Total analysis of adhesive protein genes from mussels.
| Project/Area Number |
23651083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 水中付着生物 / 水生昆虫シルク-セメント / イガイ類接着物質 / 接着物質同定 / 接着機構 / 接着タンパク質遺伝子 / 水生付着生物 / イガイ類 |
|---|
| Research Abstract |
Gene cloning of adhesive proteins from marine and freshwater mussels and one species of aquatic insect have been investigated. In the case of freshwater mussel, Limnoperna fortunei, a fp-2-like protein gene was found as an entire sequence. An enzyme, tyrosinase, was also suggested to be expressed in the mussel foot. The results indicated that freshwater mussels adopts the fp-like protein having higer level os Asp, which imply that electrostatic interactions is one of significant force for protein aggregation in freshwater environments, while presence of DOPA is conserved. The aquatic insect have a giant polypeptide for cement and silk formation. The silk/cement protein has high level of phosphorylated serine residues, and interaction between Ca2+ and the phosphorylated proteins will drive the cement/silk formation.
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Report
(4 results)
Research Products
(141 results)
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[Presentation] Isolation of silk proteins from a caddisfly larva, Stenopsyche marmorata2012
Author(s)
Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Koji Abe, Masuhiro Tsukada and Kimio Hirabayashi
Organizer
Textile Bioengneering Informatics Symposium 2012, Proceedings, pp. 233-242.
Place of Presentation
Shinsnu Univ., Ueda, Japan
Related Report
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[Presentation] Repetitive sequential motifs of underwater silk protein from caddisfly, Stenopsyche marmorata2012
Author(s)
Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Kimio Hirabayashi, Masuhiro Tsukada, and Koji Abe
Organizer
American Chemical Society, National Meething, 2012 Spring. Abstract CD-ROM. Paper Number BIOL163
Place of Presentation
San Diego Convention Center, US
Related Report
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[Presentation] ヒゲナガカワトビケラ絹糸腺のcDNAライブラリーの構築―シルク関連遺伝子の探索2011
Author(s)
石原詩織, 山口裕子, 白 雪, 大川浩作, 新井亮一, 平林公男, 塚田益裕, 阿部康次, 野村隆臣
Organizer
第34回日本分子生物学会, 講演要旨集, Web 掲載
Place of Presentation
横浜 (パシフィコ横浜)
Related Report
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