Screening of posttranslationally modified peptide containing isoprenylated tryptophane residue.

• Project/Area Number: 23658098
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Chubu University
• Principal Investigator: OKADA Masahiro 中部大学, 応用生物学部, 講師 (40377792)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 翻訳後修飾 / イソプレニル化 / トリプトファン / 枯草菌 / クオラムセンシング

Research Abstract

In order to confirm that isoprenylation of tryptophan residues is a universal posttranslational modification, I developed a cell-free assay system by which the tryptophan residue in the ComX pheromone precursor can be isoprenylated by themodifying enzyme, ComQ, with isoprenyl pyrophosphate and magnesium ion.

Research Products

• [Journal Article] Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor (2012)
  • Author(s): F. Tsuji, A. Ishihara, A. Nakagawa, M. Okada, S. Kitamura, K. Kanamaru, Y. Masuda, K. Murakami, K. Irie, and Y. Sakagami.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 8 Pages: 1492-1496
  • DOI: 10.1271/bbb.120206
  • NAID: 10031065914
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Creation of pure nanodrugs and their anticancer properties (2012)
  • Author(s): H. Kasai, T. Murakami, Y. Ikuta, Y. Koseki, K. Baba, H. Oikawa, H. Nakanishi, M. Okada, M. Shoji, M. Ueda, H. Imahori, M. Hashida
  • Journal Title: Angew. Chem. Int. Ed Volume: 51 Issue: 41 Pages: 10315-10318
  • DOI: 10.1002/anie.201204596
  • Peer Reviewed
• [Journal Article] Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system (2012)
  • Author(s): Tsuji, F., Ishihara, A., Kurata, K., Nakagawa, A., Okada, M., Kitamura, S., Kanamaru, K., Masuda, Y., Murakami, K., Irie, K., Sakagami, Y.
  • Journal Title: FEBS Letters Volume: 586 Issue: 2 Pages: 174-179
  • DOI: 10.1016/j.febslet.2011.12.012
  • Peer Reviewed
• [Journal Article] Post-Translational Isoprenylation of Tryptophan (2011)
  • Author(s): M. Okada.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 8 Pages: 1413-1417
  • DOI: 10.1271/bbb.110087
  • NAID: 10029590897
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] Peptide isoprenylation (2012)
  • Author(s): M. Okada.
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2012 (招待講演)
  • Place of Presentation: Hangzhou, China
• [Presentation] Peptide isoprenylation (2012)
  • Author(s): M. Okada.
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2012
  • Place of Presentation: Hangzhou, China
  • Invited
• [Presentation] 翻訳後修飾によるトリプト ファンのイソプレニル化 (2011)
  • Author(s): 岡田 正弘.
  • Organizer: 第三回有機「ものつくり」化学研究会
  • Place of Presentation: 福岡(招待講演)
  • Year and Date: 2011-08-27
• [Presentation] 翻訳後修飾によるトリプトファンのイソプレニル化 (2011)
  • Author(s): 岡田 正弘
  • Organizer: 第三回 有機「ものつくり」化学研究会(招待講演)
  • Place of Presentation: 福岡
• [Book] Posttranslational isoprenylation of tryptophan residues in Bacillus subtilis. The Enzymes (2011)
  • Author(s): M. Okada, F. Tsuji and Y.Sakagami.
  • Publisher: Academic Press, San Diego, CA.
• [Book] The Enzymes Vol 29 (2011)
  • Author(s): M. Okada, F. Tsuji and Y. Sakagami.
  • Publisher: Academic Press