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Development of pesticide for phytoplasma disease targeting a virulence factor

Research Project

Project/Area Number 23658039
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant pathology
Research InstitutionThe University of Tokyo

Principal Investigator

NAMBA Shigetou  東京大学, 大学院・農学生命科学研究科, 教授 (50189221)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords病害防除 / ファイトプラズマ / 病原性因子
Research Abstract

We investigated a phytoplasma population in a plant host treated with an inhibitor of a mechanosensitive channel MscL. As a result, compared with the phytoplasma-infected plant supplied with inhibitor-free water, the growth of phytoplasma was partially suppressed by the inhibitor treatment at three weeks after phytoplasma infection. This suggests that the inhibition of MscL channel may contribute to the development of novel pest controls.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (39 results)

All 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (28 results) Remarks (1 results)

  • [Journal Article] Functional characterization and gene expression profiling of superoxidedismutase from plant pathogenic phytoplasma2012

    • Author(s)
      Miura C. , Sugawara K., Neriya Y., Minato N. , Keima T. , Himeno M., Maejima K., Komatsu K. , Yamaji Y., Oshima K. , Namba S.
    • Journal Title

      Gene

      Volume: 510 Issue: 2 Pages: 107-112

    • DOI

      10.1016/j.gene.2012.09.001

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and reliable detection of phytoplasma by loop-mediated isothermal amplification targeting a housekeeping gene.2012

    • Author(s)
      Sugawara K. , Himeno M., Keima T., Kitazawa Y. , Maejima K., Oshima K Namba S
    • Journal Title

      Journal of General Plant Pathology

      Volume: 78 Issue: 6 Pages: 389-397

    • DOI

      10.1007/s10327-012-0403-9

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning, expression analysis and sequence diversity of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI)2011

    • Author(s)
      Neriya Y., Sugawara K., Maejima K., Hashimoto M., Komatsu K., Minato N., Miura C., Kakizawa S., Yamaji Y., Oshima K., Namba S.
    • Journal Title

      FEMS Microbiology Letters

      Volume: 324 Issue: 1 Pages: 38-47

    • DOI

      10.1111/j.1574-6968.2011.02384.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unique morphological changes in plant pathogenic phytoplasma-infected petunia flowers are related to transcriptional regulation of floral homeotic genes in an organ-specific manner2011

    • Author(s)
      Misako Himeno, et al
    • Journal Title

      Plant Journal

      Volume: 67 Issue: 6 Pages: 971-979

    • DOI

      10.1111/j.1365-313x.2011.04650.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect2011

    • Author(s)
      大島研郎
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e23242-e23242

    • DOI

      10.1371/journal.pone.0023242

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The groEL gene as an additional marker for finer differentiation of'Candidatus Phytoplasma asteris'-related strains2011

    • Author(s)
      Mitrovic,J.,Kakizawa,S.,Duduk,B.,Oshima,K., Namba,S.,and Bertaccini,A.
    • Journal Title

      Ann. Appl. Biol.

      Volume: 159 Issue: 1 Pages: 41-48

    • DOI

      10.1111/j.1744-7348.2011.00472.x

    • Related Report
      2012 Final Research Report
  • [Journal Article] Cloning, expression analysis, and sequence diversity of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI).2011

    • Author(s)
      Neriya, Y., Sugawara, K., Maejima, K., Hashimoto, M., Komatsu, K., Minato, N., Miura, C., Kakizawa, S., Yamaji, Y., Oshima, K., and Namba, S.
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 324 Pages: 38-47

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Unique morphological changes in plant pathogenic phytoplasma-infected petunia flowers are related to transcriptional regulation of floral homeotic genes in an organ-specific manner.2011

    • Author(s)
      Himeno, M., Neriya, Y., Minato, N., Miura, C., Sugawara, K., Ishii, Y., Yamaji, Y., Kakizawa, S., Oshima, K., and Namba, S.
    • Journal Title

      Plant J.

      Volume: 67 Pages: 971-979

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect.2011

    • Author(s)
      Oshima, K., Ishii, Y., Kakizawa, S., Sugawara, K., Neriya, Y., Himeno, M., Minato, N., Miura, C., Shiraishi, T., Yamaji, Y., and Namba, S.
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The groEL gene as an additional marker for finer differentiation of 'Candidatus Phytoplasma asteris'-related strains.2011

    • Author(s)
      Mitrovic, J., Kakizawa, S., Duduk, B., Oshima, K., Namba, S., and Bertaccini, A.
    • Journal Title

      Ann. Appl. Biol.

