| Project/Area Number |
23658085
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
KOYAMA Sumihiro 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (50344297)
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| Project Period (FY) |
2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 環境微生物 / 微生物の電気誘引付着 / 微生物の電気剥離回収 / 大腸菌 / 枯草菌 / Bacillus halodurans / Shewanella violacea / Kocuria rosea / Shewanella oneidensis |
|---|
| Research Abstract |
The author developed an electrical modulation method for attachment and detachment of microorganisms. When we applied this method to separate microorganisms from deep-sea sediment, bacteria belonging to 19 phyla and 23 classes were collected without undesirable high molecular-weight contaminants such humic acids. At the phylum level 95% and at the class level 87% of the phylotypes among electrically retrieved bacteria were common to the gene clones from the direct sediment DNA extraction.
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