Molecular, cellular and phylogenetic aspects of selective inhibition of early periods in metazoan ontogenesis by sponge and other substances
| Project/Area Number |
23658102
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Keio University |
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Principal Investigator |
IKEGAMI Susumu 慶應義塾大学, 自然科学研究教育センター, 訪問教授 (80011980)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Hiroyuki 慶應義塾大学, 文学部, 教授 (20169577)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ヒトデ発生 / 発生阻害 / Ancorinoside A / Exiguamide / 若狭湾 |
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| Research Abstract |
Following fertilization, the starfish, Asterina pectinifera, embryo develops to the blastula and then undergoes gastrulation to form a gut and create a feeding bipinnaria larva. The larva then takes on a more elaborate form called a brachioraria larva. Several substances obtained from sponges specifically prevent starfish embryonic development at the blastula or the gastrula stages. Screening search for synthetic compounds which mimic the action of these substances was undertaken but such compounds were not detected.
The study was then focused on the effect of loss of nuclear transglutaminase, a protein that is phylogenetically unique in the starfish embryo, by microinjecting the morpholino antisense oligonucleotide. The injected embryo developed normally through the embryonic and bipinnaria stages but was unable to form normal brachioraria. This result suggests that the unique protein is involved in the morphogenetic event at the latter period in the starfish ontogeny.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Petroacetylene, a new polyacetylene from the marine sponge Petrosia solida that inhibits blastulation of starfish embryos.2013
Author(s)
Ohta, S., Ogawa, T., Ohta, E., Ikeuchi, T., Kamemura, K., and Ikegami, S.
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Journal Title
Natural Product Research
Volume: 27
Issue: 20
Pages: 1842-1847
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of O-GlcNAcylation in Starfish (Asterina pectinifera) Development from Fertilization to Bipinnaria Larva2011
Author(s)
Ogawa, M., Adachi, T., Ikegami, S., Kato, K. H., Yamamoto, A., & Kamemura, K
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 2
Pages: 358-361
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Uneven distribution pattern and increasing numbers of mesenchyme cells during development in the starfish, Asterina pectinifera2011
Author(s)
Hamanaka, G., Hosaka, E., Kuraishi, R., Hosoya, N., Matsumoto, M., & Kaneko, H
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Journal Title
Dev. Growth Differ
Volume: Vol. 53
Issue: 3
Pages: 440-449
DOI
NAID
Related Report
Peer Reviewed
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