| Project/Area Number |
23651200
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UI-TEI Kumiko 東京大学, 大学院・理学系研究科, 准教授 (50213327)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHI Kenji 東京大学, 大学院・理学系研究科, 助教 (50466801)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 機能性 RNA / miRNA silencing / noncoding RNA / miRNA / siRNA / Tm / miTm / 定量的発現調節 / シード領域 / 5'末端 / silencing / 定量的解析 / RISC / mRNA |
|---|
| Research Abstract |
In this study, we performed studies as follows: (1) When exogenous miRNAs (exo-miRNAs) are introduced into cells, endogenous miRNAs (endo-miRNAs) in the RISC may be replaced with exo-miRNAs. We examined the fluctuation of target gene expression of endo-miRNAs response to the introduction of an exo-miRNA using microarray. (2) We identified the crucial factors that determine the efficacy of miRNA-mediated gene silencing, and successfully mathematized the silencing efficacy using values reflecting base-pairing stabilities in miRNA duplex and seed-target duplex.
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