Preservation of endangered and rare animalspecies using induced pluripotent stem cells.

• Project/Area Number: 23658224
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Kyoto University
• Principal Investigator: IMAI Hiroshi 京都大学, 大学院・農学研究科, 教授 (10303869)
• Co-Investigator(Kenkyū-buntansha): OKADA Kounosuke 日本獣医生命科学大学, 応用生命科学部, 講師 (60445830)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 野生動物保護・増殖 / 多能性幹細胞

Research Abstract

The establishment of induced pluripotent stem (iPS) cells in vole and the characterizations of the established cell lines hasbeen performed as a model experiment for the preservation of endangered and rare animal species. Pluripotent-related four transcription genes were introduced into somatic cells using retroviral vectors. Colonies like as mouse embryonic stem (ES) cells were obtained. These colonies have a part of stem cell potential, but have limited pluripotent characteristics. Further experiments are necessary to establish a system inducing nuclear reprogramming of somatic cells in vole.

Research Products

• [Journal Article] Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycyclin-inducible factors and an EOS reporter system (2011)
  • Author(s): Tsukiyama T, Asano R, Kawaguchi T, Kim N, Yamada M, Minami N, Ohinata H and Imai H
  • Journal Title: Genes Cells Volume: 16 Pages: 815-825
  • NAID: 120005593680
  • Peer Reviewed
• [Journal Article] 哺乳動物における iPS 細胞
  • Author(s): 浅野 良太、川口 高正、今井 裕
  • Journal Title: 日本胚移植学雑誌 Volume: 34巻、1号 Pages: 9-18
  • NAID: 10030763814
  • URL: http://jets.kenkyuukai.jp/about/index.asp?
  • Peer Reviewed
• [Presentation] ウシにおけるマウス ES 細胞様 iPS 細胞の樹立の試み (2013)
  • Organizer: 第1回 関西生殖医学集談会、ハービスプラザ
  • Place of Presentation: 大阪
  • Year and Date: 2013-03-02
• [Presentation] Generation of murine-ES-cell-like iPS cells in cattle using piggyBac transposition of doxycycline-inducible transcription factor (2013)
  • Organizer: CiRA International Symposium 2013 ~Rising the next generation of stem cell research~
  • Place of Presentation: Kyoto, Japan
• [Presentation] Generation of murine-ES-cell-like iPS cells in cattle using piggyBac transposition of doxycycline-inducible transcription factor (2013)
  • Author(s): Imai H
  • Organizer: CiRA International Symposium 2013 ~Raising the next generation of stem cell research~
  • Place of Presentation: Kyoto, Japan
• [Presentation] Derivation of Trophoblast-Like Cells from Bovine Amnion Cells by Piggybac Transposon Vectors Carring Doxycycline-Inducible Reprogramming Factors (2012)
  • Organizer: The 62^<nd> Annual Meeting of Korean Society of Reproductive Medicine
  • Place of Presentation: Seoul Women's University, Seoul, Korea
  • Year and Date: 2012-12-01
• [Presentation] Derivation of murine-ES-cell like iPS cells in canine under the EOS reporter system (2012)
  • Organizer: International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Yokohama, Japan
• [Presentation] Transdifferentiation of bovine aminion cells into trophoblast-like cells using piggyBac transposition of doxycycline-inducible reprogramming factors (2012)
  • Organizer: Satellite symposium to the International Congress on Animal Reproduction (ICAR)
  • Place of Presentation: Banff, Canada
• [Presentation] Derivation of trophoblast-like cells from bovine amnion cells by piggyBac transposon vectors Carring Doxycycline-Inducible Reprogramming Factors (2012)
  • Author(s): Imai H
  • Organizer: The 62nd Annual Meeting of Korean Society of Reproductive Medicine
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Establishment of induced pluripotent stem cells in cattle using piggyBac transposition of doxycycline-inducible transcription factors (2011)
  • Organizer: The World Congress on Reproductive Biology (WCRB)
  • Place of Presentation: Cairns, Australia
• [Remarks] ホームページ
  • URL: http://www.reprod.kais.kyoto-u.ac.jp/
• [Remarks] 京都大学大学院農学研究科応用生物科学専攻生殖生物学分野
  • URL: http://www.reprod.kais.kyoto-u.ac.jp/index.htm
• [Remarks]
  • URL: http://www.reprod.kais.kyoto-u.ac.jp/
• [Patent(Industrial Property Rights)] 多能性幹細胞を効率的に作製する方法 (2013)
  • Inventor(s): 今井 裕・金 ナレ
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 2013-014443
  • Filing Date: 2013-01-29
• [Patent(Industrial Property Rights)] 多能性幹細胞を効率的に作製する方法 (2013)
  • Inventor(s): 今井裕・金ナレ
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-014443
  • Filing Date: 2013-01-29