Development of micro blood-brain barrier

• Project/Area Number: 23651134
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: Gunma University
• Principal Investigator: SATO Kiichi 群馬大学, 大学院・工学研究科, 准教授 (50321906)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: マイクロチップ / 血液脳関門 / 動物実験代替法 / マイクロ流体デバイス / バイオアッセイ

Research Abstract

PDMS microchip with a porous membrane for cell culture support was developed as a micro model of the blood-brain barrier. Vascular endothelial cell line from monkey and human fibroblast-like cell line were co-cultured on both sides of the membrane. Permeability tests of fluorescent molecules were conducted with the microfluidic device. I confirmed the cell sheet in the device acted as a permeability barrier.

Research Products

• [Journal Article] Microcirculation System with a Dialysis Part for Bioassays Evaluating Anticancer Activity and Retention (2013)
  • Author(s): Yuki IMURA, Etsuro YOSHIMURA, Kiichi SATO
  • Journal Title: Analytical Chemistry Volume: 85 Issue: 3 Pages: 1683-1688
  • DOI: 10.1021/ac302938q
  • Peer Reviewed
• [Journal Article] Micro Total Bioassay System for Oral Drugs: Evaluation of Gastrointestinal Degradation, Intestinal Absorption, Hepatic Metabolism, and Bioactivity (2012)
  • Author(s): Yuki IMURA, Etsuro YOSHIMURA, Kiichi SATO
  • Journal Title: Analytical Sciences Volume: 28 Issue: 3 Pages: 197-197
  • DOI: 10.2116/analsci.28.197
  • NAID: 130004442113
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Presentation] Simultaneous measurement of dialysate and sample solution in a microdialysis system (2012)
  • Author(s): Yu Sakuta, Kin-ichi TSUNODA, Kiichi SATO
  • Organizer: RSC Tokyo International Conference
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2012-09-06
• [Presentation] 空気圧駆動ポンプを組み込んだマイクロ透析システムの開発 (2012)
  • Author(s): 作田悠、角田欣一、佐藤記一
  • Organizer: 第25回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 崇城大学(熊本市)
  • Year and Date: 2012-05-18
• [Presentation] マイクロチップを用いたバイオアベイラビリティ評価法の開発 (2011)
  • Author(s): 佐藤記一
  • Organizer: 第25回動物細胞工学シンポジウム
  • Place of Presentation: キャンパス・イノベーションセンター国際会議室(東京)(招待講演)
  • Year and Date: 2011-05-30
• [Presentation] Development of a Micro Cardiovascular System for Bioassay of Anticancer Activity and Renal Clearance (2011)
  • Author(s): Kiichi SATO, Yuki IMURA, Etsro YOSHIMURA
  • Organizer: IUPAC International Congress on Analytical Sciences 2011 (ICAS2011)
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-05-25
• [Presentation] DEVELOPMENT OF A MICRO CARDIOVASCULAR SYSTEM FOR BIOASSAY OF ANTICANCER ACTIVITY AND RENAL CLEARANCE (2011)
  • Author(s): Kiichi Sato, Yuki Imura, Etsro Yoshimura
  • Organizer: IUPAC International Congress on Analytical Sciences 2011 (ICAS2011)
  • Place of Presentation: 国立京都国際会館
• [Presentation] マイクロチップを用いたバイオアベイラビリティ評価法の開発 (2011)
  • Author(s): 佐藤記一
  • Organizer: 第25回動物細胞工学シンポジウム(招待講演)
  • Place of Presentation: キャンパス･イノベーションセンター国際会議室（東京）
• [Presentation] 空気圧駆動ポンプを組み込んだマイクロ透析システムの開発
  • Author(s): 作田悠
  • Organizer: 第25回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 崇城大学（熊本市）
• [Presentation] Simultaneous measurement of dialysate and sample solution in a microdialysis system
  • Author(s): Yu Sakuta
  • Organizer: RSC Tokyo International Conference
  • Place of Presentation: 幕張メッセ（千葉市）