Experimental approach for direct transformation of GABAergic neurons

• Project/Area Number: 23659092
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Fukui
• Principal Investigator: SATO Makoto 福井大学, 医学部, 教授 (10222019)
• Co-Investigator(Kenkyū-buntansha): KURODA Kazuki 福井大学, 医学部, 助教 (60557966) ; KOMADA Munekazu 愛知学院大学, 歯学部, 助教 (90523994)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 解剖学 / 分化 / 細胞 / 組織 / 神経科学 / 細胞・組織 / 脳・神経

Research Abstract

（１） Cortical neurons that had been transfected with Ptf1a expressing vector migrated tangentially into the cortex, of which migratory behavior is typical for GABAergic neurons.
（２）Ptf1a expression did not result in transformation of glutamatergic neurons into GABAergic neurons; no specific GABAergic neuronal markers were newly expressed in glutamatergic neurons by Ptf1a expression.
（３） We collected RNAs from Ptf1a expressed neurons and searched for any Ptf1a-induced genes, then we identified several candidate genes.

Research Products

• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration (2013)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M
  • Journal Title: Cerebral Cortex Volume: 23(6) Pages: 1410-1423
  • Peer Reviewed
• [Journal Article] Post-natal treatment by a blood-brain-barrier permeable calpain inhibitor, SNJ1945 rescued defective neuronal migration and neuronal circuit formation in lissencephaly (2013)
  • Author(s): Toba, S., Tamura, Y., Kumamoto, K., Yamada, M., Takao, K., Hattori, S., Miyakawa, T., Kataoka, Y., Azuma, M.,Hayasaka, K., Amamoto, M., Tominaga, K., Wynshaw-Boris, A., Wanibuchi, H., Oka, Y., Sato, M., Kato, M. and Hirotsune, S.
  • Journal Title: Sci. Rep Volume: 3 Pages: 1224-1224
  • Peer Reviewed
• [Journal Article] Post-natal therapeutic intervention for lissencephaly using a blood-brain-barrier permeable calpain inhibitor (2013)
  • Author(s): Shiori Toba, Yasuhisa Tamura, Kanako Kumamoto, Masami Yamada, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa, Yosky Kataoka, Mitsuyoshi Azuma, Kiyoshi Hayasaka, Masano Amamoto, Keiko Tominaga, Anthony Wynshaw-Boris, Mitsuhiro Kato and Shinji Hirotsune
  • Journal Title: SNJ1945 Scientific Reports Volume: 3 Issue: 1 Pages: 1224-1224
  • DOI: 10.1038/srep01224
  • Peer Reviewed
• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration (2013)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
  • Journal Title: Cerebral Cortex Volume: (in press) Issue: 6 Pages: 1410-1423
  • DOI: 10.1093/cercor/bhs123
  • Peer Reviewed
• [Journal Article] Long-term oxytocin administration improves social behaviors in a girl with autistic disorder. (case report) (2012)
  • Author(s): Kosaka, H., Munesue, T., Ishitobi, M., Asano, M., Omori, M., Sato, M., Tomoda, A. and Wada, Y
  • Journal Title: BMC Psychiatry Volume: 12(1) Pages: 110-110
  • Peer Reviewed
• [Journal Article] Activation of Aurora-A is essential for neuronal migration via modulation of microtubule organization (2012)
  • Author(s): Takitoh, T., Kumamoto, K., Wang C.-C., Sato, M., Toba, S., Wynshaw-Boris, A. and Hirotsune, S
  • Journal Title: J. Neurosci Volume: 32(32) Pages: 11050-11066
  • Peer Reviewed
• [Journal Article] Effects of antidepressants and mood stabilizers on serum levels of adiponectin (2012)
  • Author(s): Omata, N., Murata, T., Narita, K., Maruoka, N., Mitsuya, H., Mita, K., Nishimoto, T., Sato, M. and Wada, Y
  • Journal Title: NeuroEndocrinol. Lett. Volume: 33(1) Pages: 1-2
  • Peer Reviewed
• [Journal Article] Maternal bisphenol A oral dosing relates to the acceleration of neurogenesis in the developing neocortex of mouse fetuses (2012)
  • Author(s): Komada, M., Asai, Y ., Morii, M., Matsuki, M., Sato, M. and Nagao, T
  • Journal Title: Toxicology. Volume: 16;295(1-3) Pages: 31-8
  • Peer Reviewed
• [Journal Article] A tightly controlled conditional knockdown system using the tol2 transposon-mediated technique (2012)
  • Author(s): Iguchi, T., Yagi, H., Wang, CC. and Sato, M
  • Journal Title: PLoS ONE Volume: 7(3)
  • Peer Reviewed
• [Journal Article] GPR98/Gpr98 Gene Is Involved in the Regulation of Human and Mouse Bone Mineral Density (2012)
  • Author(s): Urano, T., Shiraki, M,. Yagi, H., Ito, M., Sasaki, N., Sato, M., Ouchi, Y . and Inoue, S
  • Journal Title: J. Clin. Endocrinol. Metab Volume: 97(4)
  • Peer Reviewed
• [Journal Article] Effects of antidepressants and mood stabilizers on serum levels of adiponectin. (2012)
  • Author(s): Omata, N. et al.
