Development of Light-induced Membrane Proteins as a High-sensitiveMembrane Potential Sensor
Project/Area Number |
23657096
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | Hokkaido University |
Principal Investigator |
DEMURA Makoto 北海道大学, 大学院・先端生命科学研究院, 教授 (70188704)
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Project Period (FY) |
2012 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | ハロロドプシン / オプトジェネティクス / 光遺伝学 / 膜タンパク質 / 光生物学 / 神経回路 / バイオコンピューター / ロドプシン / 神経 / 光吸収 |
Research Abstract |
Human eye has a light-sensitive membrane protein. This sensor protein called rhodopsin that can identify the color of the light. Contrary, a rhodopsin-like proteins were found in some microorganisms, which have a light-driven ion transport (membrane potential) features different from the eyes. In this study, in order to improve the light-driven ion transport of microbial rhodopsin, the modified rhodopsins were prepared and visible absorption spectra in equilibrium and transient states, and the trimeric structure of modified rhodopsins were investigated.
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Report
(3 results)
Research Products
(74 results)
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[Journal Article] Salt bridge in the conserved His-Asp cluster in Gloeobacter rhodopsin contributes to trimer formation2013
Author(s)
Tsukamoto, T., Kikukawa, T., Kurata, T., Jung, K-H, Kamo, N., Demura, M.
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Journal Title
FEBS Lett.
Volume: 587
Issue: 4
Pages: 322-327
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Homo-trimer Formation and Dissociation of pharaonis Halorhodopsin in Detergent System2012
Author(s)
Tsukamoto, T., Sasaki, T., Fujimoto, K. J., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Kamo, N., Demura, M
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Journal Title
Biophys. J
Volume: 102
Issue: 12
Pages: 2906-2915
DOI
Related Report
Peer Reviewed
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[Journal Article] Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation2012
Author(s)
Furutani, Y., Fujiwara, K., Kimura, T., Kikukawa, T., Demura, M., Kandori, H
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Journal Title
J. Phys. Chem. Lett
Volume: 3
Issue: 20
Pages: 2964-2969
DOI
Related Report
Peer Reviewed
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[Presentation] Homo-trimerパク質ハロロドプシンの研究2012
Author(s)
T. Tsukamoto, T. Sasaki, K. J. Fujimoto, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
Organizer
日本蛋白質科学会年会
Place of Presentation
名古屋国際会議場(名古屋市)
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[Presentation] The effect of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I.2012
Author(s)
Reissig, L., Iwata, T., Kikukawa, T., Demura, M., Kamo, N., Kandori, H., and Sudo, Y.
Organizer
15th Int Conf. Retinal Proteins
Place of Presentation
Ascona, Switzerland
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[Presentation] Expression, purification and structure analysis of ABF-2(Anti Bacterial Factor-2), an antimicrobial peptide derived from C.elegans2011
Author(s)
Kanako Ito, Tomoyasu Aizawa, Masakatsu Kamiya, Yoshitaka Umetsu, Yusuke Kato, Yasuhiro Kumaki, Takashi Kikukawa, Makoto Demura, Keiichi Kawano
Organizer
日本生物物理学会年会
Place of Presentation
兵庫県立大学姫路書写キャンパス(兵庫)
Related Report
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