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Development ofa newdiagnostic methodby using"diagnosis molecule" for adisease with carbohydrataebnormality

Research Project

Project/Area Number 23651232
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionThe University of Tokyo

Principal Investigator

HATANAKA Kenichi  東京大学, 生産技術研究所, 教授 (70167584)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords診断薬探索 / 糖鎖診断 / 糖鎖 / 生体分子 / 細胞・組織 / 酵素 / 糖 / アルキルラクトシド / 糖脂質 / 細胞 / 診断分子 / 糖鎖生合成 / シアリル化 / GM3 / 診断
Research Abstract

It was shown that saccharide synthesis in the cell can be observed in several hours by adding dodecyl lactoside as " The molecules which diagnose a cell" to the culture medium. When a molecule which affects on saccharide synthesis was added with dodecyl lactoside, change of saccharide synthesis was easily observed. The conjugate of oligosaccharide of glycoprotein and dodecyl chain could be used for the analysis of chain elongation of oligosaccharide. Moreover, it could be done with fluorous glycoside.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (8 results) Book (3 results) Remarks (1 results)

  • [Journal Article] method for the production of sialylpara-globoside2012

    • Author(s)
      Y. Shimura, J. Suzuki, M. C. Z. Kasuya, K. Matsuoka, K. Hatanaka, A novel
    • Journal Title

      Helv. Chim. Acta

      Volume: Vol.95 Pages: 67-75

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Large Scale Biosynthesis of Ganglioside Analogues by RERF-LC- AI Cells Cultured in HYPERFlask2012

    • Author(s)
      Y. Shimura, J. Suzuki, M. Muraoka, M. C. Z. Kasuya, K. Matsuoka, K. Hatanaka
    • Journal Title

      Preparative Biochem. Biotech.

      Volume: Vol.42 Pages: 378-392

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter2012

    • Author(s)
      M. Tojino, M. Mori, M. C. Z. Kasuya, K. Hatanaka, A. Kawaguchi, K. Nagata, T. Shirai, M. Mizuno
    • Journal Title

      Bioorg. Med. Chem. Lett

      Volume: Vol.22 Issue: 2 Pages: 1251-1254

    • DOI

      10.1016/j.bmcl.2011.11.057

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of passage number on glycosylation of alkyl lactosides by MDCK cells2012

    • Author(s)
      Y. Shimura, J. Suzuki, M. Muraoka, M. C. Z. Kasuya, K. Matsuoka, K. Hatanaka
    • Journal Title

      J. Biosci. Bioeng

      Volume: Vol.114 Issue: 5 Pages: 552-555

    • DOI

      10.1016/j.jbiosc.2012.06.007

    • NAID

      110009553591

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the Hydrophobicity of Perfluoroalkyl Chains in Amphiphilic Compounds That Are Incorporated into Cell Membrane2011

    • Author(s)
      M. C. Kasuya, S. Nakano, R. Katayama, and K. Hatanaka
    • Journal Title

      J. Fluorine Chem

      Volume: Vol.132 Issue: 3 Pages: 202-206

    • DOI

      10.1016/j.jfluchem.2011.01.004

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physicochemical Derivery of Amphiphilic Copolymers to Specific Organelles2011

    • Author(s)
      R. Kojima, M. C. Z. Kasuya, K. Ishihara, K. Hatanaka
    • Journal Title

      Polym. J

      Volume: Vol.43 Issue: 8 Pages: 718-722

    • DOI

      10.1038/pj.2011.49

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] K. Hatanaka,an Efficient Method in Searching for Glycosylation Regulating Compounds2012

    • Author(s)
      Y. Nishiyama,
    • Organizer
      26 International Carbohydrate Symposium
    • Place of Presentation
      Madrid (Spain)
    • Year and Date
      2012-07-24
    • Related Report
      2012 Final Research Report
  • [Presentation] K. Hatanaka, Synthesis of Hybrid Glyco- peptide2012

    • Author(s)
      M. Mizuno, K. Koichi, M. C. Z. Kasuya
    • Organizer
      26 International Carbohydrate Symposium
    • Place of Presentation
      Madrid (Spain)
    • Year and Date
      2012-07-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of Aglycon Structure on Glycosylation by Cells2012

    • Author(s)
      T. Kimura, K. Miyamura, M. C. Z. Kasuya, K. Hatanaka
    • Organizer
      26 International Carbohydrate Symposium
    • Place of Presentation
      Madrid (Spain)
    • Year and Date
      2012-07-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Incorporation of Alkyl and Fluoroalkyl Glycosides into Cell Membrane and Production of Glycolipid Analogues, Groupe d'Etude et de2011

    • Author(s)
      K. Hatanaka
    • Organizer
      Recherche en Lipidomique
    • Place of Presentation
      Lyon (France)
    • Year and Date
      2011-10-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Incorporation of Fluoro- alkyl Glycosides to Cell Membrane and Saccharide Chain Elongation by Cellular Enzymes2011

    • Author(s)
      K. Hatanaka
    • Organizer
      International Symposium on Fluorous Technologies 2011
    • Place of Presentation
      Hong-Kong (China)
    • Year and Date
      2011-01-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Liposomal Uptake of Fluorescent Fluoroalkyl Glycoside2011

    • Author(s)
      R. Katayama, M. C. Z. Kasuya, K. Hatanaka
    • Organizer
      International Symposium on Fluorous Technologies 2011
    • Place of Presentation
      Hong-Kong (China).
    • Year and Date
      2011-01-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Incorporation of Fluoroalkyl Glycoside to Cell Membrane and Saccharide Chain Elongation by Cellular enzymes2011

    • Author(s)
      K. Hatanaka
    • Organizer
      International Symposium on Fluorous Technologies 2011(招待講演)
    • Place of Presentation
      Hong Kong,China
    • Related Report
      2011 Research-status Report
  • [Presentation] An Efficient Method in Searching for Glycosylation Regulating Compounds

    • Author(s)
      Yukari Nishiyama
    • Organizer
      26 International Carbohydrate Symposium
    • Place of Presentation
      Madrid (Spain)
    • Related Report
      2012 Annual Research Report
  • [Book] Incorporation of Fluorous Glycosides to Cell Membrane and Saccharide Chain Elongation by Cellular Enzymes, Topics in Current Chemistry 308, Special Issue "Fluorous Chemistry"2011

    • Author(s)
      K. Hatanaka
    • Publisher
      edited by Istvan T. Horvath (Published
    • Related Report
      2012 Final Research Report
  • [Book] Melanoma Cell Factory for Glycolipid Production2011

    • Author(s)
      M. C. Z. Kasuya, K. Hatanaka,
    • Publisher
      Breakthroughs in Melanoma Research edited by Yohei Tanaka (Published by InTech)
    • Related Report
      2012 Final Research Report
  • [Book] MELANOMA RESEARCH (Chapter 6, pp103-118)2011

    • Author(s)
      M.C.Z. Kasuya and K. Hatanaka
    • Total Pages
      628
    • Publisher
      INTECH
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.chembio.t.u-tokyo.ac.jp/labs/hatanaka.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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