      Volume: 159 Pages: 41-48

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 葉化症状を示すアジサイより検出されたCandidatus Phytopl asmaasteris HP系統の分子遺伝学的解析2013

    • Author(s)
      桂馬拓也,高橋厚,滝波祐輔,岡野夕香里,前島健作,大島研郎,難波成任
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Final Research Report
  • [Presentation] 葉化症状を示すアジサイより検出されたCandidatus Phytoplasma asteris HP系統の分子遺伝学的解析2013

    • Author(s)
      桂馬拓也・高橋 厚・滝波祐輔・岡野夕香里・前島健作・大島研郎・難波成任
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 葉化症状を引き起こすファイトプラズマのエフェクターについて2012

    • Author(s)
      前島健作,岩井涼,藤田尚子,姫野未紗子,山次康幸,大島研郎,難波成任
    • Organizer
      平成24年度日本植物病理学会関東部会
    • Place of Presentation
      法政大学市ヶ谷キャンパス
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物病原細菌ファイトプラズマのペプチド性エフェクターTENGUの機能解析2012

    • Author(s)
      菅原杏子,湊菜未,小松健,大島研郎,難波成任
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Molecular biological studies on phytoplasmal pathogenicity2012

    • Author(s)
      Namba S.
    • Organizer
      XV International congress of molecular plant-microbe interactions
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] ファイトプラズマのポストゲノム生物学-宿主スイッチングにおける網羅的遺伝子発現解析-2012

    • Author(s)
      大島研郎,三浦千裕,湊菜未,煉谷裕太朗,石川一也,北沢優悟,二條貴通,渡邊翼,前島健作,難波成任
    • Organizer
      日本マイコプラズマ学会第39回学術集会
    • Place of Presentation
      アイーナいわて県民情報交流センター
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマ感染が花の形態形成遺伝子の発現へ与える影響2012

    • Author(s)
      姫野未紗子,湊菜未,煉谷裕太朗,三浦千裕,石川一也,桂馬拓也,前島健作,大島研郎,難波成任
    • Organizer
      日本マイコプラズマ学会第39回学術集会
    • Place of Presentation
      アイーナいわて県民情報交流センター
    • Related Report
      2012 Final Research Report
  • [Presentation] poinsettia branch-inducing phytoplasma(PoiBI)の主要抗原膜タン パク質の発現解析2012

    • Author(s)
      煉谷裕太朗, 岩井涼, 滝波祐輔, 桂馬拓也, 前島健作, 大島研郎, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマ感染ペチュニアにおける花芽分裂組織決定遺伝子の発現変動2012

    • Author(s)
      岩井涼,姫野未紗子,菅原杏子,煉谷裕太朗,小松健,大島研郎,難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマ感染が花のホメオティック遺伝子の発現に与える影響2012

    • Author(s)
      姫野未紗子,菅原杏子,岩井涼,煉谷裕太朗,小松健,大島研郎,難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマの網羅的遺伝子発現解析系の確立2012

    • Author(s)
      大島研郎,三浦千裕,湊菜未,北沢優悟,姫野未紗子,前島健作,難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマの植物-昆虫ホストスイッチングに伴う網羅的発現変動解析2012

    • Author(s)
      三浦千裕,湊菜未,北沢優悟,前島健作,大島研郎,難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマ防除における機械刺激受容チャネル阻害の有効性2012

    • Author(s)
      湊菜未,三浦千裕,桂馬拓也,北沢優悟,前島健作,大島研郎,難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ファイトプラズマのポストゲノム生物学 - 宿主スイッチングにおける網羅的遺伝子解析 -2012

    • Author(s)
      大島研郎・三浦千裕・湊菜未・煉谷裕太朗・石川一也・北沢優悟・二條貴通・渡邊翼・前島健作・難波成任
    • Organizer
      日本マイコプラズマ学会第39回学術集会
    • Place of Presentation
      いわて県民情報交流センター
    • Related Report
      2012 Annual Research Report
  • [Presentation] ファイトプラズマ感染が花の形態形成遺伝子の発現へ与える影響2012

    • Author(s)
      姫野未紗子・湊菜未・煉谷裕太朗・三浦千裕・石川一也・桂馬拓也・前島健作・大島研郎・難波成任
    • Organizer
      日本マイコプラズマ学会第39回学術集会
    • Place of Presentation
      いわて県民情報交流センター
    • Related Report
      2012 Annual Research Report
  • [Presentation] 葉化症状を引き起こすファイトプラズマのエフェクターについて2012

