of Wnt5a-Ror2 signaling in motility and invasiveness of epidermoid carcinoma cells following Snail-mediated epithelial-mesenchymal transition (2011)
  • Author(s): Ren, D., Minami, Y., Nishita, M.
  • Journal Title: Genes Cells Volume: 16 Issue: 3 Pages: 304-315
  • DOI: 10.1111/j.1365-2443.2011.01487.x
  • Peer Reviewed
• [Journal Article] Non-canonical Wnt signaling mediates androgen-dependent tumor growth in a mouse model of prostate cancer (2011)
  • Author(s): Takahashi, S., Watanabe, T., Okada, M., Inoue, K., Ueda, T., Takada, I., Watabe, T., Yamamoto, Y., Fukuda, T., Nakamura, T., Akimoto, C., T., Fujimura, T., Hoshino, M., Imai, Y., Metzger, D., Miyazono, K., Minami, Y., Chambon, P., Kitamura, T., Matsumoto, T., Kato, S.
  • Journal Title: Proc. Natl. Acad. Sci Volume: Vol. 108 Issue: 12 Pages: 4938-4943
  • DOI: 10.1073/pnas.1014850108
  • Peer Reviewed
• [Journal Article] Non-canonical Wnt signaling mediates androgen-dependent tumor growth in a mouse model of prostate cancer. (2011)
  • Author(s): Takahashi, S., 他13名, Metzger, D., Miyazono, K., Minami, Y., Chambon, P., Kitamura, T., Matsumoto, T., Kato, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 4938-4943
  • Peer Reviewed
• [Presentation] 骨格筋分化・再生モデルを用いたRorシグナルの機能解析 (2012)
  • Author(s): 土井亮介、遠藤光晴、南康博
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ
  • Year and Date: 2012-12-14
• [Presentation] マウス腎臓発生におけるWnt5a-Ror2シグナルの役割 (2012)
  • Author(s): 西田満、喬森、宮本真理、山田真紀子、大谷浩、Christine Hartmann、西中村隆一、南康博
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 第35回日本分子生物学会年会
  • Year and Date: 2012-12-14
• [Presentation] Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase (MMP)-13 expression (2011)
  • Author(s): Kaoru Yamagata, Shunkichi Ikegaki, Xin Li, Yasuhiro Minami
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] 骨格筋分化・再生モデルを用いたRorシグナルの機能解析
  • Author(s): 土井亮介
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ
• [Presentation] マウス腎臓発生におけるWnt5a-Ror2シグナルの役割
  • Author(s): 西田満
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ
• [Presentation] Wnt5a-Ror signaling under physiological and pathological conditions
  • Author(s): 南康博
  • Organizer: 第3回GCOE国際シンポジウム
  • Place of Presentation: 神戸ポートピアホテル
  • Invited
• [Book] The Receptor Tyrosine Kinase Handbook (2013)
  • Author(s): Endo, M., Nishita, M., Doi, R., Minami, Y.
  • Publisher: Springer Science(掲載確定・印刷中)
• [Book] The Receptor Tyrosine Kinase Handbook (chapter: Ror receptor family) (2013)
  • Author(s): Endo, M
  • Publisher: Springer Science
• [Book] Progenitor Cells: Biology, Characterization, and Potential Clinical Applications (2013)
  • Author(s): Endo, M
  • Publisher: Nova Science Publishers, Inc.
• [Book] The Receptor Tyrosine Kinase Handbook (chapter: Ror receptor family) (2012)
  • Author(s): Endo, M., Nishita, M., Doi, R., Minami, Y.
  • Publisher: Springer Science
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/medzoo/
• [Remarks] 神戸大学大学院医学研究科 細胞生理学分野HP
  • URL: http://www.med.kobe-u.ac.jp/medzoo/
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/medzoo/