  • Journal Title: NeuroEndocrinol. Lett. Volume: 33(1) Pages: 1-2
  • Peer Reviewed
• [Journal Article] Activation of Aurora-A is essential for neuronal migration via modulation of microtubule organization. (2012)
  • Author(s): Takitoh, T. et al.
  • Journal Title: J. Neurosci. Volume: 32(32) Issue: 32 Pages: 11050-11066
  • DOI: 10.1523/jneurosci.5664-11.2012
  • Peer Reviewed
• [Journal Article] Long-term oxytocin administration improves social behaviors in a girl with autistic disorder (2012)
  • Author(s): Kosaka H et al.,
  • Journal Title: BMC Psychiatry Volume: 12(1) Issue: 1 Pages: 110-110
  • DOI: 10.1186/1471-244x-12-110
  • Peer Reviewed
• [Journal Article] GPR98/Gpr98 Gene Is Involved in the Regulation of Human and Mouse Bone Mineral Density (2012)
  • Author(s): Urano, T., Shiraki, M, . Yagi, H., Ito, M., Sasaki, N., Sato, M., Ouchi, Y. and Inoue, S.
  • Journal Title: J. Clin. Endocrinol. Metab Volume: 97(4) Issue: 4 Pages: E565-E574
  • DOI: 10.1210/jc.2011-2393
  • Peer Reviewed
• [Journal Article] A tightly controlled conditional knockdown system using the tol2 transposon-mediated technique (2012)
  • Author(s): Iguchi, T., Yagi, H., Wang, CC. and Sato, M.
  • Journal Title: PLoS ONE Volume: 7(3) Issue: 3 Pages: e33380-e33380
  • DOI: 10.1371/journal.pone.0033380
  • Peer Reviewed
• [Journal Article] Maternal bisphenol A oral dosing relates to the acceleration of neurogenesis in the developing neocortex of mouse fetuses (2012)
  • Author(s): Komada, M., et al
  • Journal Title: Toxicology Volume: 295(1-3) Issue: 1-3 Pages: 31-8
  • DOI: 10.1016/j.tox.2012.02.013
  • Peer Reviewed
• [Journal Article] iPS 細胞研究とその周辺技術 (2011)
  • Author(s): 黒田一樹, 佐藤 真
  • Journal Title: 特集 1 神経系における iPS 細胞(iPS 細胞の活用も含めた神経機能修復の現状と将来):脳 21 Volume: Vol.14 No.3
• [Journal Article] iPS細胞研究とその周辺技術、「特集1神経系におけるiPS細胞（iPS細胞の活用も含めた神経機能修復の現状と将来）」 (2011)
  • Author(s): 黒田 一樹 他
  • Journal Title: 脳21 Volume: 14（3）
  • Peer Reviewed
• [Presentation] マウス大脳皮質長連合ニューロン軸索の可視化の試み (2013)
  • Author(s): 岡 雄一郎,猪口徳一,佐藤 真
  • Organizer: ポスター(3P-G011)第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-30
• [Presentation] 神経細胞の棘突起(スパイン)形成における FILIP 関連分子の機能解析 (2013)
  • Author(s): 黒田一樹,謝 敏カク,猪口徳一,岡 雄一郎,尾身 実,王 振吉,八木秀司,佐藤 真
  • Organizer: ポスター(3P-G020)第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-30
• [Presentation] 非筋肉型ミオシン分子の細胞内局在を調節する新たな機構.ポスター(1P-G054) (2013)
  • Author(s): 八木秀司,野口光一,佐藤 真
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-28
• [Presentation] Filip is involved in the morphological control of dendritic spine through actomyosin regulation (2012)
  • Author(s): Yagi, H., Kuroda, K., Xie, M.-J. Ikeda, H., Iguchi, T., Komada, M., Murase K., Okabe M., Noguchi, K. and Sato, M
  • Organizer: ポスター(636.11/B30) Society For Neuroscience 2012
  • Place of Presentation: ニューオリンズ(U.S.A)
  • Year and Date: 2012-10-16
• [Presentation] A2-pancortins especially Pancortin-4, are involved in the neuronal cell desth after the ischemic stroke during the growth period (2012)
  • Author(s): Wang, C.-C., Kuroda, K., Xie, M.-J., Oka, Y., Iguchi, T., Matsuyama, T., and Sato, M
