Screening of posttranslationally modified peptide containing isoprenylated tryptophane residue.
Project/Area Number |
23658098
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bioproduction chemistry/Bioorganic chemistry
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Research Institution | Chubu University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 翻訳後修飾 / イソプレニル化 / トリプトファン / 枯草菌 / クオラムセンシング |
Research Abstract |
In order to confirm that isoprenylation of tryptophan residues is a universal posttranslational modification, I developed a cell-free assay system by which the tryptophan residue in the ComX pheromone precursor can be isoprenylated by themodifying enzyme, ComQ, with isoprenyl pyrophosphate and magnesium ion.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor2012
Author(s)
F. Tsuji, A. Ishihara, A. Nakagawa, M. Okada, S. Kitamura, K. Kanamaru, Y. Masuda, K. Murakami, K. Irie, and Y. Sakagami.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Issue: 8
Pages: 1492-1496
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Creation of pure nanodrugs and their anticancer properties2012
Author(s)
H. Kasai, T. Murakami, Y. Ikuta, Y. Koseki, K. Baba, H. Oikawa, H. Nakanishi, M. Okada, M. Shoji, M. Ueda, H. Imahori, M. Hashida
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Journal Title
Angew. Chem. Int. Ed
Volume: 51
Issue: 41
Pages: 10315-10318
DOI
Related Report
Peer Reviewed
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[Journal Article] Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system2012
Author(s)
Tsuji, F., Ishihara, A., Kurata, K., Nakagawa, A., Okada, M., Kitamura, S., Kanamaru, K., Masuda, Y., Murakami, K., Irie, K., Sakagami, Y.
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Journal Title
FEBS Letters
Volume: 586
Issue: 2
Pages: 174-179
DOI
Related Report
Peer Reviewed
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