Molecular mechanism and quantitative analysis of microRNA-mediated gene silencing
| Project/Area Number |
23651200
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UI-TEI Kumiko 東京大学, 大学院・理学系研究科, 准教授 (50213327)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHI Kenji 東京大学, 大学院・理学系研究科, 助教 (50466801)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 機能性 RNA / miRNA silencing / noncoding RNA / miRNA / siRNA / Tm / miTm / 定量的発現調節 / シード領域 / 5'末端 / silencing / 定量的解析 / RISC / mRNA |
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| Research Abstract |
In this study, we performed studies as follows: (1) When exogenous miRNAs (exo-miRNAs) are introduced into cells, endogenous miRNAs (endo-miRNAs) in the RISC may be replaced with exo-miRNAs. We examined the fluctuation of target gene expression of endo-miRNAs response to the introduction of an exo-miRNA using microarray. (2) We identified the crucial factors that determine the efficacy of miRNA-mediated gene silencing, and successfully mathematized the silencing efficacy using values reflecting base-pairing stabilities in miRNA duplex and seed-target duplex.
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Report
(3 results)
Research Products
(76 results)
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[Journal Article] IFPA meeting 2012 workshop report I:Comparative placentation and animal models, advanced techniques in placental histopathology,human pluripotent stem cells as a model for trophoblast differentiation2013
Author(s)
Ackerman WE 4th, Carter AM, De Mestre AM, Golos TG, Jeschke U, Kusakabe K, Laurent LC, Parast MM, Roberts RM, Robinson JM, Rutherford J, Soma H, Takizawa T, Ui-Tei K,Las GE.
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Journal Title
Related Report
Peer Reviewed
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