Development of novel drug delivery system for micro RNA nucleic biomedicine using exosome from human cultured cells

• Project/Area Number: 23650626
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Clinical oncology
• Research Institution: Hiroshima University
• Principal Investigator: TAHARA Hidetoshi 広島大学, 大学院・医歯薬学総合研究科, 教授 (00271065)
• Co-Investigator(Renkei-kenkyūsha): OCHIYA Takahiro 独立行政法人国立がん研究センター, がん転移研究室, 室長 (60192530) ; KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: エクソソーム / マイクロRNA / ドラッグデリバリーシステム / がん / DDS

Research Abstract

We have developed Cd44-GFP expressing cells including normal and cancer cell lines to visualized exosomes. We also purified CD44-GFP labeled exosomes form those cells. We confirmed that CD44-GFP labeled exosomes cargo microRNA by adding these exosomes to the other cells. These tools should be useful for exosome derived DDS technology.

Research Products

• [Journal Article] miR-22 represses cancer progressionby inducing cellular senescence (2011)
  • Author(s): D. Xu, F. Takeshita, Y. Hino, S. Fukunaga, Y. Kudo, A. Tamaki, J. Matsunaga, R. Takahashi, T. Takata, A. Shimamoto, T. Ochiya, H. Tahara
  • Journal Title: J. Cell Biology Volume: 193 Pages: 409-442
  • Peer Reviewed
• [Journal Article] Two unrelatedpatients with MRE11A mutationsand Nijmegen breakage syndrome-like severe microcephaly (2011)
  • Author(s): Y. Matsumoto, T. Miyamoto, H. Sakamoto, H. Izumi, Y. Nakazawa, T. Ogi, H. Tahara, S. Oku, A. Hiramoto, T. Shiiki, Y. Fujisawa, H. Ohashi, Y. Sakemi, S. Matsuura
  • Journal Title: DNA Repair Volume: 10 Pages: 314-321
  • Peer Reviewed
• [Journal Article] miR-22 represses cancer progression by inducing cellular senescence (2011)
  • Author(s): Xu D, Takeshita F,他 10人
  • Journal Title: J. Cell Biol. Volume: 193 Issue: 2 Pages: 409-424
  • DOI: 10.1083/jcb.201010100
  • Peer Reviewed
• [Journal Article] Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly. (2011)
  • Author(s): 松本 祥幸
  • Journal Title: DNA Repair Volume: 10 Issue: 3 Pages: 314-321
  • DOI: 10.1016/j.dnarep.2010.12.002
  • Peer Reviewed
• [Presentation] Micro RNA profiling in senescent associated exosome (2011)
  • Author(s): 中村亜由美、岡田恵、小坂展慶、落谷孝広、田原栄俊
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-24
• [Presentation] Visualization of senescence secreted exosome. (2011)
  • Author(s): 岡田恵、中村亜由美、小坂展慶、落谷孝広、田原栄俊
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] Visualization of senescence secreted exosome (2011)
  • Author(s): 岡田恵、中村亜由美、小坂展慶、落谷孝広、田原栄俊
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] MicroRNA profiling in senescent associated exosome (2011)
  • Author(s): 中村亜由美、岡田恵、小坂展慶、落谷孝広、田原栄俊
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Senescent associated microRNAs analysis inexosomes (2011)
  • Author(s): Ayumi Nakamura, Nobuyoshi Kosaka, Aya Tamaki, Megumi Okada, TakahiroOchiya, Hidetoshi Tahara
  • Organizer: Exosome and Microvesice
  • Place of Presentation: Orland, USA
  • Year and Date: 2011-10-16
• [Presentation] Senescent associated microRNAs analysis in exosomes (2011)
  • Author(s): Ayumi Nakamura, Nobuyoshi Kosaka, Aya Tamaki, Megumi Okada, Takahiro Ochrya, Hidetoshi Tahara
  • Organizer: Exosome and Microvesice
  • Place of Presentation: Orland, USA
  • Year and Date: 2011-10-16