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Establishment of the cell-type-specific RNAi method for the analysis of cellular dynamics during animal development

Research Project

Project/Area Number 23657139
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

KUBOTA YUKIHIKO  東北大学, 生命科学研究科, 助教 (70333325)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsPAF1複合体 / 表皮形態形成 / 胚発生 / 線虫・C. elegans / 細胞ポジショニング / 細胞移動 / 細胞変形 / C. elegans / 形態形成 / 細胞動態 / RNAi / 遺伝子ノックダウン / 表皮細胞 / 組織間相互作用 / 細胞骨格
Research Abstract

We found that, in the nematode C. elegans, the PAF1C is involved in epidermal morphogenesis in late embryogenesis. In RNAi knockdown embryos of the components of the PAF1C (CTR-9, PAFO-1, LEO-1, CDC-73, RTFO-1), whereas the number and cell fate determination of epidermal cells were apparently unaffected, their position and shape were severely disorganized. PAFO-1::mCherry, mCherry::LEO-1 and GFP::RTFO-1 driven by the authentic promoters were detected in the nuclei of a wide range of cells. Epidermis-specific expression of mCherry::LEO-1 rescued embryonic lethality of the leo-1 deletion mutant. Thus, although the PAF1C is universally expressed in C. elegans embryos, its epidermal function is crucial for the viability of this animal.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] The PAF1 complex is involved in embryonic epidermal morphogenesis in Caenorhabditis elegans.2014

    • Author(s)
      Kubota Y., Tsuyama K., Takabayashi Y., Haruta N., Iida N., Maruyama R., Sugimoto A.
    • Journal Title

      Developmental Biology

      Volume: in press Issue: 1 Pages: 43-53

    • DOI

      10.1016/j.ydbio.2014.04.002

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue Architecture in the C. elegans Gonad Depends on Interactions among Fibulin-1, Type IV collagen and the ADAMTS Extracellular Protease2012

    • Author(s)
      Yukihiko Kubota, Kayo Nagata,Asako Sugimoto and Kiyoji Nishiwaki
    • Journal Title

      Genetics

      Volume: 190

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 線虫C. elegans 胚の表皮形態形成に関与するPAF1 複合体の発現パターンおよび核内局在制御解析2013

    • Author(s)
      津山 研二, 久保田 幸彦, 高林 佑輔, 丸山理香, 飯田 直子, 杉本 亜砂子
    • Organizer
      日本分子生物学会第35回年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] The PAF1 complex is essential for epidermal morphogenesis in C. elegans embryos2013

    • Author(s)
      Yukihiko Kubota, Yusuke Takabayashi, Kenji Tsuyama, Nami Haruta, Rika Maruyama , Asako Sugimoto
    • Organizer
      19th international worm meeting
    • Place of Presentation
      アメリカ・ロスアンゼルス・UCLA
    • Year and Date
      2013-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 線虫C. elegans の表皮形態形成にPAF1 複合体は必須の役割を果たす2012

    • Author(s)
      久保田 幸彦, 高林 佑輔, 丸山 理香, 杉本 亜砂子
    • Organizer
      日本分子生物学会第35回年会
    • Place of Presentation
      福岡国際会議場 マリンメッセ福岡(福岡県福岡市)
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 線虫C. elegans の表皮形態形成にPAF1 複合体は必須の役割を果たす2012

    • Author(s)
      久保田 幸彦, 高林 佑輔, 丸山 理香, 杉本 亜砂子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学(大阪府大阪市)
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] The PAF1 complex is essential for epidermal morphogenesis in C. elegans embryos2012

    • Author(s)
      Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama, Asako Sugimoto
    • Organizer
      EMBO meeting : Morphogenesis and Dynamics of Multicellular Systems
    • Place of Presentation
      ドイツ・ハイデルベルク
    • Year and Date
      2012-09-08
    • Related Report
      2013 Final Research Report
  • [Presentation] The PAF1 complex is essential for epithelial morphogenesis in C. elegans embryos2012

    • Author(s)
      Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama, Asako Sugimoto
    • Organizer
      5th East Asia C. elegans Meeting
    • Place of Presentation
      台湾・台北
    • Year and Date
      2012-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] RNAi screening ofor genes involved in epithelial morphogenesis in C. elegans2011

    • Author(s)
      高林 佑輔, 久保田 幸彦, 丸山 理香, 杉本 亜砂子
    • Organizer
      日本分子生物学会第34回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 線虫C. elegans胚の表皮形態形成に関与するPAF1複合体の発現パターンおよび核内局在制御解析

    • Author(s)
      津山 研二, 久保田 幸彦, 高林 佑輔, 丸山 理香, 飯田 直子, 杉本 亜砂子
    • Organizer
      日本分子生物学会第36回大会
    • Place of Presentation
      神戸ポートアイランド( 兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] The PAF1 complex is essential for epidermal morphogenesis in C. elegans embryos

    • Author(s)
      Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama, Asako Sugimoto
    • Organizer
      EMBO Conference Series “Morphogenesis”
    • Place of Presentation
      Heidelberg, Germany
    • Related Report
      2012 Research-status Report
  • [Presentation] The PAF1 complex is essential for epidermal morphogenesis in C. elegans embryos

    • Author(s)
      久保田 幸彦、高林 佑輔、丸山 理香、杉本 亜砂子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] The PAF1 complex is required for epidermal morphogenesis in C. elegans embryos

    • Author(s)
      Yukihiko Kubota, Yusuke Takabayashi, Kenji Tsuyama, Rika Maruyama, Asako Sugimoto
    • Organizer
      日本分子生物学会第35回大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] An efficient RNAi screening approach for C. elegans embryonic morphogenesis

    • Author(s)
      Yusuke Takabayashi, Yukihiko Kubota, Rika Maruyama and Asako Sugimoto
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www2.lifesci.tohoku.ac.jp/lab-www/sugimoto_lab/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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