System evolution in chorate morphogenesis in view of gene evolution of noncoding exons

• Project/Area Number: 23657159
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Kitasato University
• Principal Investigator: ITO Michihiko 北里大学, 理学部, 准教授 (90240994)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脊椎動物 / 選択的プロモーター / エクソン / 遺伝子進化 / 退化 / 精巣形成 / 生殖細胞 / プロモーター / DMRT1 / イントロン / 非コードエクソン / オルソログ / 分子進化

Research Abstract

To understand how emergence and degeneration of noncoding exon 1 is involved in morphological evolution in chordates, a testis formation-related gene Dmrt1was analyzed using several chordate species. The results indicated that Dmrt1’s noncoding exon 1 might have emerged during vertebrate evolution, which could give the gene two types of promoters, contributing to divergence of expression patterns. However, it might have degenerated because of changes of DMRT1 functions intestis formation and maintenance during amniote evolution.

Research Products

• [Journal Article] 両生類・爬虫類・鳥類の性決定システムおよびその分子機構 (2013)
  • Author(s): 伊藤 道彦
  • Journal Title: 細胞工学 Volume: 32巻 Pages: 181-187
  • URL: http://gakken-mesh.jp/journal/detail/9784780901399.html
• [Journal Article] 両生類・爬虫類・鳥類の性決定システムおよびその分子機構 (2013)
  • Author(s): 伊藤 道彦
  • Journal Title: 細胞工学 Volume: 32 Pages: 181-187
• [Journal Article] Apoptotic and survival signaling mediated through dearth receptor members during metamorphosis in the African clawed frog Xenopus laevis (2012)
  • Author(s): Michihiko Ito
  • Journal Title: General Comparative Endocrinology Volume: 176 Issue: 3 Pages: 461-464
  • DOI: 10.1016/j.ygcen.2011.12.037
  • Peer Reviewed
• [Journal Article] Molecular evolution of vertebrate sex-determining genes (2012)
  • Author(s): Shuuji Mawaribuchi
  • Journal Title: Chromosome Research Volume: 20 Issue: 1 Pages: 139-151
  • DOI: 10.1007/s10577-011-9265-9
  • Peer Reviewed
• [Journal Article] Serine-threonine kinase 38 is regulated by glycogen synthase kinase-3 and modulates oxidative stress-induced cell death (2012)
  • Author(s): Atsushi Enomoto
  • Journal Title: Free Radical Biology and Medicine Volume: 52 Pages: 507-515
  • Peer Reviewed
• [Presentation] Male sex-determining systems during vertebrate evolution? (2012)
  • Author(s): Ito,M
  • Organizer: Turnovers in sex-determination mechanisms
  • Place of Presentation: La Sage, Switzerland
• [Presentation] Molecular evolution of veertebrate Dmrt1 orthlogues (2012)
  • Author(s): Ito,M., et al
  • Organizer: Sixth International Symposium on Vertebrate Sex Determination
  • Place of Presentation: Kona,USA
• [Presentation] Opposite roles of DMRT1 and tsW-linked paralogue (2011)
  • Author(s): Ito,M., et al
  • Organizer: 北米比較内分泌学会
  • Place of Presentation: Ann Arbor,USA
• [Presentation] Opposite roles of DMRT1 and its W-linked paralogue, DM-W, in the ZZ/ZW-type sex-determination in Xenopus leavis (2011)
  • Author(s): Michihiko Ito
  • Organizer: North American Society for Comparative Endocrinology(招待講演)
  • Place of Presentation: Ann Arbor (USA)
• [Presentation] Molecular evolution of vertebrate Dmrt1 homologues
  • Author(s): Michihiko Ito
  • Organizer: Sixth International Symposium on Vertebrate Sex determination
  • Place of Presentation: Kona (Hawaii, USA)
• [Presentation] Male sex-determining systems during vertebrate evolution: did a significant change from the DMRT1-driven system to SRY/SOX9-driven system occur during mammalian evolution?
  • Author(s): Michihiko Ito
  • Organizer: Workshop “Turnovers in sex-determination Mechanisms”
  • Place of Presentation: La Sage (Switzerland)
• [Presentation] Sex determination and differentiation in Xenopus laevis
  • Author(s): Michihiko Ito
  • Organizer: Workshop on Sex determination in Dragons, Frogs, and Fugu fish
  • Place of Presentation: Higashihiroshima
  • Invited
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/sci/resea/seibutsu/joho/index.htm
• [Remarks] 北里大学理学部生物情報科学講座
  • URL: http://www.kitasato-u.ac.jp/sci/resea/seibutsu/joho/studies01.htm