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Roles of novel genes encoding secreted proteins in morphogenesis

Research Project

Project/Area Number 23659035
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionKyoto University

Principal Investigator

ITOH Nobuyuki  京都大学, 大学院・薬学研究科, 教授 (10110610)

Co-Investigator(Renkei-kenkyūsha) MIYAKE Ayumi  京都大学, 大学院・薬学研究科, 講師 (40346044)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分子物学 / 分泌性因子 / 形態形成 / 遺伝子 / ゼブラフィッシュ / マウス
Research Abstract

We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish including Brorin, Brorin-like, Neucrin, and Fgf22. The inhibition of Brorin and Brorin-like functions in zebrafish resulted in the impairment of neural development. Brorin-like potentially plays roles in neural development and functions. The knockdown of neucrin also suppressed neuronal differentiation and caused increased cell proliferation and apoptosis in developing neural tissues. Neucrin plays roles in neural development in zebrafish. The Fgf22 morphants were defective in proper formation of the MHB constriction and the midbrain. The knockdown of Fgf22 caused decreased cell proliferation in the midbrain, expanded expression of roof plate and tegmental marker genes, and decreased expression of tectal marker genes. Furthermore, Fgf22 partially rescued the Fgf3/fgf8 double morphant phenotype. Fgf22 is involved in midbrain development downstream of Fgf3 and Fgf8 in the MHB but not of Hh in the floor plate.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (3 results)

All 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results)

  • [Journal Article] Fgf22 regulated by Fgf3/Fgf8 signaling is required for zebrafish midbrain development2013

    • Author(s)
      A. Miyake, N. Itoh
    • Journal Title

      Biology Open

      Volume: 印刷中 Issue: 5 Pages: 515-524

    • DOI

      10.1242/bio.20134226

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neucrin a novel secreted antagonist of canonical Wnt signaling2012

    • Author(s)
      A. Miyake, S. Nihno, Y. Murakoshi, A. Satsuka, Y. Nakayama, N. Itoh
    • Journal Title

      plays roles in developing neural tissues in zebra fish、Mechanisms of Development

      Volume: 128 Issue: 11-12 Pages: 577-590

    • DOI

      10.1016/j.mod.2012.01.001

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fgf20b is required for the ectomesenchymal fate establishment of cranial neural crest cells in zebra fish Biochem2011

    • Author(s)
      H. Yamauchi, M. Goto, M. Katayama, A. Miyake, N. Itoh
    • Journal Title

      Biophys. Res. Commun

      Volume: 409 Issue: 4 Pages: 705-710

    • DOI

      10.1016/j.bbrc.2011.05.069

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed

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Published: 2011-08-05   Modified: 2019-07-29  

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