  • Organizer: ポ ス タ ー (P02-1) The 11thBiennial Meeting of the Asian-Pacific Society for Neurochemistry / 55thAnnual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-30
• [Presentation] Analyses on the potential candidates of collateral branch inducing factor that are specifically expressed in the targets of axon collaterals from the corticospinal projection. (2012)
  • Author(s): Iguchi, T., Oka, Y., Kuroda, K., Wang, C.-C., Xie, M.-J., Yagi, H., and Sato, M
  • Organizer: ポスター(P01-14)The 11thBiennial Meeting of the Asian-Pacific Society for Neurochemistry / 55thAnnual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-29
• [Presentation] FILIP-related molecule controls spine maturation in the hippcampal neuron via non-muscle myosin llb (2012)
  • Author(s): Kuroda, K., Yagi, H., Xie, M.-J., Oka, Y., Iguchi, T., and Sato, M
  • Organizer: ポスター(P01-36)The 11thBiennial Meeting of the Asian-Pacific Society for Neurochemistry / 55thAnnual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-29
• [Presentation] WAVE-Abi2 複合体は radial migration 時の成長円錐の活性化およびmultipolar-bipolar transition と glia-guided migration の開始を制御する (2012)
  • Author(s): 謝 敏カク,八木秀司, 黒田一樹,王 振吉,駒田致和,岡 雄一郎,猪口徳一,佐藤 真
  • Organizer: ポスター(P4-d05)第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-21
• [Presentation] マウス大脳皮質長連合ニューロン特異的遺伝子の同定 (2012)
  • Author(s): 岡 雄一郎,猪口徳一,佐藤 真
  • Organizer: ポスター(P2-c13)第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] FILIP によるアクチンミオシン調節能の棘突起動態変化への関与 (2012)
  • Author(s): 八木秀司,黒田一樹,謝 敏カク,永野隆,池田 弘,猪口徳一,村瀬一之,岡部 勝,佐藤 真
  • Organizer: ポスター(P2-f22)第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] 大脳皮質形成とスパイン形成の新たな共通分子基盤とその展開 (2012)
  • Author(s): 佐藤 真
  • Organizer: シンポジウム(S19-3)「神経発生・形成における細胞骨格の意義」第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] 「こどものこころを考える（分子脳機能から発達障害まで）」 (2012)
  • Author(s): 佐藤 真
  • Organizer: 大阪科学研究会「最先端医療科学学術シンポジウム―基礎研究から臨床応用まで―」(招待講演)
  • Place of Presentation: 大阪
• [Presentation] 皮質脊髄路の発達における軸策側枝形成因子の探索 (2012)
  • Author(s): 猪口 徳一他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] マイクロアレイ解析による大脳皮質長連合線維特異的遺伝子の同定 (2012)
  • Author(s): 岡 雄一郎他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] 神経細胞の棘状突起（スパイン）形成におけるFILIP関連分子の機能解析 (2012)
  • Author(s): 黒田 一樹他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] 大脳皮質形成とスパイン形成の新たな共通分子基盤とその展開 (2012)
  • Author(s): 佐藤 真
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] 大脳皮質における層構造異常とドパミン作動性神経投射との関連の解明 (2012)
  • Author(s): 竹田 健彦他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling (2011)
  • Author(s): 佐藤 真
  • Organizer: AACL -2011 (Asian Aging Core for Longevity) 長崎シンポジウム Japan-Korea Joint Conference on Brain Aging and Neurodegeneration
  • Place of Presentation: 長崎
  • Year and Date: 2011-11-21
• [Presentation] Dendritic growth cone activities, regulated by Abl kinase and Cdk5 via WAVE2-Abi2, are essential for completing multipolar-bipolar transition and starting glia-guided locomotion (2011)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Iguchi, T. and Sato, M.
