| Project/Area Number |
23656532
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Osaka Prefecture University |
|---|
Principal Investigator |
HARA Masayuki 大阪府立大学, 理学(系)研究科(研究院), 教授 (50344172)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MORI Hideki 大阪府立大学, 大学院・理学系研究科, 助教 (30450894)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 神経幹細胞 / 間葉系幹細胞 / 細胞分離技術 / 光増感色素 / PDT / 細胞死 / 活性酸素 / 細胞分化 / 神経幹・前駆細胞 / apoptosis / 神経細胞 / グリア細胞 / ABC transporter / necrosis |
|---|
| Research Abstract |
We cultured neural stem/progenitor cells, mesenchymal stem cells. We measured the uptake of photosensitizer dyes into those cells. Those cells were injured by reactive oxygen species (ROS) evolved under illumination of strong actinic light. Various informations about injury of those cells were obtained, although the method to select immature and differentiated cells was still in development. Further effort is necessary to establish the method, including the establishment of related experimental conditions.
|
