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Development of a novel nucleic acid drug targeting sense/antisense transcript pairs

Research Project

Project/Area Number 23659080
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionOsaka University

Principal Investigator

SAKURAI Fuminori  大阪大学, 大学院・薬学研究科, 准教授 (70370939)

Co-Investigator(Renkei-kenkyūsha) OBIKA Satoshi   (80243252)
Research Collaborator MIZUGUCHI Hiroyuki  大阪大学, 大学院・薬学研究科, 教授
TACHIBANA Masashi  大阪大学, 大学院・薬学研究科, 助教
KATAYAMA Kazufumi  大阪大学, 大学院・薬学研究科, 助教
GOTO Hitoshi  大阪大学, 大学院・薬学研究科, 大学院生
NANJO Yuki  大阪大学, 大学院・薬学研究科, 大学院生
TANINO Fumihito  大阪大学, 大学院・薬学研究科, 大学院生
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords非コード RNA / 核酸医薬 / 遺伝子発現制御 / がん / 非翻訳領域 / RNA activation / 非コードRNA
Research Abstract

Recent studies have demonstrated that small duplex RNA complementary to the promoter region of target gene enhances expression of target genes. This phenomenon is designated as “RNA activation”. In this study, we examined whether expression of the matrix metalloproteinase (MMP) inhibitor RECK is activated in the cultured tumor cell lines by transfection with small duplex RNA (dsRNA) complementary to the promoter of RECK gene, leading to the suppression of expression of MMPs and invasion of the tumor cells. Transfection with the dsRNAs significantly elevated RECK expression levels by approximately 6-fold in human tumor cells. Furthermore, the dsRNA-induced elevation of RECK expression resulted in reduction in expression of MMPs and invasion of tumor cells. These results suggest that small duplex RNA complementary to the promoter region of tumor suppressor genes would have the potential as a novel antitumor agent.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Products Report
  • Research Products

    (9 results)

All 2014 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Upregulation of RECK gene expression by small double-stranded RNA targeting the promoter region2014

    • Author(s)
      Sakurai F, Nanjo Y, et al.
    • Journal Title

      Cancer Gene Therapy

      Volume: 21 Issue: 4 Pages: 164-170

    • DOI

      10.1038/cgt.2014.12

    • Related Report
      Products Report
    • Peer Reviewed
  • [Presentation] プロモーター領域を標的とした二本鎖RNA による RECK 遺伝子の発現活性化に関する検討2012

    • Author(s)
      櫻井文教、南條有紀、立花雅史、水口裕之
    • Organizer
      第28回日本 DDS 学会学術集会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] Activation of RECK gene expression by targeting promoter region with small duplex RNAs2012

    • Author(s)
      Fuminori Sakurai, Yuki Nanjo, Hitoshi Goto, Fumihito Tanino, Masashi Tachibana, Kazufumi Katayama, Hiroyuki Mizuguchi
    • Organizer
      Oligonucleotide Therapeutic Society 8thAnnual Meeting 2012
    • Place of Presentation
      USA, Boston
    • Related Report
      2012 Final Research Report
  • [Presentation] プロモーター領域を標的とした二本鎖RNAによるRECK遺伝子の発現活性化に関する検討2012

    • Author(s)
      櫻井文教
    • Organizer
      第28回日本DDS学会学術集会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] プロモーター領域を標的とした二本鎖RNAによるRECK遺伝子の発現活性化2011

    • Author(s)
      南條有紀、櫻井文教、谷野文仁、立花雅史、水口裕之
    • Organizer
      第61回日本薬学会近畿支部総会・大会
    • Place of Presentation
      神戸
    • Year and Date
      2011-10-22
    • Related Report
      2012 Final Research Report
  • [Presentation] プロモーター領域を標的とした二本鎖RNAによるRECK遺伝子の発現活性化2011

    • Author(s)
      南條有紀、櫻井文教、谷野文仁、立花雅史、水口裕之
    • Organizer
      アンチセンス・遺伝子・デリバリーシンポジウム2011
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] プロモーター領域を標的とした二本鎖RNAによるRECK遺伝子の発現活性化2011

    • Author(s)
      南條有紀、櫻井文教、谷野文仁、立花雅史、水口裕之
    • Organizer
      アンチセンス・遺伝子・デリバリーシンポジウム2011
    • Place of Presentation
      大阪大学コンベンションセンター
    • Related Report
      2011 Research-status Report
  • [Presentation] プロモーター領域を標的とした二本鎖RNAによるRECK遺伝子の発現活性化2011

    • Author(s)
      南條有紀、櫻井文教、谷野文仁、立花雅史、水口裕之
    • Organizer
      第61回日本薬学会近畿支部総会・大会
    • Place of Presentation
      神戸学院大学ポートアイランドキャンパス
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      https://sites.google.com/site/bunshiseibutugaku/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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