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Structural and functional studies on Chloroethene sensor kinase and its application for bioremediation

Research Project

Project/Area Number 23651063
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental technology/Environmental materials
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

ODAKA Masafumi  東京農工大学, 大学院・工学研究院, 准教授 (20224248)

Co-Investigator(Renkei-kenkyūsha) YOHDA Masafumi  東京農工大学, 大学院・工学研究院, 教授 (50250105)
NOGUCHI Keiichi  東京農工大学, 学術研究支援総合センター, 准教授 (00251588)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsヒスチジンキナーゼ / PAS ドメイン / 2成分系伝達システム / クロロエテン / バイ オレメディエーション / PASドメイン / バイオレメディエーション
Research Abstract

Chloroethene is one of the most serious pollutants of soil and underground water, and the construction of its detection and removal systems is a very important problem to be solved. In this study, genes of chloroethene sensor kinase were amplified by PCR with a primers set designed based on amino acid sequences of the known chloroethene sensor kinase using a metagenome prepared from a consortium dechlorinating chloroethene to ethene as a template. The obtained sensor kinase was expressed inEscherichia coli and their structures and function were studied.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2012 2011 Other

All Presentation (9 results)

  • [Presentation] Nishimura: Development of bacterial consortia enriched with a novel Dehalococcoides sp. that dechlorinates trichloroethene toethene2012

    • Author(s)
      M. Yohda、M. Kitajima、M. Iwamoto、N.Tamura、J. Shibaski、S. Koike、M
    • Organizer
      8th International ConferenceRemedeation of Chlorinated and Recalsitrant Compounds
    • Place of Presentation
      Monterey, Callifornia ,USA
    • Year and Date
      2012-05-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 正文:Dehalococcoides属細菌由来クロロエテン類センサーキナーゼの発現と機能解析2011

    • Author(s)
      武知文音、荒木智、前田悠貴、北嶋瑞樹、山中保明、野口恵一、尾高雅文、養王田
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京都小金井市、東京農工大学
    • Year and Date
      2011-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 正文:Dehalococcoides属最近由来クロロエテン類センサーキナーゼの発現と機能解析2011

    • Author(s)
      武知文音、荒木智、前田悠貴、北嶋瑞樹、山中保明、野口恵一、尾高雅文、養王田
    • Organizer
      生物工学若手研究者の集い夏のセミナー2011
    • Place of Presentation
      山梨県笛吹市
    • Year and Date
      2011-07-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Dehalococcoides 属細菌由来クロロエテン類センサーキナーゼの発現と機能解析2011

    • Author(s)
      武知文音、荒木智、前田悠貴、北嶋瑞樹、山中保明、野口恵一、尾高雅文、養王田正文
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Dehalococcoides属最近由来クロロエテン類センサーキナーゼの発現と機能解析2011

    • Author(s)
      武知文音、荒木智、前田悠貴、北嶋瑞樹、山中保明、野口恵一、尾高雅文、養王田正文
    • Organizer
      生物工学若手研究者の集い 夏のセミナー2011
    • Place of Presentation
      山梨県春日居
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of Bacterial Consortia Enriched with a Novel Dehalococcoides sp. That Dechlorinates Trichloroethene to Ethene.

    • Author(s)
      M Yohda、M Kitajima、M Iwamoto、N Tamura、J Shibaski、S Koike、M Nishimura
    • Organizer
      8th International Conference Remedeation of Chlorinated and Recalsitrant Compounds
    • Place of Presentation
      Monterey,Callifornia ,USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた Dehalococcoides sp, strain ATV-1のゲノム解析

    • Author(s)
      武知文音、北嶋瑞樹、岩本めぐみ、養王田正文、八木修身、塚原正俊、照屋実盛、喜久里育也、藤森一浩、今田有美、鼠尾まい子、佐藤万仁、佐藤友紀、矢野修一、三輪友希乃、町田雅之、平野隆
    • Organizer
      環境バイオテクノロジー学会 2012年度大会
    • Place of Presentation
      京都大学宇治キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of Dehalococcoides enriched culture that grows on cis-1,2 dichloroethene and its metagenomic characterizationby 2nd generation sequencer SOLiD

    • Author(s)
      Ayane Takechi、Mizuki Kitajima、Masafumi Yohda
    • Organizer
      生命医薬情報学連合大会
    • Place of Presentation
      Tower Hall Funabori
    • Related Report
      2012 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた Dehalococcoides sp. Strain ATV-1の全ゲノム解析

    • Author(s)
      武知 文音、養王田 正文、矢木 修身、塚原 正俊、照屋 盛実,、喜久利 育也、藤森 一浩、今田 有美、鼠尾まい子、佐藤 万仁、佐藤 友紀、矢野 修一、三輪 友希乃、町田 雅之、平野 隆
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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