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Development of a novel screening method to isolate microorganisms that produce potential fuel biogas

Research Project

Project/Area Number 23656607
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Energy engineering
Research InstitutionKagoshima University

Principal Investigator

UEDA Takehiko  鹿児島大学, 理工学研究科, 准教授 (80293893)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsマイクロ培養器 / スクリーニング / 燃料ガス / プレーティング / 微生物 / マイクロ流路 / 育種 / コンビナトリアル / 微細パターン / プロファイリング
Research Abstract

Microsphere incubator is a microscale carrier for incubation of a single cell of microorganism. One can isolate microorganisms one by one that can produce potential fuel gas, such as hydrogen, methane and so on, in significantly high efficiency from a huge variety of candidate microorganisms in natural environments, because the produced gas is accumulated inside the microsphere incubator which gives them buoyancy for driving them up to the medium/air interface.
Developing and improving this method leads us to establish a novel fermentation methodology to find out undiscovered microorganisms with highly potent activity to produce fuel gases from wastes or materials of low utility.
In this research, the authors succeeded to prepare specially designed microsphere incubators and to isolate several kinds of candidate microorganisms that produced biogas from natural environment including some of extreme volcanic environments in Kagoshima prefecture.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (13 results)

  • [Journal Article] ISOLATION AND BREEDING OF MICROORGANISMS FOR HIGHER BIOGAS-PRODUCING ACTIVITY BY A NOVEL BIO2012

    • Author(s)
      Takehiko Ueda, Yuta Kodani, Masayo Fukuya, Ryu Imoto, Fukuichi Kaida
    • Journal Title

      Proceedings Venice 2012, Fourth International Symposium on Energy from Biomass and Waste

      Volume: 420 Pages: 1-12

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] マイクロスフィアスクリーニング法による屋久島土壌中のガス産生微生物の高度分布解析2011

    • Author(s)
      榎園千里知, 鳥越祐作, 上田岳彦
    • Journal Title

      鹿児島大学工学部研究報告

      Volume: 53 Pages: 37-41

    • NAID

      40019156848

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Breeding of Microorganisms for Higher Biogasproducing Activity by a Novel Screening Method using Surface Modified Porous Glass Microspheres2011

    • Author(s)
      Takehiko Ueda, Chisato Enokizono, Yuta Kodani, Masayo Fukuya, Ryu Imoto and Fukuichi Kaida
    • Journal Title

      Proceedings of the 4th International Conference on Biotechniques for Air Pollution Control

      Pages: 319-326

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Thermally Responsible Microfluidic Valves Composed of Agarose Hydrogel with Partially Hydrophobized Chitosan2013

    • Author(s)
      Takehiko Ueda, Mio Bansho, and Naoki Mori
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano-Science 2013
    • Place of Presentation
      Kagoshima University, Kagoshima
    • Year and Date
      2013-10-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Thermally Responsible Microfluidic Valves Composed of Agarose Hydrogel with Partially Hydrophobized Chitosan2013

    • Author(s)
      Takehiko Ueda
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano-Science 2013
    • Place of Presentation
      鹿児島県鹿児島市 鹿児島大学稲盛会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] ISOLATION AND BREEDING OF MICROORGANISMS FOR HIGHER BIOGAS-PRODUCING ACTIVITY BY A NOVEL BIOSPHERE SCREENING METHOD2012

    • Author(s)
      Takehiko Ueda, Yuta Kodani, Masayo Fukuya, Ryu Imoto, Fukuichi Kaida
    • Organizer
      4th International Symposium On Energy From Biomass And Waste
    • Place of Presentation
      Rimini, Italy
    • Year and Date
      2012-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 表面機能化した微生物担持マイクロスフィアを利用した環境試料からのガス産生微生物の単離と同定2012

    • Author(s)
      榎園 千里知, 迫田 唯, 上田 岳彦
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      神奈川県
    • Year and Date
      2012-03-22
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 音叉型水晶発振子と複合した高分子ワイヤのレオロジー変化を用いた化学センサ2012

    • Author(s)
      大久保 洋志, 上坂元 志, 上田 岳彦
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      神奈川県
    • Year and Date
      2012-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ISOLATION AND BREEDING OF MICROORGANISMS FOR HIGHER BIOGAS-PRODUCING ACTIVITY BY A NOVEL BIOSPHERE SCREENING METHOD2012

    • Author(s)
      Takehiko Ueda, Yuta Kodani, Masayo Fukuya, Ryu Imoto, Fukuichi Kaida
    • Organizer
      4th International Symposium On Energy From Biomass And Waste
    • Place of Presentation
      イタリア
    • Related Report
      2012 Research-status Report
  • [Presentation] Breeding of Microorganisms for Higher Biogasproducing Activity by a Novel Screening Method using Surface Modified Porous Glass Microspheres2011

    • Author(s)
      Takehiko Ueda
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano-Science
    • Place of Presentation
      National Cheng Kung University, Taiwan
    • Year and Date
      2011-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Gas Sensing through Rheological Responses of Polymer Matrix between Quartz Crystal Tuning Forks2011

    • Author(s)
      Hiroshi Okubo, Shingo Mori, Motonori Akano, Mio Bansho, Takehiko Ueda
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano-Science
    • Place of Presentation
      National Cheng Kung University, Taiwan
    • Year and Date
      2011-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Elevation Distribution of Gas-producing Microorganisms in Yakushima Island. Determined by Microsphere Buoyant Screening Method2011

    • Author(s)
      Chisato Enokizono, Yuta Kotani, Masahiro Furuno, Yusei Mori, Ryu Imoto, Fukuichi Kaida, Takehiko Ueda
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano- Science
    • Place of Presentation
      National Cheng Kung University, Taiwan
    • Year and Date
      2011-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Breeding of Microorganisms for Higher Biogas-producing Activity by a Novel Screening Method using Surface Modified Porous Glass Microspheres2011

    • Author(s)
      Takehiko Ueda, Chisato Enokizono, Yuta Kodani, Masayo Fukuya, Ryu Imoto, Fukuichi Kaida
    • Organizer
      Biotechniques 2011(IVth International Conference on Biotechniques for Air Pollution Control)
    • Place of Presentation
      University of A Coruna, Spain
    • Year and Date
      2011-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Breeding of Microorganisms for Higher Biogas-producing Activity by a Novel Screening Method using Surface Modified Porous Glass Microspheres2011

    • Author(s)
      Takehiko Ueda, Chisato Enokizono, Yuta Kodani, Masayo Fukuya, Ryu Imoto and Fukuichi Kaida
    • Organizer
      Biotechniques 2011(IVth International Conference on Biotechniques for Air Pollution Control)
    • Place of Presentation
      スペイン
    • Related Report
      2011 Research-status Report
  • [Presentation] Elevation Distribution of Gas-producing Microorganisms in Yakushima IslandDetermined by Microsphere Buoyant Screening Method2011

    • Author(s)
      C. Enokizono, Y. Kotani, M. Furuno, Y. Mori, Y. Imoto, F. Kaida, T. Ueda
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano-Science
    • Place of Presentation
      台湾
    • Related Report
      2011 Research-status Report
  • [Presentation] Breeding of Microorganisms for Higher Biogas-producing Activity by a Novel Screening Method using Surface Modified Porous Glass Microspheres2011

    • Author(s)
      Takehiko Ueda
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nano-Science
    • Place of Presentation
      台湾
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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