DEVELOPMENT OF ON/OFF FLUORESCENT PROBES FOR PROTEINS BASED ON RESTRICTION OF INTRAMOLECULAR ROTATION
Project/Area Number |
23651242
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
|
Research Institution | Doshisha University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 蛍光 / 蛍光センシング / BODIPY / 炭酸脱水酵素 / カナマイシン / カナマイシン修飾酵素 / カナマイシン耐性菌 / ^19F-NMR / 回転阻害 / 抗生物質 / 抗生物質修飾酵素 / 酵素 / 酵素阻害剤 / 抗生物質耐性菌 / センシング |
Research Abstract |
Phenyl-BODIPY probes having an enzyme inhibitor or enzyme substrate moiety as a substituent binds to the corresponding enzyme, thereby undergoing restriction of internal rotation of the probe to result in enhanced fluorescence. 19F-NMR indicates that internal rotation in concern is indeed inhibited in the probes subject to complexation-induced fluorescence enhancement. The present tool is applicable to a wide range of proteins (enzymes and receptors) and that bind to a small molecules and is characteristically versatile in that respect.
|
Report
(3 results)
Research Products
(19 results)
-
-
[Presentation] 抗生物質耐性菌の蛍光センシング2012
Author(s)
井出敬一朗・清水康映・山本達望・青山安宏
Organizer
第64回有機合成化学協会関東支部新潟シンポジウム
Place of Presentation
長岡技術科学大学、長岡、新潟
Year and Date
2012-12-01
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-