| Project/Area Number |
23650629
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical oncology
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| Research Institution | Miyagi Cancer Center Research Institute |
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Principal Investigator |
SATO Ikuro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), ティッシュバンクセンター, センター長 (50225918)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Shigemi 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (80600006)
TANUMA Nobuhiro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 主任研究員 (40333645)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 代謝 / 解糖系 / ワールブルグ効果 / PKM / スプライシング / 分子標的治療 / 代謝異常 |
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| Research Abstract |
A glycolytic enzyme, pyruvate kinase M (PKM), has been suggested to play important roles in altered metabolism in cancer cells. In this study, we analyzed splicing regulation of the PKM gene in tumor. Analysis using clinical specimens revealed that pre-mRNA splicing of PKM gene switches from M1- to M2-type in most case of various human cancers. In addition, we developed a reporter gene enabling us to detect PKM conversion in living cells.
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