  • Organizer: ポスター(A4 540.04)Society For Neuroscience 201
  • Place of Presentation: ワシントン (U.S.A)
  • Year and Date: 2011-11-15
• [Presentation] dendritic growth cone activity regulated by Abl kinase and Cdk5 via WAVE2-Abi2 is essential for completing the multipolar-bipolar transition and initiating glia-guided locomotion (2011)
  • Author(s): 佐藤 真
  • Organizer: シンポジウム(S06-2)「神経形態制御から機能へ -スペーシャルセルバイオロジーの観点から」第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
  • Year and Date: 2011-09-27
• [Presentation] Intermediate progenitorの増殖・分化におけるHedgehogシグナルの役割 (2011)
  • Author(s): 駒田 致和他
  • Organizer: 第51回日本先天異常学会学術集会
  • Place of Presentation: 東京
• [Presentation] 福井大学での最近のデータ紹介 (2011)
  • Author(s): 佐藤 真
  • Organizer: ORIGIN神経科学研究会夏のワークショップ
  • Place of Presentation: 兵庫
• [Presentation] 大脳皮質でのglia-guided locomotionの開始には、樹状突起の成長円錐が重要である (2011)
  • Author(s): 謝 敏カク他
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 中間前駆細胞の増殖・分化におけるヘッジホッグシグナルの役割 (2011)
  • Author(s): 駒田 致和他
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 神経棘突起の形態調節におけるFILIPの役割 (2011)
  • Author(s): 八木 秀司他
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Filip play a role in cortical development and spine morphology (2011)
  • Author(s): 八木 秀司他
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
• [Presentation] Dendritic growth cone activity regulated by Abl kinase and Cdk5 via WAVE2-Abi2 is essential for completing the multipolar-bipolar transition and initiating glia-guided locomotion. (2011)
  • Author(s): 佐藤 真
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
• [Presentation] Dendritic growth cone activities, regulated by Abl kinase and Cdk5 via WAVE2-Abi2, are essential for completing multipolar-bipolar transition and starting glia-guided locomotion. (2011)
  • Author(s): Xie, M.-J. et al.
  • Organizer: Society For Neuroscience 2011
  • Place of Presentation: ワシントン
• [Presentation] Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling. (2011)
  • Author(s): 佐藤 真
  • Organizer: AACL -2011 (Asian Aging Core for Longevity）長崎シンポジウム(招待講演)
  • Place of Presentation: 長崎
• [Presentation] マウス大脳皮質長連合ニューロン特異的遺伝子の同定
  • Author(s): 岡 雄一郎他
  • Organizer: 嗅覚情報処理の神経基盤 －匂い分子から嗅覚神経回路、行動・情動まで－（包括型脳科学研究推進支援ネットワーク支援ワークショップ）
  • Place of Presentation: 東京
• [Presentation] マウス大脳皮質長連合ニューロン特異的遺伝子の同定
  • Author(s): 岡 雄一郎他
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] FILIPによるアクチンミオシン調節能の棘突起動態変化への関与
  • Author(s): 八木 秀司他
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] WAVE-Abi2複合体はradial migration時の成長円錐の活性化およびmultipolar-bipolar transitionとglia-guided migrationの開始を制御する
  • Author(s): 謝 敏カク他
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Analyses on the potential candidates of collateral branch inducing factor that are specifically expressed in the targets of axon collaterals from the corticospinal projection.
  • Author(s): Iguchi, T. et. al.
  • Organizer: The 11th Biennial Meeting of the Asian-Pacific Society for Neurochemistry / 55th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
• [Presentation] FILIP-related molecule controls spine maturation in the hippcampal neuron via non-muscle myosin llb.
  • Author(s): Kuroda, K. et. al.
  • Organizer: The 11th Biennial Meeting of the Asian-Pacific Society for Neurochemistry / 55th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
• [Presentation] A2-pancortins especially Pancortin-4, are involved in the neuronal cell desth after the ischemic stroke during the growth period.
  • Author(s): Wang, CC. et. al.
  • Organizer: The 11th Biennial Meeting of the Asian-Pacific Society for Neurochemistry / 55th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
• [Presentation] Filip is involved in the morphological control of dendritic spine through actomyosin regulation
  • Author(s): Yagi, H. et. al.
  • Organizer: Society For Neuroscience 2012
  • Place of Presentation: ニューオリンズ（U.S.A.)
• [Presentation] Identification of long association neuron-specific genes in the mouse cerebral cortex.
  • Author(s): Oka, Y. et. al.
  • Organizer: International symposium on "Sensory Systems & Neural Circuits"
  • Place of Presentation: 東京
• [Presentation] 非筋肉型ミオシン分子の細胞内局在を調節する新たな機構
  • Author(s): 八木 秀司他
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
• [Presentation] 神経細胞の棘突起（スパイン）形成におけるFILIP関連分子の機能解析
  • Author(s): 黒田 一樹他
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
• [Presentation] マウス大脳皮質長連合ニューロン軸索の可視化の試み
  • Author(s): 岡 雄一郎他
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
• [Remarks]
  • URL: http://www.med.u-fukui.ac.jp/KAIBOU2/