Project/Area Number |
23658155
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
General fisheries
|
Research Institution | Hokkaido University |
Principal Investigator |
HOSOKAWA Masashi 北海道大学, 大学院・水産科学研究院, 准教授 (10241374)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 微生物 / 光刺激 / カロテノイド / 化学合成細菌 / 青色光 / isopentenylsaproxanthin / Jejuia pallidilutea / 海洋細菌 / ゼアキサンチン / Flavobacteria / Algibacter lectus |
Research Abstract |
11ShimoA1, which was isolated from a marine alga, synthesized a novel carotenoid,2’-isopentenyl saporxanthin (2’-isopentenyl Spx). Blue LED irradiation increased2’-isopentenyl Spx content in the culture medium of strain A1. Furthermore, blue LED also enhanced mRNA expression level of carotenoid synthetic genes such as crtB、crtI、crtY、crtZ. In addition, strain A1 has unique genes coded prenyltransferase and phytoene desaturase, which are suggested to play an important role for 2’-isopentenyl Spx synthesis in strain A1. Both genes also tended to be up-regulated by blue LED. These results show that strain A1 is a unique bacteria with carotenoid synthetic pathways regulated by blue light.
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