Non-linear DNA repair responses caused by interference between competitive repair pathways
| Project/Area Number |
23651049
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
YOKOYA Akinari 独立行政法人日本原子力研究開発機構, 原子力科学研究部門・先端基礎研究センター, 研究主席 (10354987)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | クラスターDNA損傷 / DNA修復応答 / 酵素反応干渉 / 非線形性 / 塩基除去修復 / Nth / Fpg / 炭素イオンビーム / DNA変性とアニーリング / 放射線、X線、粒子線 / 酵素作用機序 / DNA変性 / 放射線 / DNA損傷 / 干渉 / グリコシレース / プラスミドDNA / DNA2本鎖切断 |
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| Research Abstract |
Ionizing radiation induce localized DNA damage composed of various base lesions (clustered damage). It is expected that damage repair enzymes would be interfered at the localized area on DNA. In this study we aimed to clarify whether the variation of enzymatic processes modify each enzymatic activities at clustered damage site. DNA samples exposed to X-rays were treated with two base excision repair enzymes, namely Nth and Fpg. The enzymes were added sequentially or simultaneously. Contrast to our initial expectation, change of the order of the enzymatic treatments did not affect the activities of the enzymes. This result suggests that, even in a cluster damage site, each base lesions substantially separated not to induce interference between each enzymatic pathways.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation2013
Author(s)
Shiina, T., Watanabe, R., Shiraishi, I., Suzuki, M., Sugaya, Y., Fujii, K. and Yokoya, A
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Journal Title
Radiat. Environ. Biophys
Volume: 52
Pages: 99-112
Related Report
Peer Reviewed
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[Journal Article] Yield of single-, double-strand breaks and nucleobase lesions in fully hydrated plasmid DNA films irradiated with high-LET charged particles2012
Author(s)
Ushigome, T., Shikazono, N., Fujii, K., Watanabe, R., Suzuki, M., Tsuruoka, C., Tauchi, H. and Yokoya, A
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Journal Title
Radiat. Res
Volume: 177
Pages: 614-627
Related Report
Peer Reviewed
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[Presentation] Molecular and Cellular Effect of Ionizing Radiation2013
Author(s)
Yokoya, A., Oka, T., Fujii, K., Shiina, T., Sugaya, Y., Shiraishi, I., Urushibara, A., Narita1, A., Noguchi, M. and Watanabe, R
Organizer
The 16th SANKEN International The 11th SANKEN Nanotechnology Symposium
Place of Presentation
Osaka, Japan
Related Report
Invited
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[Book] Chapter 20. Spectroscopic study of radiation-induced DNA lesions and their susceptibility to enzymatic repair. In : Charged particle and photon interactions with matter-recent advances, applications and interfaces, Eds., Y. Hatano, Y. Katsumura, and A. Mozumder2011
Author(s)
Yokoya, A., Fujii, K., Shikazono, N. and Ukai, M
Publisher
CRC/Taylor & Francis Group, USA
Related Report
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