reconstruction of an external stimulus-dependent replication system

• Project/Area Number: 23655164
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: WATANABE Satoru 独立行政法人理化学研究所, 独立行政法人理化学研究所・生命システム研究センター, 上級研究員 (80300945)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 複製 / 合成生物学 / DNA / 抗体 / 再構成 / cis-特異的 / 無細胞合成系 / 分子進化

Research Abstract

Human has a natural gift to select appropriate antibody molecules from huge variety of molecular library, while an engineering technique that reconstructs the selection process against an antigen of interest in a test tube is immature because the variety of the library molecules is a critical and limiting factor of the current technique. I propose a new and challenging idea that breaks the limitation by looking each DNA molecule as a test tube. The feasibility of the idea was experimentally studied and some elementary steps of the idea were shown to be realized.

Research Products

• [Journal Article] Flexible and rigid structures in HIV-1 p17 matrix protein monitored by relaxation and amide proton exchange with NMR (2014)
  • Author(s): Ohori, Y., H. Okazaki, S. Watanabe, N. Tochio, M. Arai, T. Kigawa and C. Nishimura
  • Journal Title: Biochimica Et Biophysica Acta-Proteins and Proteomics Volume: 1844(3) Pages: 520-526
  • Peer Reviewed
• [Journal Article] Flexible and rigid structures in HIV-1 p17 matrix protein monitored by relaxation and amide proton exchange using NMR (2014)
  • Author(s): Ohori, Y., Okazaki, H., Watanabe, S., Tochio, N., Arai, M., Kigawa, T., and Nishimura, C.*
  • Journal Title: Biochimica et Biophysica Acta Volume: 1844 Issue: 3 Pages: 520-526
  • DOI: 10.1016/j.bbapap.2013.12.010
  • Peer Reviewed
• [Journal Article] Cell-free synthesis system suitable for disulfide-containing proteins (2013)
  • Author(s): Matsuda, T., S. Watanabe and T. Kigawa
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 431(2) Pages: 296-301
  • Peer Reviewed
• [Journal Article] Cell-free synthesis system suitable for disulfide-containing proteins (2013)
  • Author(s): T.Masuda, S.Watanabe, T.Kigawa
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 431 Issue: 2 Pages: 296-301
  • DOI: 10.1016/j.bbrc.2012.12.107
  • Peer Reviewed