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Perturbation analysis of the 9+2 pattern of the ciliary/flagellar axoneme

Research Project

Project/Area Number 23657046
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionThe University of Tokyo

Principal Investigator

HIRNO Masafumi  東京大学, 大学院・理学系研究科, 准教授 (10212177)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords微細構造 / 微小管 / タンパク質 / 中心体
Research Abstract

Almost all motile cilia and flagella have a characteristic structure called the “9+2”. However, it has not been rationally explained why the 9+2 pattern is necessary for the ciliary or flagellar motility. The aim of this study is to examine motilities of axonemes with variable numbers of the outer doublet microtubules produced by a Chlamydomonas mutant, bld12. We found several interesting features about interactions between projections in the axonemal structures. These findings are important to understand mechanisms of the assembly and function of the 9+2 structure.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility2013

    • Author(s)
      Yamamoto,R. et al.
    • Journal Title

      J.Cell Biol.

      Volume: 201 Issue: 2 Pages: 263-278

    • DOI

      10.1083/jcb.201211048

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of retrograde intraflagellar transport in flagellar assembly and function.2012

    • Author(s)
      Engel, B. D. et al.
    • Journal Title

      J.Cell Biol.

      Volume: 199 Issue: 1 Pages: 151-167

    • DOI

      10.1083/jcb.201206068

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scaffolding function of theChlamydomonas procentriole protein CRC70, a member of the conserved Cep70 family2011

    • Author(s)
      Shiratsuchi,G.,Kamiya,R.,and Hirono,M.
    • Journal Title

      J. Cell Sci

      Volume: 124 Issue: 17 Pages: 2964-2975

    • DOI

      10.1242/jcs.084715

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structures of SAS-6 suggest its organization in centrioles2011

    • Author(s)
      van Breugel,M.,Hirono,M.,Andreeva,A.,Yanagisawa,H. A.,Yamaguchi,S.,Nakazawa,Y.,Morgner,N.,Petrovich,M.,Ebong,I.O.,Robinson,C.V.,Johnson,C.M.,Veprintsev,D.,Zuber,B.
    • Journal Title

      Science

      Volume: 331 Issue: 6021 Pages: 1196-1199

    • DOI

      10.1126/science.1199325

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Interaction between SAS-6 and Bld10p, cartwheel components that establish the ninefold symmetry of the centriole2012

    • Author(s)
      Noga, A., Yamaguchi, S., Kamiya, R., and Hirono, M.
    • Organizer
      第64回日本細胞生物学会大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Direct interaction between SAS-6 and Bld10p, the two known cartwheel components that establish the ninefold symmetry of the centriole2012

    • Author(s)
      Hirono, M.
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      神戸、神戸国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Novel features in the formation and function of the “9+2” axoneme revealed by a basal-body-deficient mutant of Chlamydomonas2012

    • Author(s)
      Hirono, M.
    • Organizer
      Symposium "Structural biology of flagella/cilia/centriole"
    • Place of Presentation
      Paul Scherrer Institute, Switzerland
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Afm1, a conserved component of the flagellar outer doublet microtubule, is required for generation of the proper asymmetrical waveforms2011

    • Author(s)
      Fujita, A., Yanagisawa, H., Kamiya, R., and Hirono, M.
    • Organizer
      第63回日本細胞生物学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein associated with TTLL9, an enzyme that catalyzes tubulinpolyglutamylation in the axoneme2011

    • Author(s)
      Kubo, T., Yanagisawa, H., Liu, Z., Hirono, M., and Kamiya, R.
    • Organizer
      第63回日本細胞生物学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] Mechanism for establishing the "nine-ness" of the centriole structure" Symposium "Biomimetics focused on the life style of Protista2011

    • Author(s)
      Hirono, M.
    • Organizer
      第49会日本生物物理学会年会
    • Place of Presentation
      姫路
    • Related Report
      2012 Final Research Report
  • [Presentation] A self-organizing mechanism for assembly of the conserved9-fold symmetrical structure of the centriole. Symposium "Challenges for innovation on the basis of protozoan principles2011

    • Author(s)
      Hirono, M.
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Afm1, a conserved component of the flagellar outer doublet microtubule, is required for generation of the proper asymmetrical waveforms2011

    • Author(s)
      Hirono, M., Fujita, A., Yanagisawa, H., Wakabayashi, K., and Kamiya, R.
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/seiri/chlamy/lab.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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