Perturbation analysis of the 9+2 pattern of the ciliary/flagellar axoneme

• Project/Area Number: 23657046
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo
• Principal Investigator: HIRNO Masafumi 東京大学, 大学院・理学系研究科, 准教授 (10212177)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 微細構造 / 微小管 / タンパク質 / 中心体

Research Abstract

Almost all motile cilia and flagella have a characteristic structure called the “9+2”. However, it has not been rationally explained why the 9+2 pattern is necessary for the ciliary or flagellar motility. The aim of this study is to examine motilities of axonemes with variable numbers of the outer doublet microtubules produced by a Chlamydomonas mutant, bld12. We found several interesting features about interactions between projections in the axonemal structures. These findings are important to understand mechanisms of the assembly and function of the 9+2 structure.

Research Products

• [Journal Article] The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility (2013)
  • Author(s): Yamamoto,R. et al.
  • Journal Title: J.Cell Biol. Volume: 201 Issue: 2 Pages: 263-278
  • DOI: 10.1083/jcb.201211048
  • Peer Reviewed
• [Journal Article] The role of retrograde intraflagellar transport in flagellar assembly and function. (2012)
  • Author(s): Engel, B. D. et al.
  • Journal Title: J.Cell Biol. Volume: 199 Issue: 1 Pages: 151-167
  • DOI: 10.1083/jcb.201206068
  • Peer Reviewed
• [Journal Article] Scaffolding function of theChlamydomonas procentriole protein CRC70, a member of the conserved Cep70 family (2011)
  • Author(s): Shiratsuchi,G.,Kamiya,R.,and Hirono,M.
  • Journal Title: J. Cell Sci Volume: 124 Issue: 17 Pages: 2964-2975
  • DOI: 10.1242/jcs.084715
  • Peer Reviewed
• [Journal Article] Structures of SAS-6 suggest its organization in centrioles (2011)
  • Author(s): van Breugel,M.,Hirono,M.,Andreeva,A.,Yanagisawa,H. A.,Yamaguchi,S.,Nakazawa,Y.,Morgner,N.,Petrovich,M.,Ebong,I.O.,Robinson,C.V.,Johnson,C.M.,Veprintsev,D.,Zuber,B.
  • Journal Title: Science Volume: 331 Issue: 6021 Pages: 1196-1199
  • DOI: 10.1126/science.1199325
  • Peer Reviewed
• [Presentation] Interaction between SAS-6 and Bld10p, cartwheel components that establish the ninefold symmetry of the centriole (2012)
  • Author(s): Noga, A., Yamaguchi, S., Kamiya, R., and Hirono, M.
  • Organizer: 第64回日本細胞生物学会大会
  • Place of Presentation: 神戸
• [Presentation] Direct interaction between SAS-6 and Bld10p, the two known cartwheel components that establish the ninefold symmetry of the centriole (2012)
  • Author(s): Hirono, M.
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 神戸、神戸国際会議場
• [Presentation] Novel features in the formation and function of the “9+2” axoneme revealed by a basal-body-deficient mutant of Chlamydomonas (2012)
  • Author(s): Hirono, M.
  • Organizer: Symposium "Structural biology of flagella/cilia/centriole"
  • Place of Presentation: Paul Scherrer Institute, Switzerland
  • Invited
• [Presentation] Afm1, a conserved component of the flagellar outer doublet microtubule, is required for generation of the proper asymmetrical waveforms (2011)
  • Author(s): Fujita, A., Yanagisawa, H., Kamiya, R., and Hirono, M.
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 札幌
• [Presentation] A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein associated with TTLL9, an enzyme that catalyzes tubulinpolyglutamylation in the axoneme (2011)
  • Author(s): Kubo, T., Yanagisawa, H., Liu, Z., Hirono, M., and Kamiya, R.
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 札幌
• [Presentation] Mechanism for establishing the "nine-ness" of the centriole structure" Symposium "Biomimetics focused on the life style of Protista (2011)
  • Author(s): Hirono, M.
  • Organizer: 第49会日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] A self-organizing mechanism for assembly of the conserved9-fold symmetrical structure of the centriole. Symposium "Challenges for innovation on the basis of protozoan principles (2011)
  • Author(s): Hirono, M.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Afm1, a conserved component of the flagellar outer doublet microtubule, is required for generation of the proper asymmetrical waveforms (2011)
  • Author(s): Hirono, M., Fujita, A., Yanagisawa, H., Wakabayashi, K., and Kamiya, R.
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学（北海道）
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seiri/chlamy/lab.html