    • Author(s)
      前島健作・岩井涼・藤田尚子・姫野未紗子・山次康幸・大島研郎・難波成任
    • Organizer
      平成24年度日本植物病理学会関東部会
    • Place of Presentation
      法政大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] poinsettia branch-inducing phytoplasma(PoiBI)の主要抗原膜タンパク質の発現解析2012

    • Author(s)
      煉谷裕太朗, 岩井涼, 滝波祐輔, 桂馬拓也, 前島健作, 大島研郎, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡県
    • Related Report
      2011 Research-status Report
  • [Presentation] ファイトプラズマ感染ペチュニアにおける花芽分裂組織決定遺伝子の発現変動2012

    • Author(s)
      岩井涼, 姫野未紗子, 菅原杏子, 煉谷裕太朗, 小松健, 大島研郎, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡県
    • Related Report
      2011 Research-status Report
  • [Presentation] ファイトプラズマ感染が花のホメオティック遺伝子の発現に与える影響2012

    • Author(s)
      姫野未紗子, 菅原杏子, 岩井涼, 煉谷裕太朗, 小松健, 大島研郎, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡県
    • Related Report
      2011 Research-status Report
  • [Presentation] ファイトプラズマの網羅的遺伝子発現解析系の確立2012

    • Author(s)
      大島研郎, 三浦千裕, 湊菜未, 北沢優悟, 姫野未紗子, 前島健作, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡県
    • Related Report
      2011 Research-status Report
  • [Presentation] ファイトプラズマの植物―昆虫ホストスイッチングに伴う網羅的発現変動解析2012

    • Author(s)
      三浦千裕, 湊菜未, 北沢優悟, 前島健作, 大島研郎, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡県
    • Related Report
      2011 Research-status Report
  • [Presentation] ファイトプラズマ防除における機械刺激受容チャネル阻害の有効性2012

    • Author(s)
      湊菜未, 三浦千裕, 桂馬拓也, 北沢優悟, 前島健作, 大島研郎, 難波成任
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡県
    • Related Report
      2011 Research-status Report
  • [Presentation] New Developments in Molecular Biological Research on Plant-Pathogenic Mollicutes: A Tiny Phytoplasma with a Reduced Genome tricks a Giant Plant Industry2011

    • Author(s)
      Namba S.
    • Organizer
      5th Meeting of the Asian Organization for Mycoplasmology
    • Place of Presentation
      Nagasaki ken Medical
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular biological studies on phytoplasmal pathogenicity2011

    • Author(s)
      Oshima, K., Minato, N., Miura, C., Neriya, Y., Shiraishi, T., Sugawara, K., Himeno, M., Kakizawa, S., Namba, S.
    • Organizer
      5th Meeting of the Asian Organization for Mycoplasmology
    • Place of Presentation
      Nagasaki ken Medical Association, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular mechanisms underlying the pathogenicity of phytoplasma2011

    • Author(s)
      Oshima, K., Sugawara, K., Himeno, M., Adachi, T., Ishikawa, K., Takinami, Y., Namba, S.
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] New Developments in Molecular Biological Research on Plant-Pathogenic Mollicutes: A Tiny Phytoplasma with a Reduced Genome tricks a Giant Plant Industry.2011

    • Author(s)
      Namba S.
    • Organizer
      Joint Congress of The 5th Meeting of the Asian Organization for Mycoplasmology (AOM) and The 38th Meeting of the Japanese Society of Mycoplasmology (JSM)(招待講演)
    • Place of Presentation
      長崎県
    • Related Report
      2011 Research-status Report
  • [Presentation] 葉化症状を示すアジサイより初めて検出されたファイトプラズマ種とその検出キットの開発について2011

    • Author(s)
      滝波祐輔・高橋 厚・石川一也・足立達司・三浦千裕・煉谷裕太朗・前島健作・難波成任
    • Organizer
      平成23年度日本植物病理学会関東部会
    • Place of Presentation
      茨城県
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular mechamisms underlying the pathogenicity of phytoplasma.2011

    • Author(s)
      Oshima K., Sugawara K., Himeno M., Adachi T., Ishikawa K., Takinami Y., Namba S.
    • Organizer
      XIII, International Congress of Bacteriology and Applied Microbiology(招待講演)
    • Place of Presentation
      北海道
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://papilio.ab.a.u-tokyo.ac.jp/planpath/index.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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